[논문]우리나라에서 Lactococcus garvieae에 의한 요로감염의 사례

박상현; 이영현; 여민호; 장경수

doi:10.15324/kjcls.2021.53.3.277

우리나라에서 Lactococcus garvieae에 의한 요로감염의 사례
First Case of Urinary Tract Infection by Lactococcus garvieae in Korea 원문보기

Korean journal of clinical laboratory science : KJCLS = 대한임상검사과학회지, v.53 no.3, 2021년, pp.277 - 283

박상현 (부산가톨릭대학교 임상병리학과) , 이영현 (부산가톨릭대학교 임상병리학과) , 여민호 (부산가톨릭대학교 임상병리학과) , 장경수 (부산가톨릭대학교 임상병리학과)

초록
AI-Helper

Lactococcus속은 조건무산소성 그람양성알균으로 catalase 음성, 비운동성이며 10~40℃에서 배양된다. Lactococcus속은 16개의 종으로 구성되어 있으며, 그 중 Lactococcus garvieae와 Lactococcus lactis가 인간질병에 주로 관여하는 것으로 알려져 있다. 본 연구는 발열, 구토, 옆구리 통증으로 내원한 74세 여자의 소변 배양에서 Lactococcus garvieae가 배양된 사례를 최초 보고한다. 상기 환자는 의료기관에서 시행한 혈액검사, 미생물 배양검사, 컴퓨터단층촬영 등의 검사를 통해 급성 요로감염의 소견을 확인하였다. 신장내과 병동에 입원 후 수액처치 및 에리스로마이신 항생제 치료를 시행하였고 5일 후 환자는 퇴원하였다. 본 증례는 우리나라에서 처음으로 요로감염 환자의 소변에서 L. garvieae가 분리된 사례로서 향후 L. garvieae 감염 환자의 치료에 도움이 될 것이다.

Abstract ▼ AI-Helper

The genus Lactococcus is a gram-positive, catalase-negative, non-motile bacterium. It is a facultative anaerobe and can be cultured at 10~40℃. The genus Lactococcus consists of 16 species, of which Lactococcus garvieae and Lactococcus lactis are known to cause disease in humans. This study reports the first case in which L. garvieae was identified in the urine culture of a 74-year-old woman. The patient confirmed the findings of acute urinary tract infection through blood tests, microbial identification tests, antibiotic susceptibility tests, and computed tomography performed at a hospital. The patient was admitted to the nephrology ward and was treated with IV fluids and erythromycin antibiotics and discharged 5 days later. This is the first case in Korea in which L. garvieae was isolated from the urine of a patient with a urinary tract infection and is expected to be useful in treating patients with L. garvieae infection in the future.

주제어

표/그림 (9)

그림 Figure 1. Abdominal computed tomography (CT) shows patchy low density with poor parenchymal enhancement in the right kidney with perirenal strands.
표 Table 1. Clinical and laboratory characteristics in a patient of this study
그림 Figure 2. Lactococcus garvieae cultured from patient urine. (A) and (B) are photographs of BAP agar from which urine samples are cultured, and alpha hemolytic colonies can be observed. (C) is a Gram-stained photograph showing Gram-positive ovarian cocci that occur in pairs and short chains.
표 Table 2. Biochemical characterization for the identification of L. garvieae
그림 Figure 3. 1.2% agarose gel electrophoresis analysis of 16S rRNA gene amplified from Lactococcus garvieae. Lane M, 100 bp DNA ladder; Lane 1, L. garvieae KCCM 40698; Lane 2, L. garvieae isolated from patient.
그림 Figure 4. Phylogenetic tree showing the relationships of the patient sample to other closely related Lactococcus garvieae. Bars indicate 0.01 substitutions per nucleotide.
표 Table 3. PCR primers and conditions used in this study
그림 Figure 5. 1.2% agarose gel electrophoresis analysis of virulence-related genes amplified from Lactococcus garvieae KCCM 40698 (A) and L. garvieae isolated from patient (B). Lane M: 100 bp DNA ladder. Lane 1∼9: hly1, hly2, hly3, fbp, pva, bsh1, bsh2, folP, gapC, respectively. Seven types of toxic genes (hly1, hly2, hly3, fbp, pva, folP, gapC) were identified in the patient-derived microorganisms.
표 Table 4. Information and interpretive criteria for susceptibility testing for Lactococcus spp.*

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