[논문]해동피를 포함한 한약제제 HPL-01의 Dextran sodium sulfate 유도 궤양성 대장염 완화 효과

조형권; 김대성; 조성완; 신나래; 박영미; 이학용; 신동엽

doi:10.15188/kjopp.2021.02.35.2.64

해동피를 포함한 한약제제 HPL-01의 Dextran sodium sulfate 유도 궤양성 대장염 완화 효과
Anti-Colitic Effects of Herbal Medicine HPL-01 on Dextran Sulfate Sodium Induced Ulcerative Colitis in Rat 원문보기

동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, v.35 no.2, 2021년, pp.64 - 70

조형권 ((유)한풍제약) , 김대성 ((유)한풍제약) , 조성완 (건양대학교 제약생명공학과) , 신나래 (주식회사 인비보) , 박영미 (주식회사 인비보) , 이학용 (주식회사 인비보) , 신동엽 (주식회사 인비보)

Abstract ▼ AI-Helper

This study aimed to investigate the anti-inflammatory effects of herbal complex HPL-01 on dextran sodium sulfate(DSS) induced ulcerative colitis in rat. Adult male Sprague-Dawley rats were randomized and divided into six groups. Five groups, excluding the normal group, were orally administered orally HPL-01(50, 100, or 200 mg/kg) for 21 days, and acute colitis was induced during the last 7 days by 4% DSS in the drinking water. The HPL-01 administered DSS-treated rats exhibited significantly reduced colon macroscopic damage index and increased body weight and colonic length 7 days after DSS treatment. Additionally, these rats showed lower serum levels of the pro-inflammatory cytokines TNF-α and IL-6 than those treated only with DSS. HPL-01(100 or 200 mg/kg) also attenuated the DSS-induced increase in the number of white blood cells, granulocytes, and mid cells and improved intestinal damage. Taken together, these results suggest that HPL-01 is a promising anti-inflammatory agent that may be in the treatment of colitis.

주제어

표/그림 (8)

그림 Fig. 1. Animal model experimental procedure. HPL-01 (50, 100, 200 mg/kg), Sulfasalazine (100 mg/kg) were administered for whole period. From 14 to day 19, 4% DSS was added to drinking water to induce Ulcerative colitis.
그림 Fig. 2. Effect of HPL-01 on the changes of body weight in DSS-treated in Rats. The weekly body weight was checked weights. Ulcerative colitis in rats were induced by 5% DSS dissolved in the drinking water for 7 days. Rats were administered 4% DSS in drinking water for 7 days with or without HPL-01 (50, 100, 200 mg/kg/day up to 21 days). Sulfasalazine (100 mg/kg/day up to 21 days) was used as a positive control. The values in the same row with different superscript letters are significantly different, P<0.05. Values are represented mean±SEM (n=7).
표 Table 1. Criteria for macroscopic scoring of colonic damage
그림 Fig. 3. Effect of HPL-01 on colon length in DSS-treated in Rats. Colons were obtained 7 days after commencement of DSS administration. (A) Macroscopic features of the colons. (B) Colon lengths were measured. Normal group (without DSS), 4% DSS administration group (Control), and 4% DSS with HPL-01 at 50, 100, 200 mg/kg up to 21 days. 4% DSS with Sulfasalazine (Positive control) at 100 mg/kg up to 21 days. a-c Means with the different letters on the bars are significantly different, P<0.05. Data shown are represented mean±SEM (n=7).
그림 Fig. 4. Effects of HPL-01 on macroscopic score in DSS-induced colitis at day 7 after induction. Colons were obtained 7 days after commencement of DSS administration, and macroscopic score of mice colons were measured. ^a-d Means with the different letters on the bars are significantly different, P<0.05. Data shown are represented mean±SEM (n=7).
그림 Fig. 5. Effect of HPL-01 on DSS-induced TNF-α and IL-6 production. Level of (A) TNF-α, (B) IL-6 were significantly decreased to compare with the control group. ^a-d Means with the different letters on the bars are significantly different, P<0.05. Data shown are represented mean±SEM (n=7).
그림 Fig. 6. Changes on the complete blood count after HPL-01 treatments in DSS induced colitis in rats. (A) WBC, (B) Granulocytes, (C) Lymphocyte and (D) Mid cells. Compared with the control group. a-c Means with the different letters on the bars are significantly different, P<0.05. Data shown are represented mean±SEM (n=7).
그림 Fig. 7. Effect of HPL-01 on colorectal histology in rat with DSS-induced colitis (×40). (A) Normal, (B) Control, (C) HPL-01 50 mg/kg, (D) HPL-01 100 mg/kg, (E) HPL-01 200 mg/kg and (F) Positive control (Sulfasalazine 100 mg/kg). Arrow, area of goblet cells depletion and loss of crypt structure; sterisk(*), edema in loose connective tissue. Scale bar=100 μm.

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