[논문]쌍발이모자반(Sargassum patens) 추출물의 항산화 및 항염효과

김숙희

doi:10.22156/cs4smb.2021.11.07.264

쌍발이모자반(Sargassum patens) 추출물의 항산화 및 항염효과
Antioxidant and Anti-inflammatory activity of Sargassum patens extract 원문보기

융합정보논문지 = Journal of Convergence for Information Technology, v.11 no.7, 2021년, pp.264 - 271

김숙희 (건국대학교 미래지식교육원 학점은행제 K뷰티산업융합학전공학과)

초록
AI-Helper

본 연구에서는 쌍발이모자반 추출물의 항산화능 및 항염능을 확인하였다. 항산화능 실험에는 폴리페놀 농도측정, 플라보노이드 농도 측정, DPPH 실험, ABTS 실험, NO 실험, FRAP 실험을 실시하였다. 폴리페놀의 경우 18.99±0.69 mg/g으로 나타났다. 플라보노이드의 경우 11.89±1.16 mg/g으로 나타났다. DPPH 실험에서는 19.78 mg ascorbic acid / g extract의 항산화능을 나타내었으며, ABTS 실험에서는 63.64 mg ascorbic acid / g extract의 항산화능을 나타내었으며, NO 실험에서는 7.966 mg ascorbic acid / g extract의 항산화능을 나타내었다. FRAP에서는 쌍발이모자반 추출물의 1 mg이 ascorbic acid 2.089 ㎍의 환원력을 보였다. 한편 세포실험에서는 세포 독성과 LPS로 유도된 염증에 대한 항염능을 알아보았다. 세포독성의 경우 모든 농도에서 80%이상의 세포 생존률을 보였으며, NO 생성 억제능의 경우 100 ㎍/mL 농도에서 30.64±0.23%의 염증 억제능을 보여 쌍발이모자반 추출물이 항염능을 가진 화장품 원료로서 사용가능함을 보였다.

Abstract ▼ AI-Helper

In this study, the antioxidant and anti-inflammatory properties of Sargassum patens extracts were identified. Antioxidant experiments included polyphenol concentration measurements, flavonoid concentration measurements, DPPH experiments, ABTS experiment NO experiments, and FRAP experiments. For polyphenols, 18.99±0.69 mg/g was shown. Flavonoids showed 11.89±1.16 mg/g. The DPPH experiment showed an antioxidant function of 19.78 mg ascorbic acid/g extract, the ABTS experiment showed an antioxidant function of 63.64 mg ascorbic acid/g extract, and the NO experiment showed an antioxidant function of 7.966 mg ascorbic acid/g extract. In FRAP, 1 mg of the moxibustion extract showed a reduction of 2.089 ㎍ of ascorbic acid. In the meantime, cell experiments showed cytotoxicity and anti-inflammatory functions against inflammation induced by LPS. In cytotoxicity experiments, Sargassum patens extracts showed a cell survival rate of more than 80% at all concentrations, and an inflammatory inhibition of 30.64±0.23% at a concentration of 100 ㎍/mL. These results indicate that Sargassum patens extract is available as an anti-inflammatory cosmetic material.

주제어

표/그림 (6)

표 Table 1. Total polyphenol and flavonoid concentration of Sargassum patens extract
그림 Fig. 1. DPPH radical scavenging activity of Sargassum patens extract
그림 Fig. 2. ABTS radical scavenging activity of Sargassum patens extract
표 Table 2. NO radical scavenging activity of Sargassum patens extract
표 Table 3. Cell survival rate of RAW 264.7 with Sargassum patens extract
표 Table 4. NO production rate of RAW 264.7 with Sargassum patens extract

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