[논문]해양 섬모충플랑크톤 정량과 정성분석의 현실적 접근

김영옥; 김선영; 최정민; 김재성

doi:10.7850/jkso.2021.26.3.248

[국내논문] 해양 섬모충플랑크톤 정량과 정성분석의 현실적 접근
Practical Approach for Quantitative and Qualitative Analyses of Marine Ciliate Plankton 원문보기

바다 : 한국해양학회지 = The sea : the journal of the Korean society of oceanography, v.26 no.3, 2021년, pp.248 - 262

김영옥 (한국해양과학기술원 해양생태연구센터) , 김선영 (국립해양생물자원관 생물분류실) , 최정민 (한국해양과학기술원 해양생태연구센터) , 김재성 ((주)워터앤에코바이오)

초록
AI-Helper

섬모충플랑크톤은 크게 두 분류군, 세포가 피갑(lorica)에 에워 쌓여 있는 유종섬모류(tintinnids)와 피갑(lorica)이 없는 소모류(oligotrichs)로 대별하고 있다. 국내에서 해양 섬모충플랑크톤의 분류학적 연구는 비교적 종 동정이 용이한 유종섬모류를 대상으로 먼저 시작되었고, 분류기준인 피갑의 가변성 때문에 분류학적 문제점은 있으나, 현재까지도 주로 피갑의 형태적 특징에 따라 종을 구별하여 생태·생리학적 연구정보로 활용하고 있다. 반면, 소모류의 경우, 세포염색을 통한 섬모열의 정밀한 특징으로 종을 구별하는 어려움으로 인해, 소모류의 분류학적 연구는 2000년 이후에 비로소 국내에 소개되었다. 최근 분석기술의 발달과 더불어 섬모충의 분류체계와 종 정보에도 많은 변화가 생겼고 이를 반영하여 국내 해양에서 출현하는 주요 섬모충플랑크톤 종류가 새롭게 정리되어 도감으로 출간되었다. 섬모충플랑크톤은 동물플랑크톤의 질 높은 먹이원이 되는 동시에 초미소(pico)와 미소(nano)플랑크톤의 포식자로서 해양 미세먹이망에서 중요한 중간 역할을 하고 있다. 이러한 부유생태계에서 섬모충플랑크톤의 역할은 새로운 주제가 아닌, 현재는 해양생태학의 보편적 지식으로 공유되고 있다. 따라서 해양 섬모충플랑크톤의 현장 모니터링 방법과 실험실 분석의 과학적 타당성을 기반으로, 획득된 자료로써 그 신뢰성을 확보함은 국내 섬모충플랑크톤 연구 발전에 크게 일조하리라 기대된다. 섬모충플랑크톤 연구 경험을 바탕으로 유종섬모류 동일종의 피갑변이, 세포염색에 의한 소모류의 정량·정성분석, 유전자 분석결과의 자료비교, 생태학적 정보비교, 섬모충플랑크톤 현장채집 방법을 제안하며, 현실적 수용가능 범위를 설정하여 섬모충플랑크톤 모니터링의 정도관리를 위한 연구자들의 관심을 유도하고자 한다.

Abstract ▼ AI-Helper

Marine planktonic ciliates include two major groups, loricated tintinnids and naked oligotrichs. The study of marine ciliate plankton in Korea began with taxonomic efforts on tintinnids based on the morphology of lorica, a vase-shaped shell. Despite polymorphism in the lorica, it is utilized as a key characteristic in identification of tintinnid species. However, oligotrichs have been studied only recently in Korea due to challenges associated with the observation of ciliary arrangements and the technical development for cell staining. Species diversity and phylogenetic classification of the ciliates have been informed by recent advances in morphological and molecular analyses. Illustrations of the planktonic ciliate in Korea have been published on the basis of taxonomic data of tintinnids and oligotrichs. Planktonic ciliates acting as the major consumers of pico- and nanoplankton as well as the prey of mesozooplankton, has been monitored by spatial and temporal investigations in Korean coastal waters. A practical approach addressing the limitations and potential of marine ciliate studies in Korea is proposed here to improve the data quality of planktonic ciliates, providing an enhanced basis for quality control of ciliate monitoring.

Keyword

표/그림 (10)

그림 Fig. 1. A conceptual diagram for the microbial loop of a microbial food webs in relation to the classical food chain. Symbols for diagram courtesy of the Integration and Application Network (ian.umces.edu/media-library).
그림 Fig. 2. Schematic drawing of Quantitative Protargol Staining (QPS) procedure.
그림 Fig. 3. Variation of optimal smoothing curves on sampling interval days based on daily monitoring data (442 days) of tintinnids in Jangmok Bay from Aug. 2008 to Nov. 2009.
그림 Fig. 4. Diagram of the optimized protargol staining (modified from Ji and Wang, 2018).
그림 Fig. 5. Lorica morphology of aggulutinated (A) and hyaline (B) tintinnids.
그림 Fig. 6. Lateral view of generalized features, illustrating some diagnostic ciliary arrangements of Oligotrichia (A) and Choelotrichia (B) ; CM, collar membranelles; BM, buccal menbranelles; GK, girdle kinety; VK, ventral kinety; SK, somatic kinety.
그림 Fig. 7. Four genera of Tontoniidae in Oligotrichia ; Tontonia (A), Paratontonia (B), Pseudotontonia (C), and Spirotontonia (D).
그림 Fig. 8. Lateral (A-C) and aboral (D&E) views of generalized features, illustrating arrangements of somatic kinety (SK); Rimostobilidium (A), Pelagostrobilidium (B), Strombidinopsis (C), Leegaardiella (D), and Lohmaniella (E).
그림 Fig. 9. Relationships between number of ciliate species (tintinnids and oligotrichs) and ciliate abundances from collected data in Jangmok Bay from July 2006 to June 2007.
표 Table 1. List of seqencing primers

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동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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