[논문]초석잠 추출물의 항산화, 항균 및 항염 활성

이정호

doi:10.7732/kjpr.2021.34.5.420

초석잠 추출물의 항산화, 항균 및 항염 활성
Antioxidant, Antibacterial and Anti-inflammatory Effects of Stachys sieboldii Extract 원문보기

韓國資源植物學會誌 = Korean journal of plant resources, v.34 no.5, 2021년, pp.420 - 432

이정호 ((재)순창건강장수연구소)

초록
AI-Helper

순창군에서 재배한 초석잠을 건강식품을 개발하기 위한 기초자료를 확보하고자 초석잠 물 추출물과 60% 에탄올 추출물에 대한 항산화, 항균, 항염, 소화효소 활성을 측정하였다. DPPH 라디칼 소거능(IC₅₀)은 SAW는 5.26 ± 0.05 mg/mL, SAE는 4.34 ± 0.04 mg/mL로 나타났으며, ABTS 라디칼 소거능(IC₅₀)은 SAW는 6.44 ± 0.06 mg/mL, SAE는 5.05 ± 0.06 mg/mL로 나타났다. 총 폴리페놀 함량은 SAW는 106.25 ± 0.94 mgGAE/g, SAE는 124.61 ± 1.11 mgGAE/g, 총플라보노이드 함량은 SAW는 24.4 ± 0.24 mgQE/g, SAE는 45.2 ± 3.52 mgQE/g으로 분석되었다. CAA assay를 활용한 HepG2 세포내 항산화 활성은 400 ㎍/mL의 농도에서 SAW는 53.2 ± 1.8%, SAE는 54.1 ± 0.4%로 감소되었다. SAW의 MIC는 L. monocytogenes은 100 mg/mL, S. typhimurium와 H. pylori은 125 mg/mL로 측정되었으며, MBC는 L. monocytogenes와 S. typhimurium은 325 mg/mL, H. pylori은 400 mg/mL로 측정되었다. RAW 264.7 세포에서 각 추출물 모두100 ㎍/mL 이하의 농도에서 독성이 나타나지 않았으며, 추출물 100 ㎍/mL 농도에서 SAW는 44.3 ± 1.4%, SAE는 45.1 ± 1.0%로 NO 생성을 저하시켰다. 염증성 사이토카인인 TNF-𝛼, IL-1𝛽 및 IL-6 생성을 농도 의존적으로 억제시켰다. Caco-2 세포에서 SAW와 SAE 추출물 모두 독성이 나타나지 않았으며, 농도 의존적으로 NO 생성을 억제시켰다. 𝛼-Amylase와 protease 효소활성은 초석잠 추출물의 처리 농도가 증가함에 따라 효소의 활성도 증가하였다.

Abstract ▼ AI-Helper

This study was conducted to evaluate antioxidant, antibacterial, anti-inflammatory, and digestive enzyme activity in water extract (SAW) and 60% ethanol extract (SAE) from Stachys sieboldii. As the treatment concentration of each extract S. sieboldii extract increased, antibacterial and antioxidant activity increased. The total polyphenol and total flavonoid contents of SAW were 106.25 ± 0.94 mgGAE/g, 24.4 ± 0.24 mgQE/g and SAE were 124.61 ± 1.11 mgGAE/g, 45.2 ± 3.52 mgQE/g, respectively. The 400 ㎍/mL of SAW and SAE performed more than 53% protective effects against oxidative stress in HepG2 cell lines. All extracts were not showed cytotoxicity to RAW 264.7 cell line at 100 ㎍/mL. NO production was reduced to 44.3 ± 1.4% for SAW and 45.1 ± 1.0% for SAE at a concentration of 100 ㎍/mL. The production of inflammatory cytokines each TNF-α, IL-1β, and IL-6 was inhibited in a concentration-dependent. S. sieboldii extract did not showed Caco-2 cells cytotoxicity and inhibited NO production in concentration-dependent. As the concentration of the S. sieboldii extract increased of α-amylase and protease enzymes activity, which are digestive enzyme. As a result of the experiment, it is judged that it can be used as basic data for the development of health food using S. sieboldii.

주제어

표/그림 (11)

표 Table 1. Measurement of IC₅₀ value for antioxidant activity of Stachys sieboldii extract
표 Table 2. Total polyphenol and flavonoid content of Stachys sieboldii extract
표 Table 3. Cellular antioxidant activity of Stachys sieboldii extracted with water and 60% ethanol against oxidative stress induced by AAPH in HepG2 cell model
표 Table 4. MTT assay on RAW 264.7 cell culture incubated for 24 h with Stachys sieboldii extract
표 Table 5. Nitric oxide (NO) production inhibitory activity (%) on LPS-stimulated RAW 264.7 cell culture incubated for 24 h with Stachys sieboldii extract
표 Table 6. MTT assay on Caco-2 cell culture incubated for 24 h with Stachys sieboldii extract
표 Table 7. Nitric oxide (NO) production inhibitory activity (%) on LPS-stimulated Caco-2 cell culture incubated for 24 h with Stachys sieboldii extract
표 Table 8. Pro-inflammatory cytokines (TNF-α, IL-6, IL-1 β) production on LPS-stimulated RAW 264.7 cell culture incubated for 24 h with Stachys sieboldii extract
표 Table 9. MIC and MBC of Stachys sieboldii extract against L. monocytogenes, S. typhimurium and H. pylori
표 Table 10. Evaluation of digestive enzyme activity through α-amylase activity of Stachys sieboldii extract
표 Table 11. Evaluation of digestive enzyme activity through α-amylase and protease activity of Stachys sieboldii extract

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