[논문]근권에 존재하는 Bacillus 속 균주들의 식물 생장 촉진 활성 특성

오가윤; 김지윤; 이송민; 김희숙; 이광희; 이상현; 장정수

doi:10.48022/mbl.2105.05013

근권에 존재하는 Bacillus 속 균주들의 식물 생장 촉진 활성 특성
Plant Growth-Promoting Activity Characteristics of Bacillus Strains in the Rhizosphere 원문보기

Microbiology and biotechnology letters = 한국미생물·생명공학회지, v.49 no.3, 2021년, pp.403 - 412

오가윤 ((주)엔젤 식품연구소) , 김지윤 ((주)엔젤 식품연구소) , 이송민 ((주)엔젤 식품연구소) , 김희숙 ((주)엔젤 식품연구소) , 이광희 ((주)엔젤 식품연구소) , 이상현 (신라대학교 바이오산업학부 제약공학전공) , 장정수 ((주)엔젤 식품연구소)

초록
AI-Helper

본 연구에서는 토양 및 근권에 존재하는 Bacillus 속의 식물 생장 촉진 활성, 식물 병원성 곰팡이의 생장 억제활성, 미네랄 가용화능 및 세포 외 효소활성을 확인해 보고자 하였다. 식물 병원성 곰팡이에 대한 항진균 활성에서 DDP257은 10종의 병원성 곰팡이에서 항진균 활성이 모두 나타났다. 식물 생장 촉진 인자인 indole-3-acetic acid 생성능에서는 ANG20이 70.97 ㎍/ml로 가장 높게 나타났다. 추가적으로 1-aminocyclopropane-1-carboxylate deaminase 생성능 조사에서는 총 10종에서 생성능을 확인하였고, 질소 고정능과 siderophore 생성능 조사에서는 대부분의 분리균주에서 활성을 확인하였다. 이후 분리된 균주에 대하여 phosphate, calcite, zinc과 같은 미네랄 가용화능을 확인하였으며 세포외 효소활성에서도 대부분의 효소에서 활성이 나타났다. 특히 alkaline phosphatase, esterase (C4), acid phosphatase, naphtol-AS-BI-phosphohydrolase에서 선별된 균주 모두 유사한 활성을 보였다. 이는 Bacillus 속이 다양한 유기물과 항생물질 및 세포 외 효소를 분비함으로써 이러한 결과가 나타난 것으로 판단된다. 따라서, 본 연구 결과를 통해 토양의 환경 개선에 기여하는 균주를 활용하여 미생물 제제로써의 가능성을 제시한다.

Abstract ▼ AI-Helper

This study aimed to identify plant growth-promoting activity, phytopathogenic fungi growth inhibitory activity, mineral solubilization ability, and extracellular enzyme activity of the genus Bacillus in soil and the rhizosphere. With regards to antifungal activity against phytopathogenic fungi, DDP257 showed antifungal activity against all 10 pathogenic fungi tested. ANG20 showed the highest ability to produce indole-3-acetic acid, a plant growth-promoting factor (70.97 ㎍/ml). In addition, 10 species were identified to have 1-aminocyclopropane-1-carboxylate deaminase production ability, and most isolates showed nitrogen fixation and siderophore production abilities. Thereafter, the isolated strains' ability to solubilize minerals such as phosphate, calcite, and zinc was identified. With extracellular enzyme activity, the activity appeared in most enzymes. In particular, all the strains showed similar abilities for alkaline phosphatase, esterase (C4), acid phosphatase, and naphtol-AS-BI-phosphohydrolase production. This result was observed because the genus Bacillus secreted various organic substances, antibiotics, and extracellular enzymes. Therefore, through the results of this study, we suggest the possibility of using strains contributing to the improvement of the soil environment as microbial agents.

주제어

표/그림 (6)

표 Table 1. Identification of isolated strains by 16S rRNA sequencing.
표 Table 2. Antifungal activity of isolated strains against phytopathogenic fungi.
표 Table 3. Plant growth-promoting activities of isolated strains.
표 Table 4. Mineral solubilization of isolated strains.¹⁾
표 Table 5. Extracellular enzyme activities of isolated strains.¹⁾
표 Table 6. Biochemical characterization of isolated strains by API ZYM kit.

참고문헌 (40)

Kang SJ, Kim JH, Joo GJ. 2005. Isolation of antagonistic bacteria against Fusarium oxysporum and physicochemical properties of compost mixed with microbial formulation. Korean J. Hort. Sci. Technol. 23: 342-350.
Santoro MV, Cappellari LR, Giordano W, Banchio E. 2015. Plant growth-promoting effects of native Pseudomonas strains on Mentha piperita (peppermint): an in vitro study. Plant Biol. 17: 1218-1226.

상세보기
Ahmad F, Ahmad I, Khan MS. 2008. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol. Res. 163: 173-181.

상세보기
Glick BR, Penrose DM, Li J. 1998. A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria. J. Theor. Biol. 190: 63-68.

상세보기
Osman NI, Yin S. 2018. Isolation and characterization of pea plant (Pisum sativum L.) growth-promoting Rhizobacteria. Afr. J. Microbiol. Res. 12: 820-828.

상세보기
Kumar A, Prakash A, Johri BN. 2011. Bacillus as PGPR in crop ecosystem, pp. 37-59. In Maheshwari, DK (eds.), Bacteria in agrobiology: crop ecosystems. Springer, Berlin Heidelberg.
Li XY, Mao ZC, Wu YX, Ho HH, He YQ. 2015. Comprehensive volatile organic compounds profiling of Bacillus species with biocontrol properties by head space solid phase microextraction with gas chromatography-mass spectrometry. Biocontrol Sci. Technol. 25: 132-143.

상세보기
Khan SA, Hamayun M, Yoon HJ, Kim HY, Suh SJ, Hwang SK, et al. 2008. Plant growth promotion and Penicillium citrinum. BMC Microbiol. 8: 231.

상세보기
Barnawal D, Bharti N, Maji D, Chanotiya C, Kalra A. 2014. ACC deaminase-containing Arthrobacter protophormiae induces NaCl stress tolerance through reduced ACC oxidase activity and ethylene production resulting in improved nodulation and mycorrhization in Pisum sativum. J. Plant Physiol. 171: 884-894.

상세보기
Leveau JHJ, Lindow SE. 2005. Utilization of the plant hormone indole-3-acetic acid for growth by Pseudomonas putida strain 1290. Appl. Environ. Microbiol. 71: 2365-2371.

상세보기
Um YR, Kim BR, Jeong JJ, Chung CM, Lee Y. 2014. Identification of endophytic bacteria in Panax ginseng seeds and their potential for plant growth promotion. Korean J. Med. Crop Sci. 22: 306-312.

원문보기 상세보기
Milagres AMF, Machuca A, Napoleao D. 1999. Detection of siderophore production from several fungi and bacteria by a modification of chrome azurol S (CAS) agar plate assay. J. Microbiol. Methods. 37: 1-6.

상세보기
Pande A, Pandey P, Mehra S, Singh M, Kaushik S. 2017. Phenotypic and genotypic characterization of phosphate solubilizing bacteria and their efficiency on the growth of maize. J. Genet. Eng. Biotechnol. 15: 379-391.

상세보기
Tamilselvi SM, Thiyagarajan C, Uthandi S. 2016. Calcite dissolution by Brevibacterium sp. SOTI06: a futuristic approach for the reclamation of calcareous sodic soils. Front. Plant Sci. 7: 1828.
Ramesh A, Sharma SK, Sharma MP, Yadav N, Joshi OP. 2014. Inoculation of zinc solubilizing Bacillus aryabhattai strains for improved growth, mobilization and biofortification of zinc in soybean and wheat cultivated in Vertisols of central India. Appl. Soil Ecol. 73: 87-96.

상세보기
Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428.

상세보기
Shin PY, Cho SJ. 2011. Cellulase and xylanase activity of compost-promoting bacteria Bacillus sp. SJ21. Korean J. Soil Sci. Fert. 44: 836-840.

원문보기 상세보기
Oh DG, Jang YK, Woo JE, Kim JS, Lee CH. 2016. Metabolomics reveals the effect of garlic on antioxidant- and protease-activities during Cheonggukjang (fermented soybean paste) fermentation. Food Res. Int. 82: 86-94.

상세보기
Seher NU, Ahmad M, Hussain A, Jamil M. 2020. Potential of exopolysaccharides producing-lead tolerant Bacillus strains for improving spinach growth under lead stress. Int. J. Agric. Biol. 24: 1845-1854.
Saran A, Imperato V, Fernandez L, Gkorezis P, d'Haen J, Merini LJ, et al. 2020. Phytostabilization of polluted military soil supported by bioaugmentation with PGP-trace element tolerant bacteria isolated from Helianthus petiolaris. Agron. 10: 204.

상세보기
Jo HW, Tagele SB, Pham HQ, Kim MC, Choi SD, Kim MJ, et al. 2020. Response of soil bacterial community and pepper plant growth to application of Bacillus thuringiensis KNU-07. Agron. 10: 551.

상세보기
Contreras-Perez M, Hernandez-Salmeron J, Rojas-Solis D, Rocha-Granados C, Orozco-Mosqueda M del C, Parra-Cota FI, et al. 2019. Draft genome analysis of the endophyte, Bacillus toyonensis COPE52, a blueberry (Vaccinium spp. var. Biloxi) growth-promoting bacterium. 3 Biotech. 9: 1-6.

상세보기
Kim HS, Kim JY, Lee SM, Park HJ, Lee SH, Jang JS, et al. 2019. Isolation and characterization of various strains of Bacillus sp. having antagonistic effect against phytopathogenic fungi. Microbiol. Biotechnol. Lett. 47: 603-613.

원문보기 상세보기
Spaepen S, Vanderleyden J. 2011. Auxin and plant-microbe interactions. Cold Spring Harb. Perspect. Biol. 3: a001438.
Glick BR. 2014. Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiol. Res. 169: 30-39.

상세보기
Saleem M, Arshad M, Hussain S, Bhatti AS. 2007. Perspective of plant growth promoting rhizobacteria (PGPR) containing ACC deaminase in stress agriculture. J. Ind. Microbiol. Biotechnol. 34: 635-648.

상세보기
Hong SH, Lee MH, Kim JS, Lee EY. 2012. An evaluation of plant growth promoting activities and salt tolerance of rhizobacteria isolated from plants native to coastal sand dunes. Microbiol. Biotechnol. Lett. 40: 261-267.

원문보기 상세보기
Seefeldt LC, Hoffman BM, Dean DR. 2009. Mechanism of Modependent nitrogenase. Annu. Rev. Biochem. 78: 701-722.

상세보기
Li SX, Wang ZH, Stewart BA. 2013. Responses of crop plants to ammonium and nitrate N. Adv. Agron. 118: 205-397.

상세보기
Hider RC, Kong X. 2010. Chemistry and biology of siderophores. Nat. Prod. Rep. 27: 637-657.

상세보기
Kamaruzzaman MA, Abdullah SRS, Hasan HA, Hassan M, Othman AR, Idris M. 2020. Characterisation of Pb-resistant plant growth-promoting rhizobacteria (PGPR) from Scirpus grossus. Biocatal. Agric. Biotechnol. 23: 101456.

상세보기
Glick BR. 1995. The enhancement of plant growth by free-living bacteria. Can. J. Microbiol. 41: 109-117.

상세보기
Hameeda B, Harini G, Rupela OP, Wani SP, Reddy G. 2008. Growth promotion of maize by phosphate-solubilizing bacteria isolated from composts and macrofauna. Microbiol. Res. 163: 234-242.

상세보기
Mundra S, Arora R, Stobdan T. 2011. Solubilization of insoluble inorganic phosphates by a novel temperature-, pH-, and salt-tolerant yeast, Rhodotorula sp. PS4, isolated from seabuckthorn rhizosphere, growing in cold desert of Ladakh, India. World J. Microbiol. Biotechnol. 27: 2387-2396.

상세보기
Raddadi N, Cherif A, Boudabous A, Daffonchio D. 2008. Screening of plant growth promoting traits of Bacillus thuringiensis. Ann. Microbiol. 5: 47-52.
Kamran S, Shahid I, Baig DN, Rizwan M, Malik KA, Mehnaz S. 2017. Contribution of zinc solubilizing bacteria in growth promotion and zinc content of wheat. Front. Microbiol. 8: 2593.

상세보기
Mumtaz MZ, Ahmad M, Jamil M, Hussain T. 2017. Zinc solubilizing Bacillus spp. potential candidates for biofortification in maize. Microbiol. Res. 202: 51-60.

상세보기
Shi S, Li S, Asim M, Mao J, Xu D, Ullah Z, et al. 2018. The Arabidopsis calcium-dependent protein kinases (CDPKs) and their roles in plant growth regulation and abiotic stress responses. Int. J. Mol. Sci. 19: 1900.

상세보기
Park KH, Park HW, Lee SW, Lee SH, Myung KS, Lee SY, et al. 2016. Isolation and characterization of Bacillus species having antifungal activity against pathogens of ginseng damping off. Korean J. Pestic. Sci. 20: 380-387.

원문보기 상세보기
Kim BY, Ahn JH, Weon HY, Song J, Kim SI, Kim WG. 2012. Isolation and characterization of Bacillus species possessing antifungal activity against ginseng root rot pathogens. Korean J. Pestic. Sci. 16: 357-363.

원문보기 상세보기

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