[논문]Grapefruit seed extract와 7종의 Essential oil 및 혼합 Essential oil의 항균 활성

육영삼

doi:10.22156/cs4smb.2021.11.06.198

Grapefruit seed extract와 7종의 Essential oil 및 혼합 Essential oil의 항균 활성
Antibacterial activity of grapefruit seed extract and seven kinds of essential and blended essential oils 원문보기

융합정보논문지 = Journal of Convergence for Information Technology, v.11 no.6, 2021년, pp.198 - 205

육영삼 (단국대학교 보건복지대학원 임상의과학과)

초록
AI-Helper

본 배경 : 질염은 항생제를 복용하는 방식으로 치료를 하고 있으며, 이러한 항생제의 지속적인 치료는 내성을 유발할 수 있다. 연구 방법 : Lactic acid bacteria 2종에 에센셜 오일을 이용한 항균 활성을 보고자 한다. SE(Grapefruit Seed Extract), eucalyptus, tea tree, clove bud oil, cinnamon oil, lemongrass oil, thyme oil, ginger oil을 일정 비율로 넣어 배양 후, 병원성 미생물- E. coli, C. albicans와 Lactic acid bacteria은 균주에 맞는 배지를 사용하여 균 수를 측정하였다. 결과 : Essential oil 7종과 GSE가 병원성 미생물에 억제 효과가 있으며, 병원성균(E. coli, C. albicans)에 대한 Grapefruit seed extract(GSE)의 억제농도를 확인하였다, 병원성균은 억제하고 Lactic acid bacteria는 억제하지 않는 배합비도 확인하였다(GSE 농도가 200ppm에서 Eucalyptus globulus(EG) oil 50㎕와 Melaleuca alternifolia oil(Tea tree oil, TTO) 50㎕(pH 5.0, 5.5, 6.0)). 결론 : 본 실험에서 Essential oil은 다양한 항균 활성 가지고 있어 항생제 대안으로도 생각할 수 있으며, 장기 항생제 치료환자에 대한 보조 항균제로서도 유용할 것으로 생각된다.

Abstract ▼ AI-Helper

Objectives: Antibiotics help treat Vaginitis, and prolonged usage of antibiotics can lead to resistance. Methods: This study investigates the antimicrobial activity of two types of lactic acid bacteria using essential oils. After cultivation by adding grapefruit seed extract (GSE), eucalyptus, tea tree, clove bud, cinnamon, lemongrass, thyme, and ginger oils in a specific ratio, pathogenic microorganisms, namely E. coli, C. albicans, and lactic acid bacteria were released. The number of bacteria was measured using a medium suitable for the strains. Results: The essential oils and GSE inhibited pathogenic microorganisms, and the inhibitory concentration of GSE against pathogenic bacteria (E. coli, C. albicans) was confirmed. The non-inhibitory mixing ratio was also confirmed (50 μl of eucalyptus globulus (EG) oil and 50 μl of melaleuca alternifolia oil (tea tree oil, TTO) at 200 ppm GSE (pH 5.0, 5.5, 6.0)). Conclusion: Essential oils can be considered as an alternative to antibiotics because of their antibacterial properties. They are useful as auxiliary antibacterial agents for patients under long-term antibiotic treatment.

주제어

표/그림 (8)

표 Table 1. Strain and media used in this study
표 Table 2. Essential oils used in this study
표 Table 3. Antimicrobial activity of grapefruit seed extract by concentration according to pH _*4)
표 Table 4. Antibacterial activity of GSE and essential oils at pH 5.0
표 Table 5. Antimicrobial activity of GSE and two mixed essential oils at pH 5.0
표 Table 6. Antibacterial activity of GSE and 3 blended essential oils at pH 5.0
표 Table 7. Antibacterial activity of mixed eucalyptus oil and tea tree oil by pH _*1) when the concentration of GSE is 200ppm
표 Table 8. Antibacterial activity of GSE and 7 kinds of essential oils at pH 5.0

참고문헌 (29)

R. E Ley, D. A Peterson & J. I. Gordon. (2006). Ecological and evolutionary forces shaping microbial diversity in the human intestine. Cell, 124(4), 837-848. DOI : 10.1016/j.cell.2006.02.017

상세보기
A. Alok, I. D. Singh, S. Singh, M. Kishore, P. C. Jha, & A Iqubai. (2017). Probiotics: A new era of biotherapy. Advanced biomedical research, 6, 31. DOI : 10.4103/2277-9175.192625

상세보기
S Choi, SH Cho, & H Yi. (2016). Human microbiome studies in Korea. Allergy, Asthma & Respiratory Disease, 4 (5), 311-320. DOI : 10.4168/aard.2016.4.5.311

상세보기
Kimberly A. Workowski. (2015). Centers for disease control and prevention sexually transmitted diseases treatment guidelines. Clinical Infectious Diseases, 61 (suppl_8), S759-S762. DOI : 10.1093/cid/civ771

상세보기
D Nasioudis, IM Linhares, WJ Ledger, & Witkin. (2017). Bacterial vaginosis: a critical analysis of current knowledge. BJOG: An International Journal of Obstetrics & Gynaecology, 124 (1), 61-69. DOI : 10.1111/1471-0528.14209

상세보기
J Ravel, & RM Brotman. (2016). Translating the vaginal microbiome: gaps and challenges. Genome Medicine, 8 (1), 1-3. DOI : 10.1186/s13073-016-0291-2

상세보기
American College of Obstetricians and Gynecologists. (2006). ACOG practice bulletin: clinical management guidelines for obstetrician-gynecologists, Number 76, October 2006: postpartum hemorrhage, Obstetrics and Gynecology, 108(4), 1039-1047. DOI : 10.1097/00006250-200610000-00046

상세보기
H Moghim, S Taghipoor, N Shahinfard, S Kheiri, R Pana-hi. (2015). Antifungal effects of Zataria multiflora and Nigel-la sativa extracts against Candida albicans. Journal of HerbMed Pharmacology, 4, 138-141.
M Derda, & E Hadas. (2015). The use of phytotherapy in diseases caused by parasitic protozoa. Acta parasitological, 60(1), 1-8. DOI : 10.1515/ap-2015-0001

상세보기
P Hay. (2009). Recurrent bacterial vaginosis. Current Opinion in Infectious Diseases, 22, 82-86. DOI : 10.1097/QCO.0b013e32832180c6

상세보기
S. Sanchez, P. J. Garcia, K. K. Tomas, M. Catlin, & K. K. Holmes. (2004). Intravaginal metronidazole gel versus metronidazole plus nystatin ovules for bacterial vaginosis: a randomized controlled trial. American Journal of Obstetrics and Gynecology, 191 (6), 1898-1906. DOI : 10.1016/j.ajog.2004.06.089

상세보기
R. H. Beigi, M. N. Austin, L. A. Meyn, M. A. Krohn, & S. L. Hillier. (2004). Antimicrobial resistance associated with the treatment of bacterial vaginosis. American journal of obstetrics and gynecology, 191(4), 1124-1129. DOI : 10.1016/j.ajog.2004.05.033

상세보기
B. Foxman, R. Muraglia, J. P. Dietz, J. D. Sobel, & J. Wagner. (2013). Prevalence of recurrent vulvovaginal candidiasis in 5 European countries and the United States: results from an internet panel survey. Journal of lower genital tract disease, 17(3), 340-350. DOI : 10.1097/LGT.0b013e318273e8cf

상세보기
A. A. Oun & J. W. Rhim. (2020). Preparation of multifunctional carboxymethyl cellulose-based films incorporated with chitin nanocrystal and grapefruit seed extract. International Journal of Biological Macromolecules, 152, 1038-1046. DOI : 10.1016/j.ijbiomac.2019.10.191

상세보기
Y. J. Song, H. H. Yu, Y. J. Kim, N. K. Lee, & H. D. Paik. (2019). Anti-biofilm activity of grapefruit seed extract against Staphylococcus aureus and Escherichia coli. Journal of microbiology and biotechnology, 29(8), 1177-1183. DOI : 10.4014/jmb.1905.05022

원문보기 상세보기
M. Komura et al. (2019). Inhibitory effect of grapefruit seed extract (GSE) on avian pathogens. The Journal of veterinary medical science, 81(3), 466-472. DOI : 10.1292/jvms.18-0754

상세보기
M. N. Boukhatem, A. Boumaiza, H. G. Nada, M. Rajabi, & S. A. Mousa. (2020). Eucalyptus globulus essential oil as a natural food preservative: antioxidant, antibacterial and antifungal properties In Vitro and in a real food matrix (orangina fruit juice). Applied Sciences, 10(16), 5581. DOI : 10.3390/app10165581

상세보기
M. D. Neiva et al. (2020). Bark residues valorization potential regarding antioxidant and antimicrobial extracts. Wood Science and Technology, 54, 559-585. DOI: 10.1007/s00226-020-01168-3

상세보기
N Puvaca et al. (2020). Influence of different tetracycline antimicrobial therapy of mycoplasma (Mycoplasma synoviae) in laying hens compared to tea tree essential oil on table egg quality and antibiotic residues. Foods, 9(5), 612. DOI : 10.3390/foods9050612

상세보기
M. P. Silveira et al. (2020). Development of active cassava starch cellulose nanofiber-based films incorporated with natural antimicrobial tea tree essential oil. Journal of Applied Polymer Science, 137(21), 48726. DOI : 10.1002/app.48726

상세보기
P. Brun, G. Bernabe, R. Filippini & A. Piovan. (2019). In Vitro antimicrobial activities of commercially available tea tree (Melaleuca alternifolia) essential oils. Current Microbiology, 76, 108-116. DOI : 10.1007/s00284-018-1594-x

상세보기
M. Das, S. Roy, C. Guha, A. K. Saha, & M. Singh. (2020). In vitro evaluation of antioxidant and antibacterial properties of supercritical CO2 extracted essential oil from clove bud (Syzygium aromaticum). Journal of Plant Biochemistry and Biotechnology, 1-5. DOI : 10.1007/s13562-020-00566-9

상세보기
Y. Jung, H. Yang, I. Lee, T. S. Yong, S. Lee. (2020). Core/sheath-structured composite nanofibers containing cinnamon oil: their antibacterial and antifungal properties and acaricidal effect against house dust mites. Polymers, 12(1), 243. DOI : 10.3390/polym12010243

상세보기
J. Viktorova et al. (2020). Lemongrass essential oil does not modulate cancer cells multidrug resistance by Citral-its dominant and strongly antimicrobial compound. Foods. 9(5), 585. DOI : 10.3390/foods9050585

상세보기
S Lee, H Kim, LR Beuchat, Y Kim, JH Ryua. (2020). Synergistic antimicrobial activity of oregano and thyme thymol essential oils against Leuconostoc citreum in a laboratory medium and tomato juice. Food Microbiology, 90, 103489. DOI: 10.1016/j.fm.2020.103489

상세보기
A. Amalraj, J. T. Haponiuk, S. Thomas & S. Gopi. (2020). Preparation, characterization and antimicrobial activity of polyvinyl alcohol/gum arabic/chitosan composite films incorporated with black pepper essential oil and ginger essential oil. International Journal of Biological Macromolecules, 151, 366-375. DOI : 10.1016/j.ijbiomac.2020.02.176

상세보기
R. Denkova, V. Yanakieva, Z. Denkova, V. Nikolova & V. Radeva. (2013). In vitro inhibitory activity of bifidobacterium and lactobacillus strains against Candida albicans. Bulgarian Journal of Veterinary Medicine, 16(3), 186-197.
A. F. Cunha, L. B. Acurcio, B. S. Assis, D. L. Oliveira, M. O. Leite, M. M. Cerqueira & M. R. Souza. (2013). In vitro probiotic potential of Lactobacillus spp. isolated from fermented milks. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 65(6), 1876-1882. DOI : 10.1590/S0102-09352013000600040

상세보기
J. S. Kim, Y. S. Yuk & G. Y. Kim. (2019). Inhibition effect on pathogenic microbes and antimicrobial resistance of probiotics. Korean Journal of Clinical Laboratory Science, 51(3), 294-300. DOI : 10.15324/kjcls.2019.51.3.294

원문보기 상세보기

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증