[논문]향상된 광열 효과를 갖는 카르복실화된 환원 그래핀옥사이드-골드나노막대 나노복합체의 제조 및 특성 분석

이승화; 김소연

doi:10.14478/ace.2021.1034

향상된 광열 효과를 갖는 카르복실화된 환원 그래핀옥사이드-골드나노막대 나노복합체의 제조 및 특성 분석
Preparation and Characterization of Reduced Graphene Oxide with Carboxyl Groups-Gold Nanorod Nanocomposite with Improved Photothermal Effect 원문보기

공업화학 = Applied chemistry for engineering, v.32 no.3, 2021년, pp.312 - 319

이승화 (충남대학교, 에너지과학기술대학원) , 김소연 (충남대학교, 에너지과학기술대학원)

초록
AI-Helper

광열 치료(photothermal therapy)란 빛을 조사하여 열을 발생시킴으로써 정상세포보다 열에 약한 비정상 세포, 특히 암세포를 선택적으로 괴사시키는 치료법이다. 본 연구에서는 광열 치료를 위한 카르복실화된 환원 그래핀옥사이드(reduced graphene oxide with carboxyl groups, CRGO)-골드나노막대(gold nanorod, AuNR) 나노복합체를 합성하고자 하였다. 이를 위해 그래핀옥사이드(graphene oxide, GO)를 고온에서 선택적으로 환원, 박리하여 CRGO를 합성하였고, AgNO₃의 양에 따라 AuNR의 길이를 조절하여 880 nm에서 강한 흡광 특성을 나타내는 AuNR를 합성하여 광열 인자로 사용하였다. 일반적인 방법으로 환원된 RGO에 비해 CRGO에 상대적으로 많은 카르복실기가 결합되어 있음을 FT-IR, 열 중량 분석 및 형광 분석을 통해 확인하였다. 또한, RGO에 비해 많은 carboxyl group이 결합된 CRGO는 수용액상에서 우수한 안정성을 나타내었다. 정전기적 상호작용을 통해 합성된 CRGO-AuNR 나노복합체는 약 317 nm의 균일한 크기와 좁은 크기 분포를 보였다. CRGO-AuNR 나노복합체는 두 가지 광열 인자인 CRGO와 AuNR의 synergistic effect로 인하여 조직 투과도가 우수한 근적외선 880 nm 레이저의 조사에 의한 광열 효과가 AuNR보다 2배 이상 향상 되는 것을 확인하였다. 또한, 광열 효과에 의한 암세포 독성 분석 결과, CRGO-AuNR 나노복합체가 가장 우수한 세포 독성 특성을 나타내었다. 따라서 CRGO-AuNR 나노복합체는 안정된 분산성과 향상된 광열 효과를 기반으로 항암 광열 요법 분야에 응용될 수 있을 것으로 기대된다.

Abstract ▼ AI-Helper

Photothermal therapy is a treatment that necrotizes selectively the abnormal cells, in particular cancer cells, which are more vulnerable to heat than normal cells, using the heat generated when irradiating light. In this study, we synthesized a reduced graphene oxide with carboxyl groups (CRGO)-gold nanorod (AuNR) nanocomposite for photothermal treatment. Graphene oxide (GO) was selectively reduced and exfoliated at high temperature to synthesize CRGO, and the length of AuNR was adjusted according to the amount of AgNO3, to synthesize AuNR with a strong absorption peak at 880 nm, as an ideal photothermal agent. It was determined through FT-IR, thermogravimetric and fluorescence analyses that more carboxyl groups were conjugated with CRGO over RGO. In addition, CRGO exhibited excellent stability in aqueous solutions compared to RGO due to the presence of carboxylic acid. The CRGO-AuNR nanocomposites fabricated by electrostatic interaction have an average size of ~317 nm with a narrow size distribution. It was confirmed that under radiation with a near-infrared 880 nm laser which has an excellent tissue transmittance, the photothermal effect of CRGO-AuNR nanocomposites was greater than that of AuNR due to the synergistic effect of the two photothermal agents, CRGO and AuNR. Furthermore, the results of cancer cell toxicity by photothermal effect revealed that CRGO-AuNR nanocomposites showed superb cytotoxic properties. Therefore, the CRGO-AuNR nanocomposites are expected to be applied to the field of anticancer photothermal therapy based on their stable dispersibility and improved photothermal effect.

주제어

표/그림 (9)

그림 Figure 1. Schematic illustration of the synthesis of the CRGO-AuNR nanocomposite.
그림 Figure 2. (A) UV absorption spectra of AuNRs according to the AgNO₃; (B) TEM image of (a) AuNR(670), (b) AuNR(780), and (c) AuNR(880).
그림 Figure 3. (A) FT-IR spectra of (a) GO, (b) RGO and CRGO; (B) TGA analysis curves of (a) graphite flake, (b) RGO, (c) CRGO, (d) GO; (C) photoluminescence of GO, RGO, and CRGO excited at 325 nm.
표 Table 1. The LSPR Peaks and Aspect Ratio of AuNRs according to the Amount of AgNO₃
그림 Figure 4. (A) Hydrodynamic size distribution of (a) CRGO before exfoliation, (b) CRGO after exfoliation, and (c) CRGO-AuNR nanocomposite; (B) dispersion stability of (a) AuNR, (b) GO, (c) RGO, (d) CRGO, (e) GO-AuNR composite, and (f) CRGO-AuNR nanocomposite.
그림 Figure 5. (A) UV absorption spectra of (a) AuNR, (b) GO, (c) CRGO and, (d) CRGO-AuNR nanocomposite; (B) TEM image of (a) GO, (b) CRGO, and (c) CRGO-AuNR nanocomposite.
그림 Figure 6. (A) Photothermal heating curve of (a) water, (b) GO, (c) CRGO, (d) AuNR, and (e) CRGO-AuNR nanocomposite with an 880 nm laser irradiation (1.0 W/cm²) for 10 min; (B) thermal image of (a) water, (b) GO, (c) CRGO, (d) AuNR, and (e) CRGO-AuNR nanocomposite with an 880 nm laser irradiation (1.0 W/cm²) for 10 min.
표 Table 2. Zeta Potential Measurement of Different Samples in Aqueous Solution
그림 Figure 7. (A) In vitro cytotoxicity of CRGO, AuNR, and CRGO-AuNR nanocomposite with various concentrations in A549 cell; (B) in vitro

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