[논문]사료 내 갈색거저리(Tenebrio molitor) 유충과 동애등에(Hermetia illucens) 유충의 첨가에 따른 흰다리새우(Litopenaeus vannamei)의 비특이적 면역력, 항산화력, Vibrio parahaemolyticus에 대한 저항성 및 성장 효과

신재형; 신재범; 엄건호; 이경준

doi:10.5657/kfas.2021.0624

사료 내 갈색거저리(Tenebrio molitor) 유충과 동애등에(Hermetia illucens) 유충의 첨가에 따른 흰다리새우(Litopenaeus vannamei)의 비특이적 면역력, 항산화력, Vibrio parahaemolyticus에 대한 저항성 및 성장 효과
Effects of Dietary Mealworm Tenebrio molitor Larvae and Black Soldier Fly Hermetia illucens Larvae on Pacific White Shrimp Litopenaeus vannamei: Innate Immune Responses, Anti-oxidant Enzyme Activity, Disease Resistance against Vibrio parahaemolyticus and Growth 원문보기

한국수산과학회지 = Korean journal of fisheries and aquatic sciences, v.54 no.5, 2021년, pp.624 - 633

신재형 (제주대학교 해양생명과학과) , 신재범 (제주대학교 해양생명과학과) , 엄건호 (제주대학교 해양생명과학과) , 이경준 (제주대학교 해양과학연구소)

Abstract ▼ AI-Helper

This study was conducted to determine the supplemental effects of two insect meals, mealworm (MW) and black soldier fly (BSF), with high or low lipid levels in diets, on Pacific white shrimp Litopenaeus vannamei. Sardine and tuna by-product meals were used as the fish meal source in a control (Con) diet. The fish meals were replaced with MW, defatted MW (deMW), BSF or defatted BSF (deBSF), respectively. The shrimp (body weight: 0.47 g) were stocked into 20 acryl tanks (215 L) and fed the diets six times a day. After 45 days of the feeding trial, the shrimp that were fed insect meals had significantly higher phenoloxidase and superoxide dismutase activities than the shrimp fed Con diet. The gene expressions of prophenoloxidase, crustin and penaeidine-3c in shrimp hepatopancrease were also higher in shrimp that were fed the insect diets, regardless of defatting than those in shirmp that were fed Con diet. The survival against Vibrio parahaemolyticus was higher in shrimp that were fed the diets containing defatted insect meals than in shrimp that were fed Con diet. These results indicate that MW and BSF, regardless of lipid levels, could be good protein sources for the enhancement of innate immunity and anti-oxidant capacity of the shrimp.

주제어

표/그림 (8)

표 Table 1. Nutrient composition of the insect meals and fish meals for Pacific white shrimp Litopenaeus vannamei
표 Table 2. Formulation and proximate composition (%, dry matter) of the experimental diets for Pacific white shrimp Litopenaeus vannamei
표 Table 3. The cycel threshold (Ct) values of hepatopancrease for Photorhabdus insect-related (PirA) toxin-like gene levels in Pacific white shrimp Litopenaeus vannamei at 0, 12, 96 h after Vibrio parahaemolyticus (2×10⁶ colony forming unit/ml) infection challenge
표 Table 4. Innate immune response and anti-oxidant enzyme activity of Pacific white shrimp Litopenaeus vannamei fed the experimental diets for 45 days
표 Table 5. Growth performance, feed utilization and survival of Pacific white shrimp Litopenaeus vannamei (initial body weight, 0.47±0.03 g) fed the experimental diets for 45 days
그림 Fig. 1. Survival of Pacific white shrimp Litopenaeus vannamei fed the experimental diets for 45 days during the challenge with Vibrio parahaemolyticus. The shrimp were immerged with V. parahaemolyticus suspension containing 2×10⁶ colony forming unit/ml. The water in each tank was exchanged 6 h after immersion challenge. Triplicate groups of shrimp were fed one of the experimental diets three times a day during the challenge period. The experimental diets were prepared by replacing fish meal in the control diet (Con) with mealworm (MW), defatted MW (deMW), black soldier fly (BSF) or defatted BSF (deBSF).
표 Table 6. Proximate composition (%, wet basis) of Pacific white shrimp Litopenaeus vannamei fed the experimental diets for 45 days
표 Table 7. Fatty acid composition (%, lipid) of Pacific white shrimp Litopenaeus vannamei muscle fed the experiment diets for 45 days

AI 본문요약
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제안 방법

01 mg/L로 유지되었다. 실험사료는 새우 체중의 4-8%를 1일 6회(08:30, 10:30, 12:30, 14:30, 16:30, 18:30)에 걸쳐 총 45일간 공급되었다. 실험수조의 광주기는 형광등을 통해 조절되었고, 사육수는 수질을 고려하여 2-3일 간격으로 환수하였다.
대조사료(control, Con) 에는 두 종류의 어분(정어리 어분, 참치부산물분)과 대두박 이주요 단백질원으로 사용되었다(Table 2). 실험사료는 총 5종으로 대조사료의 어분단백질을 비탈지 혹은 탈지 갈색거저리와 동애등에 분말로 각각 20% 대체한 4종의 사료(MW, deMW, BSF, deBSF)로 구성하였다. 사료원료는 분쇄기를 이용하여 분쇄한 후 조성표에 따라 혼합되었다.
실험새우(0.47±0.03 g)는 총 20개의 수조(210 L)에 23마리씩 무작위로 사료구 당 4반복으로 배치되었다
실험새우의 최종무게(final body weight, FBW)와 생존율(survival)을 조사하기 위해 새우를 20시간 동안 절식시켰다. 실험새우의 무게와 사료공급량을 이용하여 일간성장률(specific growth ratio, SGR), 단백질이용효율(protein efficiency ratio, PER), 사료계수(feed conversion ratio, FCR)를 계산하였다. 각 실험 수조에서 8마리의 실험새우를 무작위로 선정하여 얼음물로 마취시켰다.

대상 데이터

갈색거저리, 탈지갈색거저리(defatted mealworm, deMW), 동애등에, 탈지동애등에(defatted black soldier fly, deBSF) 가실험에 사용되었다. 갈색거저리 유충 분말은 (주)KEIL (Seoul, Korea)에서, 동애등에 유충 분말은 (주)Entomo (Cheongju, Korea)에서 구매하였다. 원료의 일반성분, 아미노산, 지방산, chitin의 함량은 Table 1에 나타내었다.
갈색거저리, 탈지갈색거저리(defatted mealworm, deMW), 동애등에, 탈지동애등에(defatted black soldier fly, deBSF) 가실험에 사용되었다. 갈색거저리 유충 분말은 (주)KEIL (Seoul, Korea)에서, 동애등에 유충 분말은 (주)Entomo (Cheongju, Korea)에서 구매하였다.

데이터처리

각 항목의 결과는 Duncan’s multiple test를 이용하여 유의성(P<0.05)을 검증하였고, 백분율데이터는 arcsine 변형 값으로 통계분석 하였다.
실험 수조 내 사료의 배치는 완전임의배치법(randomized complete block design)을 이용하였다. 사육실험과 침지 감염실험의 결과는 SPSS (Version 18.0; SPSS, Chicago, IL, USA)를 이용하여 통계 분석되었다(One-way ANOVA). 각 항목의 결과는 Duncan’s multiple test를 이용하여 유의성(P<0.

이론/모형

실험 수조 내 사료의 배치는 완전임의배치법(randomized complete block design)을 이용하였다. 사육실험과 침지 감염실험의 결과는 SPSS (Version 18.

성능/효과

침지감염실험의 경우, V. parahaemolyticus에 대한 새우의 생존율은 두 종의 탈지곤충박 실험구(74.4%)가 대조구(56.4%)와비탈지곤충박 실험구(51.3-53.8%)에 비해 높았다(Fig. 1).
이번 연구에서 사료 내 어분을 곤충박으로 부분 대체할 경우 흰다리새우의 비특이적 면역력, 항산화 효소의 활성, V. parahaemolyticus에 대한 저항성 증진에 도움을 주는 것으로 나타났다. 곤충박을 어분대체원으로 사용할 경우에는 탈지 곤충 박이 비탈지곤충박에 비해 그 이용성이 비교적 높을 것으로 판단된다.
생존율은 실험구 사이에 유의적인 차이를 보이지 않았다. 탈지동애등에구와 일반 동애등에구의 최종평균무게(7.06-7.10 g)와 일간 성장률(6.02-6.03%)은 대조구(6.66 g, 5.89%)에 비해 높은 경향을 보였다. 동애등에 실험구의 사료계수(1.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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