[논문]풀무치(Locusta migratoria)의 난소 및 알 발육 특성

곽규원; 고현진; 김선영; 이경용; 윤형주

doi:10.5656/ksae.2021.01.1.088

풀무치(Locusta migratoria)의 난소 및 알 발육 특성
Developmental Characteristics of Ovary and Egg of Migratory Locust, Locusta migratoria (Orthoptera: Acrididae) 원문보기

한국응용곤충학회지 = Korean journal of applied entomology, v.60 no.2, 2021년, pp.175 - 183

곽규원 (농촌진흥청 국립농업과학원 농업생물부 곤충산업과) , 고현진 (농촌진흥청 국립농업과학원 농업생물부 곤충산업과) , 김선영 (농촌진흥청 국립농업과학원 농업생물부 곤충산업과) , 이경용 (농촌진흥청 국립농업과학원 농업생물부 곤충산업과) , 윤형주 (농촌진흥청 국립농업과학원 농업생물부 곤충산업과)

초록
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식용 및 사료용 곤충으로 활용되는 풀무치에 대한 기초 연구로서 발육 단계별 난소 및 알 발육 특성을 조사하였다. 난소발육을 조사한 결과, 풀무치는 1쌍의 난소와 90개 이상의 무영양실형 난소소관을 가지고 있었다. 난소 길이는 5령 약충보다 성충시기가 더 길었다. 성충 1일째 난소길이는 5령 1일보다 약 2.5배 길었다. 난소 길이는 성충 30일까지 증가한 후, 성충 35일부터 감소되었다. 난소소관 길이는 난소 길이의 1/2 정도로, 역시 난소 길이와 같은 경향을 보였다. 난소 및 난소소관 길이는 체중과 비례적인 상관관계가 있었다. 난소소관의 수는 94-104개로 성충 30일이 총 104개로 가장 많았고 35일부터 감소하였다. 성숙란 수 역시 성충 15일부터 확인할 수 있었고, 성충 30일이 50.6개로 가장 많았다. 수정낭의 크기는 또한 발육 단계에 따라 영향을 받았다. 난괴에서 알을 분리하여 산란 후 일별로 발육 특성을 조사한 결과, 산란 4일부터 무게 및 알의 크기가 급격하게 증가하였고, 산란 후 7일부터는 변화의 폭이 거의 없었다. 산란 7일부터 몸체의 구분이 가능했으며, 산란 10일에 부화되었다. 이상의 결과로 볼 때, 풀무치의 난소발육은 성충 30일이 가장 좋았고, 산란 10일경에 풀무치 1령 약충이 우화하는 것으로 판단된다.

Abstract ▼ AI-Helper

As a baseline study of (common name) Locusta migratoria (Orthoptera: Acrididae) to utilize as a food source, we investigated the developmental characteristics of ovaries and eggs. Locusta migratoria had a pair of ovaries and more than 90-104 panoistic ovarioles. The ovary length in the adult stage was longer than it is in the 5th nymph stage. The length on the first day of the adult stage was 2.5-fold longer than the first day of 5th instar. The ovary length showed a tendency to increase until the 30th day of the adult phase, but decreased from the 35th day. Ovarioles length was about half of the ovary length, showing a similar tendency to the variation of ovary length. The lengths of the ovaries and the ovarioles increased proportionally to body weight. The matured eggs were identifiable from the 15th day of the adult stage, and the number was the highest on the 30th day at 50.6 individuals. Spermatheca size was also affected by the ovary development. The ovaries of L. migratoria were highly matured until the 30th day of adult stage. Weight and size of eggs increased sharply from the 4th day after egg oviposition and remained from the 7th day after spawning. Based on the above results, we found that the ovary of L. migratoria was most developed on the 30th day of adult phase, and 1st nymph is being hatched on the 10th day of the oviposition.

주제어

표/그림 (9)

그림 Fig. 1. Weight of Locusta migratoria at developmental stage. Different letters indicate significant difference in weight at developmental stage based on the results of one-way ANOVA test (p < 0.0001).
그림 Fig. 2. Pictures showing the female reproductive organ of Locusta migratoria. L. migratoria have two ovaries (A), each consisting of over 90 ovarioles (B) and (C) show seperated ovarioles from ovary.
그림 Fig. 3. Number of ovarioles in left and right ovary of L. migratoria at developmental stage. There were not significant differences in number of ovarioles and matured egg at developmental stage based on the results of one-way ANOVA test (p < 0.05).
그림 Fig. 4. Length of ovary and ovariole of L. migratoria at developmental stage. Different letters indicate significant differences in length of ovary and ovariole at developmental stage based on the results of one-way ANOVA test (p < 0.0001).
그림 Fig. 5. Number of matured egg in left and right ovariole of L. migratoria at developmental stage. There were not significant differences in number of ovarioles and matured egg at developmental stage based on the results of one-way ANOVA test (p < 0.05).
그림 Fig. 6. Length and width of spermathaeca of L. migratoria at developmental stage. Different letters indicate significant differences in length and width of spermathaeca at developmental stage based on the results of one-way ANOVA test (p < 0.0001).
그림 Fig. 7. Egg mass of Locusta migratoria.
그림 Fig. 8. Weight (A), width (B) and length (C) of egg of L. migratoria at day after oviposition. Different letters indicate significant differences in width and length of egg at day after oviposition based on the results of one-way ANOVA test (p < 0.0001).
표 Table 1. Morphological change of egg at days after oviposition

참고문헌 (38)

Arai, T., 1977. Effects of the daily cycle of light and temperature on hatchability and hatching time in Metrioptera hime Furukawa (Orthoptera, Tettigonidae). Kontyu 45, 107-120.
Azizi, N., Ghadraoui, L.E., Petit, D., Fadil, F., Mohim, A., 2010. A simple diet for the rearing success of the desert locust, Schistocerca gregaria (Forskal, 1775) (Orthoptera, Acrididae). Bull. Soc. entomol. Fr. 115, 445-450.
Bar-Zev, A., Wajc, E., Cohen, E., Sapir, L., Applebaum, S.W., Emmerich, H., 1975. Vitellogenin accumulation in the fat body and haemolymph of Locusta migratoria in relation to egg maturation. J. Insect Physiol. 21, 1257-1263.

상세보기
Chen, Y., 1999. The locust and grasshopper pests of China, China Forestry Publishing House, Beijing, pp. 1-72.
Clark, J., Lange, A.B., 2001. Evidence of a neural loop involved in controlling spermathecal contractions in Locusta migratoria. J. Insect Physiol. 47, 607-616.

상세보기
Colbert, P.L., Spencer, R.J., Janzen, F.J., 2010. Mechanism and cost of synchronous hatching. Functional Ecol. 24, 112-121.
Davey, K.G., 1985. The female reproductive system. in: Comprehensive insect physiology, biochemistry and pharmacology. Kerkut G.A., Gilbert L.I. (Eds.) Pergamon Press, Oxford, pp. 15-36.
Davey, K.G., Sevala, V.L., Gordon, D.R.B., 1993. The action of juvenile hormone and antigonadotropin on the follicle cells of Locusta migratoria. Invertebr. Reprod. Dev. 24, 39-45.

상세보기
Durst, P., Johnson, D.V., Leslie, R.N., Shono, K., 2010. Forest insects as food: Humans bite back. RAP publication, Bangkok, pp. 1-4.
Farrow, R.A., Colless, D.H., 1980. Analysis of the interrelationships of geographical races of Locusta migratoria (Linnaeus) (Orthoptera: Acrididae), by numerical taxonomy, with special reference to sub-speciation, in the tropics and affinities of the Australian race. Acrida 9, 77-99.
Gijbels, M., Lenaerts, Cynthia, L., Broeck, J.V., Marchal, E., 2019. Juvenile hormone receptor Met is essential for ovarian maturation in the desert locust, Schistocerca gregaria. Sci. Rep. 9, 10797.

상세보기
Gullan, P.J., Cranston, P.S., 2017. The insects: An outline of entomology. Wiely-Blackwell, 3, New Jersey, p. 78.
Kennedy, J.S., 1949. A preliminary analysis of oviposition behavior by Locusta (Orthoptera, Acrididae) in relation to moisture. Proc. Roy. Soc. Lona. 89, 83-89.
Lange, A.B., 1992. The neural and hormonal control of locust oviducts and accessory structure. Adv. Comp. Endocrinol. 1, 109-115.
Lange, A.B., da Silva, R., 2007. Neural and hormonal control of muscular activity of the spermathecal in the locust, Locusta migratoria. Peptides 28, 174-184.
Lange, A.B., 2009. Neural mechanisms coordinating the female reproductive system in the locust. Front. Biosci. 14, 4401-4415.
Mekonnen, M.M., Hoekstra, A.Y., 2011. The green, blue and grey water footprint of crops and derived crop products. Hydrol. Earth Syst. Sci. 15, 1577-1600.
Minks, G., Hoffman, J.A., 1975. Biological aspects of juvenile hormone action in the adult Locusta migratoria. Arch. Neerl. Zool. 17, 175-258.
Nishide, Y., Suzuki, T., Tanaka, S., 2017a. Synchrony in the hatching of eggs in the desert locust Schistocerca gregaria (Orhoptera: Acrididae): Egg condition influences hatching time in the laboratory and under simulated field temperatures. Appl. Entomol. Zool. 52, 599-604.
Nishide, Y., Suzuki, T., Tanaka, S., 2017b. The hatching time of Locusta migratoria under outdoor conditions: role of temperature and adaptive significance. Physiol. Entomol. 42, 146-155.

상세보기
Oonincx, D.G., Van Itterbeeck, J., Heetkamp, M.J., Van den Brand, H., Van Loon, J.J., Van Huis, A., 2010. An exploration on greenhouse gas and ammonia production by insect species suitable for animal or human consumption. PLos One 5, e14445.

상세보기
Pener, M.P., 1991. Locust phase polyphenism and its endocrine relations. Adv. Insect Physiol. 23, 1-79.
Phipps, J., 1950. The maturation of the ovaries and the relation between weight and maturity in Locusta migratoria migratorioides (R. & F.). Bull. Ent. Res. 40, 539-557.
Ramos-Elorduy, J., Moreno, J.M., Vazquez, A.I., Landero, I., OlivaRivera, H., Camacho, V.H., 2011. Edible lepidoptera in Mexico: Geographic distribution, ethnicity, economic and nutritional importance for rural people. J. Ethnobiol. Ethnomed. 7, 3-22.
Riddiford, L.M., 2012. How does juvenile hormone control insect metamorphosis and reproduction? Gen. Comp. Endocrinol. 179, 477-484.

상세보기
Sakamoto, H., Tanaka, S., Hata, T., 2019. Identification of vibrational signals emitted by embryos of the migratory locust Locusta migratoria (Orthoptera: Acrididae) that induce synchronous hatching. Eur. J. Entomol. 116, 258-268.

상세보기
Sevala, V.L., Davey, K.G., Prestwich, G.D., 1995. Photoaffinity labeling and characterization of a juvenile hormone binding protein in the membranes of follicle cells of Locusta migratoria. Insect Biochem. Mol. Biol. 25, 267-273.
Shimizu, T., Masaki, S., 1997. Daily time of hatching in Nembiine crickets. Jap. J. Entomol. 65, 335-342.
Tanaka, S., 1992. The significance of embryonic diapause in a Japanese strain of the migratory Locust, Locusta migratoria (Orthoptera, Acrididae). Jpn. J. Ent. 60, 503-520.
Tanaka, S., 2017. Locusta migratoria (Orhoptera: Acrididae) embryos monitor neighboring eggs for hatching synchrony. J. Orthopt. Res. 26, 103-115.
Tauber, M.J., Tauber, C.A., Masaki, S., 1986. Seasonal adaptation of insects. Oxford University Press, London, pp. 1-411.
Uvarov, B.P., 1966. Grasshoppers and Locusts, Vol. 1. Cambridge University Press, Cambridge, p. 481.
Uvarov, B.P., 1977. Grasshoppers and Locusts, Vol. 2. Centre for Overseas Pest Research, London, p. 613.
Van Huis, A., 2010. An exploration on greenhouse gas and ammonia production by insect species suitable for animal or human consumption. PLos One 5, e14445.

상세보기
Van Huis, A., van Itterbeeck, J., Klunder, H., Mertens, E., Halloran, A., Muir, G., Vantomme, P., 2013 Edible insects: Future prospects for food and feed security. Food and Agriculture Organization of the United Nations. Rome, pp. 1-187.
Vincent, J.F.V., 1975. How dose the female locust dig her oviposition hole? J. Ent. 50, 175-181.
Wyatt, G.R., Braun, R.P., Zhang, J., 1996. Priming effect in gene activation by juvenile hormone binding protein in the membrane of follicle cells of Locusta migratoria. Insect Biochem. Mol. Biol. 25, 267-273.
Yamagishi, M., Tanaka, S., 2009. Overwintering biology and morphological characteristics of the migratory locust, Locusta migratoria after outbreaks on Iheya Island, Japan. Appl. Entomol. Zool. 44, 165-174.

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