[논문]Melanogenesis Inhibitory Activity of Epicatechin-3-O-Gallate Isolated from Polygonum amphibium L.

Lee, Young Kyung; Hwang, Buyng Su; Hwang, Yong; Lee, Seung Young; Oh, Young Taek; Kim, Chul Hwan; Nam, Hyeon Ju; Jeong, Yong Tae

doi:10.48022/mbl.2010.10012

Melanogenesis Inhibitory Activity of Epicatechin-3-O-Gallate Isolated from Polygonum amphibium L. 원문보기

Microbiology and biotechnology letters = 한국미생물·생명공학회지, v.49 no.1, 2021년, pp.24 - 31

Lee, Young Kyung (Nakdonggang National Institute of Biological Resources) , Hwang, Buyng Su (Nakdonggang National Institute of Biological Resources) , Hwang, Yong (Nakdonggang National Institute of Biological Resources) , Lee, Seung Young (Nakdonggang National Institute of Biological Resources) , Oh, Young Taek (Nakdonggang National Institute of Biological Resources) , Kim, Chul Hwan (Nakdonggang National Institute of Biological Resources) , Nam, Hyeon Ju (Nakdonggang National Institute of Biological Resources) , Jeong, Yong Tae (Nakdonggang National Institute of Biological Resources)

Abstract ▼ AI-Helper

This study aimed to investigate the melanogenesis inhibitory activity of epicatechin-3-O-gallate (ECG) isolated from Polygonum amphibium L. ECG was isolated from the ethanol extract of P. amphibium L, and its chemical structure was determined using spectroscopic methods such as LC-ESI-MS, 1D-NMR, and UV spectroscopy. ECG inhibited the melanogenesis of B16F10 cells in a dose-dependent manner. Particularly, it decreased the melanin content by 27.4% at 200 µM concentration, compared with the control, in B16F10 cells, without causing cytotoxicity. It is noteworthy that the expression of three key proteins, including tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor (MITF), involved in melanogenesis, is significantly inhibited by ECG. The ECG isolated in this study caused the inhibition of body pigmentation and tyrosinase activity in vivo in the zebrafish model. These results suggest that the ECG isolated from P. amphibium L. is an effective anti-melanogenesis agent.

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표/그림 (5)

그림 Fig. 1. ¹H (up) and ¹³C (down) NMR data and chemical structure of epicatechin-3-O-gallate of isolated extracts produced from the Polygonum. amphibium L..
그림 Fig. 2. Effect of compounds isolated from P. amphibium L. on melanogenesis in B16F10 cell. melanin contents was measured with triplicate experiment. The cells were cultured with 50 μM of compounds for 3 days. Each value is expressed a means ± SD of triplicate determinations. *p < 0.05, **p < 0.01 versus of control group.
그림 Fig. 3. Effects of ECG isolated from P. amphibium L. on melanogenesis in B16F10 cell. (A) melanin contents was measured with triplicate experiment. The cells were cultured with 25- 200 μM of ECG for 3 days. (B) Effect of cell cytotoxicity was measured with triplicate experiment. The cells were cultured with 0-400 μM of ECG for 3 days. Each value is expressed a means ± SD of triplicate determinations. *p < 0.05, **p < 0.01, ***p < 0.001 versus of control group.
그림 Fig. 4. Effects of ECG from P. amphibium L. on melanogenesis and expression of melanogenesis-related protein in B16F10 cells. The cells were cultured with 0-200 μM of ECG for 72 h. Whole-cell lysate were then subjected to western blot analysis using antibodies against tyrosinase, TRP-1, TRP-2, and MITF. Equal protein loading was confirmed using β-actin antibody.
그림 Fig. 5. Effects of ECG from P. amphibium L. on melanogenesis of zebrafish. Synchronized embryos were treated with melanogenic inhibitors at the indicated concentrations. ECG were dissolved in 0.1% DMSO then added to the embryo medium. The effects on the pigmentation of zebrafish were observed under the steromicroscope. (A) Pigmentation of zebrafish, (B) Total melanin content was quantified using spectrometer. (C) Tyrosinase activity was quantified using spectrometer. *p < 0.05, **p < 0.01, ***p < 0.001 versus of control group.

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Melanogenesis Inhibitory Activity of Epicatechin-3-O-Gallate Isolated from Polygonum amphibium L. 원문보기

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Melanogenesis Inhibitory Activity of Epicatechin-3-O-Gallate Isolated from Polygonum amphibium L. 원문보기

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