[논문]유기발광다이오드를 이용한 Photobiomodulation 기반 스킨케어 효과

김홍빈; 정혜정; 진석근; 이병일; 안재성

doi:10.3807/kjop.2021.32.5.235

[국내논문] 유기발광다이오드를 이용한 Photobiomodulation 기반 스킨케어 효과
Photobiomodulation-based Skin-care Effect of Organic Light-emitting Diodes 원문보기

한국광학회지 = Korean journal of optics and photonics, v.32 no.5, 2021년, pp.235 - 243

김홍빈 (한국광기술원 광의료바이오연구센터) , 정혜정 (한국광기술원 광의료바이오연구센터) , 진석근 ((주)머티어리얼사이언스 OLED연구센터) , 이병일 (한국광기술원 광의료바이오연구센터) , 안재성 (한국광기술원 광의료바이오연구센터)

초록
AI-Helper

Photobiomodulation (PBM) 치료법은 특정 파장대역의 광원이 미토콘드리아에서 ATP 생성을 촉진하는 현상을 이용하는 치료법으로서 상처 치유, 염증 감소, 통증 완화 효과가 있는 것으로 알려져 생물 및 의학 분야에서 많은 관심을 받고 있다. PBM 치료법에 대한 연구는 주로 레이저, 발광다이오드(LED)를 광원으로 사용하였고, 유기발광다이오드(OLED)가 가지는 장점에도 불구하고 PBM 치료법에 사용된 사례는 제한적이다. 본 연구에서는 적색(λ = 620 nm), 녹색(λ = 525 nm), 청색(λ = 455 nm) OLED 조명모듈을 사용하여 PBM에 의한 피부관리 효과를 분석하고 LED에 의한 PBM 효과와 비교하였다. OLED 조명모듈의 PBM에 의한 피부미용효과는 적색 OLED 조명모듈에 의한 collagen type 1 합성량 증가, 녹색 OLED 조명모듈에 의한 melanin 합성 억제, 청색 OLED 조명모듈에 의한 nitric oxide 생성 억제를 각각 측정하여 입증되었다.

Abstract ▼ AI-Helper

Photobiomodulation (PBM)-based therapy, which uses a phenomenon in which a light source of a specific wavelength band promotes ATP production in mitochondria, has attracted much attention in the fields of biology and medicine because of its effects on wound healing, inflammation reduction, and pain relief. Research on PBM-based therapy has mainly used lasers and light-emitting diodes (LEDs) as light sources and, despite the advantages of organic light-emitting diodes (OLEDs), there have been only a few cases where OLEDs were used in PBM-based therapy. In this research, the skin-care effect of PBM was analyzed using red (λ = 620 nm), green (λ = 525 nm), and blue (λ = 455 nm) OLED lighting modules, and was compared to the PBM effect of LEDs. We demonstrated the PBM-based skin-care effect of the red, green, blue OLED lighting modules by measuring the increase in the amount of collagen type-1 synthesis, the inhibition of melanin synthesis, and the suppression of nitric oxide generation, respectively.

주제어

표/그림 (7)

그림 Fig. 1. Photograph of the OLED/LED module used in the experiment. (a) Image of blue (λ = 455 nm), green (λ = 525 nm), red (λ = 620 nm) OLED irradiation by wavelengths using jig. (b) Image of blue (λ = 470 nm), green (λ = 525 nm), red (λ = 623 nm) LED irradiation by wavelengths combined with an LED module in the top-illumination column of an inverted microscope. (c) Normalized spectra of OLED and LED light sources.
그림 Fig. 2. Cell viability measurement by OLED/LED irradiation. (a) Cell viability of raw 264.7 after blue OLED (λ = 455 nm), LED (λ = 470 nm) irradiation with different fluences (3.41, 6.82 J/cm²). (b) Cell viability of B16F10 by green OLED (λ = 525 nm), LED (λ = 525 nm) irradiation of different fluences (3.03, 6.06 J/cm²). (c) Cell viability of Human Dermal Fibroblast (HDF) by red OLED (λ = 620 nm), LED (λ = 623 nm) irradiation of different fluences (2.18, 4.36 J/cm²). All groups showed no cytotoxicity compared to the control group. Every assay was performed 3 times and the results are expressed as mean ± SD. One-way ANOVA (Tukey test) was used for comparisons between two means. **P < 0.01 compared to control.
그림 Fig. 3. Inhibition effect of NO production in raw 264.7 cells by blue OLED (λ = 455 nm), LED (λ = 470 nm) irradiation with different fluences (0.284, 0.568, 0.852, 1.7, 3.41 J/cm²). (a) After blue OLED irradiation. (b) After blue LED irradiation. Every assay was performed 3 times and the results are expressed as mean ± SD. One-way ANOVA (Tukey test) was used for comparisons between two means. **P < 0.01 compared to control.
그림 Fig. 4. Inhibitory effect of tyrosinase activity in B16F10 cells by green OLED (λ = 525 nm), LED (λ = 525 nm) irradiation of different fluences (0.252, 0.505, 0.757, 1.51, 3.03 J/cm²). (a) After green OLED irradiation. (b) After green LED irradiation. Every assay was performed 3 times and the results are expressed as mean ± SD. One-way ANOVA (Tukey test) was used for comparisons between two means.
그림 Fig. 5. Melanin content analysis in B16F10 cells by green OLED (λ = 525 nm), LED (λ = 525 nm) irradiation of different fluences (0.252, 0.505, 0.757, 1.51, 3.03 J/cm²). (a) After green OLED irradiation. (b) After green LED irradiation. Every assay was performed 3 times and the results are expressed as mean ± SD. One-way ANOVA (Tukey test) was used for comparisons between two means. ***P < 0.001 compared to control.
그림 Fig. 6. Immunofluorescence staining of collagen type 1 (green) in HDF cells by OLED (λ = 620 nm), LED (λ = 623 nm) irradiation with different fluences (0.182, 0.363, 0.545, 1.09, 2.18 J/cm²). (a) After red OLED irradiation. (b) After red LED irradiation. Cell nuclei stained with DAPI (blue). Nikon Eclipse Ti2 Microscope (Nikon Instruments Inc., Tokyo, Japan), scale bar: 100 μm.
그림 Fig. 7. Effect of PIP production in HDF cells by red OLED (λ = 620 nm), LED (λ = 623 nm) irradiation with different fluences (0.182, 0.363, 0.545, 1.09, 2.18 J/cm²). (a) After red OLED irradiation. (b) After red LED irradiation. Every assay was performed 3 times and the results are expressed as mean ± SD. One-way ANOVA (Tukey test) was used for comparisons between two means. *P < 0.05, **P < 0.01, ***P < 0.001 compared to control.

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