[논문]Eriodictyol Inhibits the Production and Gene Expression of MUC5AC Mucin via the IκBα-NF-κB p65 Signaling Pathway in Airway Epithelial Cells

Yun, Chawon; Lee, Hyun Jae; Lee, Choong Jae

doi:10.4062/biomolther.2021.091

[국내논문] Eriodictyol Inhibits the Production and Gene Expression of MUC5AC Mucin via the IκBα-NF-κB p65 Signaling Pathway in Airway Epithelial Cells 원문보기

Biomolecules & therapeutics, v.29 no.6, 2021년, pp.637 - 642

Yun, Chawon (Department of Pharmacology, School of Medicine, Chungnam National University) , Lee, Hyun Jae (Smith Liberal Arts College and Department of Addiction Science, Graduate School, Sahmyook University) , Lee, Choong Jae (Department of Pharmacology, School of Medicine, Chungnam National University)

Abstract ▼ AI-Helper

In this study, we investigated whether eriodictyol exerts an effect on the production and gene expression of MUC5AC mucin in human pulmonary epithelial NCI-H292 cells. The cells were pretreated with eriodictyol for 30 min and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 h. The effect of eriodictyol on PMA-induced nuclear factor kappa B (NF-κB) signaling pathway was also investigated. Eriodictyol suppressed the MUC5AC mucin production and gene expression induced by PMA via suppression of inhibitory kappa Bα degradation and NF-κB p65 nuclear translocation. These results suggest that eriodictyol inhibits mucin gene expression and production in human airway epithelial cells via regulation of the NF-κB signaling pathway.

Keyword

표/그림 (4)

그림 Fig. 1. Effect of eriodictyol on PMA-induced MUC5AC gene expression in NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of eriodictyol for 30 min and stimulated with PMA (10 ng/mL) for 24 h. Cell lysates were collected for measurement of MUC5AC gene expression using RT-PCR. Three independent experiments were performed, and the representative data are shown. *Significantly different from control (p<0.05). ^†Significantly different from PMA alone (p<0.05). cont, control; E, eriodictyol. Concentration unit is μM.
그림 Fig. 2. Effect of eriodictyol on PMA-induced MUC5AC mucin production in NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of eriodictyol for 30 min and stimulated with PMA (10 ng/mL) for 24 h. Cell lysates were collected for measurement of MUC5AC mucin production via ELISA. Each bar represents the mean ± standard error of the mean of three culture wells compared to the control set at 100%. Three independent experiments were performed, and the representative data are shown. *Significantly different from control (p<0.05). ^†Significantly different from PMA alone (p<0.05). cont, control; E, eriodictyol. Concentration unit is μM.
$Fig. 3. Effect of eriodictyol on PMA-induced IKK phosphorylation, IκBα phosphorylation, and IκBα degradation in NCI-H292 cells. NCI-H292 cells were incubated with varying concentrations of eriodictyol for 24 h and treated with 50 ng/mL PMA for 30 min. Cytoplasmic extracts were fractionated and then subjected to western blot analysis using phospho-specific IκBα (Ser 32/36) or anti-IκBα antibodies. Whole cell lysates were prepared and then subjected to western blot analysis using phospho-specific IKKα/β (Ser 176/180) antibodies. Equal protein loading was evaluated via β-actin levels. *Significantly different from control (p<0.05). <sup>†</sup>Significantly different from PMA alone (p<0.05). cont, control; E, eriodictyol; IκBα, inhibitory kappa B alpha; IKK, inhibitory kappa B kinase. Concentration unit is μM.$ 그림 Fig. 3. Effect of eriodictyol on PMA-induced IKK phosphorylation, IκBα phosphorylation, and IκBα degradation in NCI-H292 cells. NCI-H292 cells were incubated with varying concentrations of eriodictyol for 24 h and treated with 50 ng/mL PMA for 30 min. Cytoplasmic extracts were fractionated and then subjected to western blot analysis using phospho-specific IκBα (Ser 32/36) or anti-IκBα antibodies. Whole cell lysates were prepared and then subjected to western blot analysis using phospho-specific IKKα/β (Ser 176/180) antibodies. Equal protein loading was evaluated via β-actin levels. *Significantly different from control (p<0.05). ^†Significantly different from PMA alone (p<0.05). cont, control; E, eriodictyol; IκBα, inhibitory kappa B alpha; IKK, inhibitory kappa B kinase. Concentration unit is μM.
그림 Fig. 4. Effect of eriodictyol on PMA-induced NF-κB p65 phosphorylation and translocation in NCI-H292 cells. Nuclear protein extracts were prepared and subjected to western blot analysis using phospho-specific p65 (Ser 536) antibodies and antibodies against p65. p84 was used as a loading control. The results shown are the representative of three independent experiments. *Significantly different from control (p<0.05). ^†Significantly different from PMA alone (p<0.05). cont, control; E, eriodictyol. Concentration unit is μM.

참고문헌 (28)

Adler K. B. Li Y. 2001 Airway epithelium and mucus: intracellular signaling pathways for gene expression and secretion Am. J. Respir. Cell Mol. Biol. 25 397 400 10.1165/ajrcmb.25.4.f214 11694442

상세보기
Choi B. S. Kim Y. J. Choi J. S. Lee H. J. Lee C. J. 2019 Obtusifolin isolated from the seeds of Cassia obtusifolia regulates the gene expression and production of MUC5AC mucin in airway epithelial cells via affecting NF-κB pathway Phytother. Res. 33 919 928 10.1002/ptr.6284 30632219

상세보기
Deshpande R. R. Tiwari A. P. Nyayanit N. Modak M. 2020 In silico molecular docking analysis for repurposing therapeutics against multiple proteins from SARS-CoV-2 Eur. J. Pharmacol. 886 173430 10.1016/j.ejphar.2020.173430 32758569

상세보기
Eniafe G. O. Metibemu D. S. Omotuyi O. I. Ogunleye A. J. Inyang O. K. Adelakun N. S. Adeniran Y. O. Adewumi B. Enejoh O. A. Osunmuyiwa J. O. Shodehinde S. A. Oyeneyin O. E. 2018 Agemone mexicana flavanones; apposite inverse agonists of the β2-adrenergic receptor in asthma treatment Bioinformation 14 60 67 10.6026/97320630014060 29618901

상세보기
Fujisawa T. Velichko S. Thai P. Hung L. Y. Huang F. Wu R. 2009 Regulation of airway MUC5AC expression by IL-1beta and IL-17A; the NF-kappa B paradigm J. Immunol. 183 6236 6243 10.4049/jimmunol.0900614 19841186

상세보기
Garvin L. M. Chen Y. Damsker J. M. Rose M. C. 2016 A novel dissociative steroid VBP15 reduces MUC5AC gene expression in airway epithelial cells but lacks the GRE mediated transcriptional properties of dexamethasone Pulm. Pharmacol. Ther. 38 17 26 10.1016/j.pupt.2016.04.004 27133900

상세보기
Huang W. C. Wu L. Y. Hu S. Wu S. J. 2018 Spilanthol inhibits COX-2 and ICAM-1 expression via suppression of NF-kappaB and MAPK signaling in interleukin-1beta-stimulated human lung epithelial cells Inflammation 41 1934 1944 10.1007/s10753-018-0837-0 29959625

상세보기
Ishinaga H. Takeuchi K. Kishioka C. Suzuki S. Basbaum C. Majima Y. 2005 Pranlukast inhibits NF-kappaB activation and MUC2 gene expression in cultured human epithelial cells Pharmacology 73 89 96 10.1159/000081294 15475658

상세보기
Islam A. Islam M. S. Rahman M. K. Uddin M. N. Akanda M. R. 2020 The pharmacological and biological roles of eriodictyol Arch. Pharm. Res. 43 582 592 10.1007/s12272-020-01243-0 32594426

상세보기
Kim J. O. Sikder M. A. Lee H. J. Rahman M. Kim J. H. Chang G. T. Lee C. J. 2012 Phorbol ester or epidermal growth factor-induced MUC5AC mucin gene expression and production from airway epithelial cells are inhibited by eriodictyol and wogonin Phytother. Res. 26 1784 1788 10.1002/ptr.4650 22388989

상세보기
Kurakula K. Hamers A. A. van Loenen P. de Vries C. J. 2015 6-Mercaptopurine reduces cytokine and Muc5ac expression involving inhibition of NF-kB activation in airway epithelial cells Respir. Res. 16 73 10.1186/s12931-015-0236-0 26084512

상세보기
Laos S. Baeckstrom D. Hansson G. C. 2006 Inhibition of NF-kappaB activation and chemokine expression by the leukocyte glycoprotein, CD43, in colon cancer cells Int. J. Oncol. 28 695 704 10.3892/ijo.28.3.695 16465375

상세보기
Lee J. W. Ryu H. W. Lee S. U. Kim M. G. Kwon O. K. Kim M. O. Oh T. K. Lee J. K. Kim T. Y. Lee S. W. Choi S. Li W. Y. Ahn K. S. Oh S. R. 2019 Pistacia weinmannifolia ameliorates cigarette smoke and lipopolysaccharide-induced pulmonary inflammation by inhibiting interleukin-8 production and NF-kappaB activation Int. J. Mol. Med. 44 949 959 10.3892/ijmm.2019.4247 31257455
Li J. D. Dohrman A. F. Gallup M. Miyata S. Gum J. R. Kim Y. S. Nadel J. A. Prince A. Basbaum C. B. 1997 Transcriptional activation of mucin by Pseudomonas aeruginosa lipopolysaccharide in the pathogenesis of cystic fibrosis lung disease Proc. Natl. Acad. Sci. U.S.A. 94 967 972 10.1073/pnas.94.3.967 9023366

상세보기
Li X. Jin F. Lee H. J. Lee C. J. 2020 Recent advances in the development of novel drug candidates for regulating the secretion of pulmonary mucus Biomol. Ther. (Seoul) 28 293 301 10.4062/biomolther.2020.002 32133827

원문보기 상세보기
Lillehoj E. R. Kim K. C. 2002 Airway mucus: its components and function Arch. Pharm. Res. 25 770 780 10.1007/BF02976990 12510824

원문보기 상세보기
Liu Y. Zhang B. Zhang T. Wang H. Peng L. Zhou L. 2020 Effect of NF-kappaB signal pathway on mucus secretion induced by atmospheric PM 2.5 in asthmatic rats Ecotoxicol. Environ. Saf. 190 10094 10.1016/j.ecoenv.2019.110094 31869718

상세보기
Nie Y. C. Wu H. Li P. B. Xie L. M. Luo Y. L. Shen J. G. Su W. W. 2012 Naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IkappaB-NF-kappaB signaling pathways Eur. J. Pharmacol. 690 207 213 10.1016/j.ejphar.2012.06.040 22766066

상세보기
Rose M. C. Voynow J. A. 2006 Respiratory tract mucin genes and mucin glycoproteins in health and disease Physiol. Rev. 86 245 278 10.1152/physrev.00010.2005 16371599

상세보기
Seo H. S. Sikder M. A. Lee H. J. Ryu J. Lee C. J. 2014 Apigenin inhibits tumor necrosis factor-α-induced production and gene expression of mucin through regulating nuclear factor-kappa B signaling pathway in airway epithelial cells Biomol. Ther. (Seoul) 22 525 531 10.4062/biomolther.2014.094 25489420

원문보기 상세보기
Shang J. Liu W. Yin C. Chu H. Zhang M. 2019 Cucurbitacin E ameliorates lipopolysaccharide-evoked injury, inflammation and MUC5AC expression in bronchial epithelial cells by restraining the HMGB1-TLR4-NF-kappaB signaling Mol. Immunol. 114 571 577 10.1016/j.molimm.2019.09.008 31525576

상세보기
Shao M. X. Ueki I. F. Nadel J. A. 2003 TNF-alpha converting enzyme mediated MUC5AC mucin expression in cultured human airway epithelial cells Proc. Natl. Acad. Sci. U.S.A. 100 11618 11623 10.1073/pnas.1534804100 12972643

상세보기
Takeyama K. Dabbagh K. Lee H. Agusti C. Lausier J. A. Ueki I. F. Grattan K. M. Nadel J. A. 1999 Epidermal growth factor system regulates mucin production in airways Proc. Natl. Acad. Sci. U.S.A. 96 3081 3086 10.1073/pnas.96.6.3081 10077640

상세보기
Wang X. Deng R. Dong J. Huang L. Li J. Zhang B. 2020 Eriodictyol ameliorates lipopolysaccharide-induced acute lung injury by suppressing the inflammatory COX-2/NLRP3/NF-kappaB pathway in mice J. Biochem. Mol. Toxicol. 34 e22434 10.1002/jbt.22434 31860763

상세보기
Wang Y. Chen Y. Chen Y. Zhou B. Shan X. Yang G. 2018 Eriodictyol inhibits IL-1 beta-induced inflammatory response in human osteoarthritis chondrocytes Biomed. Pharmacother. 107 1128 1134 10.1016/j.biopha.2018.08.103 30257325

상세보기
Wu D. Y. Wu R. Reddy S. P. Lee Y. C. Chang M. M. 2007 Distinctive epidermal growth factor receptor/extracellular regulated kinase-independent and -dependent signaling pathways in the induction of airway mucin 5B and mucin 5AC expression by phorbol 12-myristate 13-acetate Am. J. Pathol. 170 20 32 10.2353/ajpath.2007.060452 17200179

상세보기
Xuewen H. Ping O. Zhongwei Y. Zhongqiong Y. Hualin F. Juchun L. Changliang H. Gang S. Zhixiang Y. Xu S. Yuanfeng Z. Lixia L. Lizi Y. 2018 Eriodictyol protects against Staphylococcus aureus-induced lung cell injury by inhibiting alpha-hemolysin expression World J. Microbiol. Biotechnol. 34 64 10.1007/s11274-018-2446-3 29671126

상세보기
Zhang Y. Zhang R. Ni H. 2019 Eriodictyol exerts potent anticancer activity against A549 human lung cancer cell line by inducing mitochondrial-mediated apoptosis, G2/M cell cycle arrest and inhibition of m-TOR/PI3K/Akt signalling pathway Arch. Med. Sci. 16 446 452 10.5114/aoms.2019.85152 32190156

상세보기

LOADING...

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증