[논문]주정 농도별 감국 추출물의 항산화 및 항염증 활성

강현; 박찬휘; 권상오; 이성규

doi:10.9721/kjfst.2021.53.4.416

주정 농도별 감국 추출물의 항산화 및 항염증 활성
Antioxidant and anti-inflammatory activities of Chrysanthemum indicum Linne extracts at different ethanol ratios 원문보기

한국식품과학회지 = Korean journal of food science and technology, v.53 no.4, 2021년, pp.416 - 422

강현 (단국대학교 임상병리학과) , 박찬휘 (단국대학교 임상병리학과) , 권상오 ((주)에스앤디 기업부설연구소) , 이성규 (단국대학교 임상병리학과)

초록
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본 연구에서는 주정 농도별로 추출된 감국에 대한 항산화 및 항염증 활성을 측정하였다. 추출 수율의 경우 50% 주정 추출물에서 33.6%로 가장 높은 수율을 보였다. 총 폴리페놀 함량의 경우 30%와 70% 주정 추출물이 각각 59.70, 61.35 mg GAE/g으로 다른 추출물에 비해 유의적으로 높은 함량을 보였고, 플라보노이드 함량은 30% 주정 추출물에서 57.94 mg QE/g으로 가장 높은 함량을 보였다. 항산화 활성은 70% 주정 추출물이 DPPH 라디칼 소거능(RC₅₀ 14.95 ㎍/mL), ABTS⁺ 라디칼 소거능(RC₅₀ 42.28 ㎍/mL)에서 가장 활성이 높았다. FRAP 활성은 30% 주정 추출물에서 다른 추출물과 비교해 유의적으로 높은 활성을 보였다. 주정 농도별 추출물의 항염 효과는 LPS로 유도된 RAW 264.7 세포에서 염증 매개인자인 NO 생성 억제를 측정한 결과, 세포독성 없이 30% 주정 추출물 200 ㎍/mL 농도에서 11.16 µM로 가장 우수한 NO 생성 억제를 보였다. 또한, iNOS 발현을 측정한 결과 30-70% 주정 추출물이 높은 iNOS 발현 저해 효과를 보였다. 따라서 감국 추출물 제조 시 항산화 효과를 위해서는 30% 주정 및 70% 주정 으로 추출하는 것이 효과적이며 항염 효과를 위해서는 30-70% 주정으로 추출하는 것이 가장 효과적일 것으로 사료된다. 본 연구의 결과는 감국을 기능성 신소재 개발 원료로 활용하는데 기초 자료가 될 것으로 기대된다.

Abstract ▼ AI-Helper

In the present study, antioxidant and anti-inflammatory activities were measured in Chrysanthemum indicum Linne extracted with different ethanol concentrations. The 50% ethanol extract demonstrated the highest yield of 33.6%, while the 30% and 70% ethanol extracts showed the highest total polyphenol contents of 59.70 and 61.35 mg GAE/g, respectively. The antioxidant activity of 70% ethanol extract was highest in the DPPH radical scavenging activity (RC50 14.95 ㎍/mL) and ABTS+ radical scavenging activity (RC50 42.28 ㎍/mL). FRAP activity was significantly higher in the 30% ethanol extract than the other extracts. The anti-inflammatory effects of Chrysanthemum indicum Linne extracted with different ethanol concentrations were examined using nitric oxide (NO) inhibition assays. In LPS-induced RAW 264.7 cells, the 30% ethanol extract showed the highest inhibition of NO production with 11.16 µM at a concentration of 200 ㎍/mL. The results of this study suggest that Chrysanthemum indicum extracted 30% or 70% ethanol concentrations as potential functional raw material.

주제어

표/그림 (6)

표 Table 1. Extraction yields, total polyphenol, and total flavonoid content of the Chrysanthemum indicum obtained according to different ethanol concentrations
표 Table 2. DPPH radical and ABTS⁺ radicl scavenging activity of Chrysanthemum indicum extracted by different ethanol concentrations
표 Table 3. Reducing power of Chrysanthemum indicum extracted by different ethanol concentrations
그림 Fig. 1. Effect of Chrysanthemum indicum extracted by different ethanol concentrations on RAW 264.7 cells viability. RAW 264.7 cells were treated with various concentrations of extract solutions for 24 h and cell viability was determined using the MTT assay. Data are mean±standard deviation (n=3). Different superscripts in a column indicate significant differences at p<0.05 by Duncan's multiple range test.
그림 Fig. 2. Effect of Chrysanthemum indicum extracted by different ethanol concentrations on LPS-induced nitric oxide production in RAW 264.7 cells. RAW 264.7 cells were treated with different concentrations of each sample and LPS (100 ng/mL) for 24 h. And the NO contents in supernatant was measured using Griess reagent. Data are mean±standard deviation (n=3). Different superscripts in a column indicate significant differences at p<0.05 by Duncan's multiple range test.
그림 Fig. 3. Effect of Chrysanthemum indicum extracted by different ethanol concentrations on LPS-induced iNOS expression in RAW 264.7 cells. RAW 264.7 cells were treated with each sample and LPS (100 ng/mL) for 24 h. Whole cell lysates were analyzed by western blot analysis using β-actin as a loading control.

참고문헌 (37)

Benzie IF. Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power the FRAP assay. Anal. Biochem. 239: 70-76 (1996)

상세보기
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200 (1958)

상세보기
Cheon MS, Yoon TS, Lee DY, Choi GY, Moon BC, Lee AY, Choo BK, Kim HK. Chrysanthemum indicum Linne extract inhibits the inflammatory response by suppressing NF-B and MAPKs activation in lipopolysaccharide-induced RAW 264.7 macrophages. J. Ethnopharmacol. 122: 473-477 (2009)
Cho YJ. Biological Activity of Extracts from Chrysanthemum Incidicum Linne by Ultrafine Grinding. J. Korean Soc. Food Sci. Nutr. 43: 110-117 (2014)
Duh PD, Du PC, Yen GC. Action of methanolic extract of mung bean hulls as inhibitors of lipid peroxidation and non-lipid oxidative damage. Food Chem. Toxicol. 37(11): 1055-1061 (1999)

상세보기
Fatiha B, Khodir M, Farid D, Tiziri R, Karima B, Sonia O, Mohamed C. Optimization of solvent extraction of antioxidants (phenolic compounds) from Algerian mint (Mentha spicata L.). Phcog. Commn. 2: 72-86 (2012)
Folin O, Denis W. On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12: 239-249 (1912)

상세보기
Geetha S, Sai-Ram M, Mongia SS, Singh V, Ilavazhagan G, Sawhney RC. Evaluation of antioxidant activity of leaf extract of sea buckthorn (Hippophae rhamnoides L.) on chromium (VI) induced oxidative stress in albino rats. J. Ethnopharmacol. 87: 247-251 (2003)

상세보기
Green LC, Wagner DA, Glogowski J. Analysis of nitrate, and nitrate in biological fluids. Anal. Biochem. 126: 131-138 (1982)
Halliwell B. Antioxidants in human health and disease. Annu. Rev. Nutr. 16: 33-50 (1996)

상세보기
Heinrich PC, Behrmann I, Muller-Newen G, Schaper F, Graeve L. Interleukin-6-type cytokine signalling through the gp130/Jak/STAT pathway. Biochem. J. 334: 297-314 (1988)
Higuchi M, Higashi N, Taki H, Osawa T. Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-argininedependent mechanism acts as synergistically as the major cytolytic mechanisms of activated macrophages. J. Immunol. 144: 1425-1431 (1990)
Hong UC. Essential oil compositions Chrysanthemum boreale and Chrysanthemum indicum. J. Korean Soc. Agric. Chem. Biotechnol. 45: 108-113 (2002)
Huang D, Ou B, Prior RI. The chemistry behind antioxidant capacity assays. J. Agric. Food Chem. 53: 1841-1856 (2005)

상세보기
Hyun JM, Jo YJ. Kim YB, Park SM, Yoon KS, Lee NH. Antiinflammatory and anti-oxidative activities of flavonoids extracted from Dendranthema indicum flowers in Jeju island. Korean J. Food Sci. Technol. 36(4): 1259-1267 (2019)
Jang DS, Park KH, Lee JR, Ha TJ, Park YB, Nam SH, Yang MS. Antimicrobial activities of sesquiterpene lactones isolated from Hemisteptia lyrata, Chrysanthemum zawadskii, and Chrysanthemum boreale. J. Korean Soc. Agric. Chem. Biotechnol. 44: 137-142 (1999)
Lee JY. Antioxidant and anti-inflammatory activity of ethanol extract of Malus micromalus Makino in Jeju Island. KSBB J. 24: 327-333 (2009)
Li ZF, Wang ZD, Ji YY, Zhang S, Huang C, Li J, Xia XM. Induction of apoptosis and cell cycle arrest in human HCC MHCC97H cells with Chrysanthemum indicum extract. World J. Gastroentero. 15: 4538-4546 (2009)
Lodovici M, Guglielmi F, Meoni M, Dolara P. Effect of natural phenolic acids on DNA oxidation in vitro. Food Chem. Toxicol. 39: 1205-1210 (2001)

상세보기
Middleton JrE, Kandaswami C. Effects of flavonoids on immune and inflammatory cell functions. Biochem. Pharmacol. 43: 1167-1179 (1992)

상세보기
Moncada S, Palmer RM, Higgs EA. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol. Rev. 43: 109-142 (1991)

상세보기
Nakamura M, Ra JH, Kim JS. Evaluation of biological activity for Dangyuja (Citrus grandis) leaves and investigation of optimal concentrations extracted by alternative ethanol concentrations. J. Plant Biotechnol. 46: 45-55 (2019)
Nam SM, Kang IJ, Shin MH. Anti-diabetic and anti-oxidative activities of extracts from Crataegus pinnatifida. J. East Asian Soc. Diet Life 25: 270-277 (2015)
Nieva Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J. Ethnopharmacol. 71: 109-114 (2000)

상세보기
Park CM, Song YS. Luteolin and luteolin-7-O-glucoside inhibit lipopolysaccharide-induced inflammatory responses through modulation of NF-κB/AP-1/PI3K-Akt signaling cascades in RAW 264.7 cells. Nutr. Res. Pract. 7: 423-429 (2013)
Park JH, Lee JK. The encyclopedia of medicinal plants. Shinilbooks Pub., Seoul, Korea. pp. 8 (2000)
Patthamakanokporn O, Puwastien P, Nitithamyong A, Sirichakwa PP. Changes of antioxidant activity and total phenolic compounds during storage of selected fruits. J. Food Compos. Anal. 21: 241- 248 (2008)

상세보기
Ren, AN, Wang, ZG, Lu ZC, Wang, LW, Wu YL. Study on bacteriostasis and antivirotic of flowers Chrysanthemum indicum. Pharm. Biotechnol. 6: 241-244 (1999)
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231-1237 (1999)

상세보기
Ryu SY, Choi SU, Lee CO, Lee SH, Ahn JW, Zee OP. Antitumor activity of some phenolic components in plants. Arch. Pharm. Res. 17: 42-44 (1994)

원문보기 상세보기
Shin YJ, Jeon JR, Park GS. Physicochemical properties of gamgug (Chrysanthemun indicum L.). J. Korean Soc. Food Sci. Nutr. 33: 146-151 (2004)
Shunying Z, Yang Y, Huaidong Y, Yue Y, Guolin Z. Chemicalcomposition and antimicrobial activity of the essential oils of Chrysanthemum indicum. J. Ethnopharmacol. 96: 151-158 (2005)

상세보기
Soobrattee MA, Neergheen VS, Luximon-Ramma A, Aruoma OI, Bahorun OT. Phenolics as potential antioxidant therapeutic agents: mechanism and actions Mutat. Res. Fundam. Mol. 579: 200-213 (2005)
Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 39: 44-48 (2007)
Willoughby DA. Human arthritis applied to animal models towards a better therapy. Ann. Rheum. Dis. 34: 471-478 (1971)
Yoon OH, Cho JS. Optimization of extraction conditions for hat water extracts from Chrysanthemum indicum L. by response surface methodology. Korean J. Food Cook. Sci. 23: 1-8 (2007)
Yoshikawa M, Morikawa T, Toguchida I, Harima S, Matsuda H. Medicinal flowers. II. Inhibitors of nitric oxide production and absolute stereostructures of five new germacrane-type sesquiterpe sesquiterpenes, kikkanols D, D monoacetate, E, F, and F monoacetate from the flowers of Chrysanthemum indicum L. Chem. Pharm. Bull. 48: 651-656 (2002)

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