[논문]사향의 크로마토그램 패턴 분석을 통한 품질비교 연구

조종운; 가오단; 강종성; 정효원; 박용기

doi:10.6116/kjh.2021.36.4.41.

사향의 크로마토그램 패턴 분석을 통한 품질비교 연구
Quality comparison of musk products using a chromatographic pattern analysis 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.36 no.4, 2021년, pp.41 - 50

조종운 (충남대학교 약학대학) , 가오단 (충남대학교 약학대학) , 강종성 (충남대학교 약학대학) , 정효원 (동국대학교 한의과대학) , 박용기 (동국대학교 한의과대학)

Abstract ▼ AI-Helper

Objectives : This study is to check the quality of Moschus products in Korean markets using a chromatographic analysis. Methods : We collected musk products, two genuine products from Russia (A, B), two authentic products from Hong Kong (C) and Korea (D), the Ministry of Food and Drug Safety (MFDS) standard (E), and two fire ants products (E, F). Results : For identification, TLC analysis of 60% ethanol extracts of each product showed that A, B, C, and E have the suitable patterns before color development at 365 nm UV light for the MFDS regulation in Korea. A clear red spot was observed from the E at Rf 0.6 with 365 nm UV light after color development, but this spot was not found in A and B. For the purity test, a distinct violet spot with Rf 0.87 was observed from the A and B in TLC analysis of methanol extracts of each product at white light, however, this spot was not matched with the impurities of E and F. In HPLC-UV pattern analysis, a similar peak pattern was shown in A, B and E, and similar peaks were observed C, although the similarity was weaker than that of A, B, and E. The F and G showed different peak patterns compared with the peak patterns of other samples. Conclusions : Hence, it is considered that the test methods need flexibility in application for identification and purity test depending on the type of sample.

주제어

표/그림 (9)

표 Table 1. Types and characteristics of domestic distribution musks used in this study
그림 Fig. 1. Separation of components in musk samples extracted with 60% ethanol on a TLC plate developed with touene·ethyl ether mixture (1 : 1, v/v) and colored by heating for 5 min at 105℃ after spay of 10 % sulfuric acid·ethanol solution. Samples: see text.
그림 Fig. 2. Photographs of a TLC plate with musk samples extracted with 60% ethanol before and after coloration with irradiation of UV 254 and 365 nm. Samples: see text.
그림 Fig. 3. Separation of components in musk samples extracted with methanol on a TLC plate developed with touene·ethyl ether mixture (1 : 1, v/v) and colored by heating for 5 min at 105℃ after spay of 10 % sulfuric acid·ethanol solution. Samples: see text.
그림 Fig. 4. Photographs of a TLC plate with musk samples extracted with methanol before and after coloration with irradiation of UV 254 and 365 nm. Samples: see text.
그림 Fig. 5. HPLC-UV chromatogram of musk samples extracted with 60% ethanol. Column; Optimapak C18, 5 ㎛ (4.6 ㎜ × 250 ㎜), Mobile Phase; (A) 0.1% formic acid in water, (B) 0.1% formic acid in methanol, Gradient; 0 min, 25 B%, 120 min, 100 B%, Detection; PDAD (190 ~ 800 nm, split 1.2 nm), Flow rate; 1 mL/min, Column temperature; 30 ℃, Injection volum; 50 ㎕, Samples: see text.
그림 Fig. 6. UV spectra of peaks 1 ~ 5 separated from musk samples
그림 Fig. 7. HPLC-Fluorescence chromatogram of musk samples extracted with 60% ethanol. HPLC conditions: Column; Optimapak C18, 5 ㎛ (4.6 ㎜ × 250 ㎜), Mobile Phase; (A) 0.1% formic acid in water (B) 0.1% formic acid in methanol, Gradient: 0 min, 25 B%, 120 min, 100 B%, Detection; FLD (excitation: 254 nm, emission: 365 nm), Flow rate; 1 mL/min, Column temperature; 30 ℃, Injection volume; 50 ㎕ Samples: see text.
표 Table 2. Domestic import status of musk products

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