[논문]Puromycin Aminonucleoside에 의해 유도된 신증후군에 대한 옥미수(玉米鬚)의 보호효과

윤정주; 고민철; 한병혁; 김혜윰; 안유미; 이윤정; 이호섭; 강대길

doi:10.15204/jkobgy.2021.34.4.001

Puromycin Aminonucleoside에 의해 유도된 신증후군에 대한 옥미수(玉米鬚)의 보호효과
Renoprotective Effect of Maydis Stigma on Puromycin Aminonucleoside-induced Nephrotic Syndrome 원문보기

大韓韓方婦人科學會誌 = The Journal of Korean obstetrics & gynecology, v.34 no.4, 2021년, pp.1 - 11

윤정주 (원광대학교 한의과대학 생리학교실) , 고민철 (원광대학교 한방심신증후군연구센터) , 한병혁 (원광대학교 한의과대학 생리학교실) , 김혜윰 (원광대학교 한의과대학 생리학교실) , 안유미 (한국한의학연구원 한의과학연구부) , 이윤정 (원광대학교 한의과대학 생리학교실) , 이호섭 (원광대학교 한의과대학 생리학교실) , 강대길 (원광대학교 한의과대학 생리학교실)

Abstract ▼ AI-Helper

Objectives: Nephrotic syndrome is a kidney disorder, which is characterized by proteinuria, edema (swelling), and hyperlipidemia. Maydis Stigma (Corn silk) has been widely used in Asia as a traditional medicine and is known to have a diuretic effect and is used for the treatment of edema and indigestion. Methods: The aim of this study is to investigate the improvement effect of Maydis Stigma in treating nephrotic syndrome induced by puromycin aminonucleoside. Sprague-Dawley rats were intravenously injected with 75 mg/kg/day puromycin aminonucleoside, then treated with either Losartan or 200 mg/kg/day Maydis Stigma for seven days. Results: Maydis Stigma significantly decreased ascites and proteinuria level. Plasma levels of blood urea nitrogen (BUN) and plasma creatinine reduced significantly by Maydis Stigma. In addition, treatment with Maydis Stigma attenuated histological damage. Treatment with Maydis Stigma also restored podocin expression and reduced inflammation markers such as intracellular adhesion molecules (ICAM-1), monocyte chemotactic protein-1 (MCP-1), tumor necrosis factor alpha (TNF-α) and high-mobility group box-1 (HMGB1). Conclusions: Maydis Stigma ameliorates kidney injury in nephrotic syndrome rat models. Maydis Stigma exerts a renoprotective effect owing to its anti-inflammatory effects and reductions of ascites and proteinuria. Thus, these results indicate that Maydis Stigma is likely to be a promising agent in the treatment of nephrotic syndrome.

주제어

표/그림 (6)

표 Table. 1. Effect of Maydis Stigma on renal balance
그림 Fig. 1. Effects of Maydis Stigma on urinary protein excretion at each time point (A) and ascite (B) in PAN-induced nephrotic syndrome model. Rats received PAN (75 mg/kg), intraperitoneally, and then Losartan (30 mg/kg/day) or Maydis Stigma (200 mg/kg/day) was administered. Values were expressed as mean±S.E. (n=7). **p<0.01 versus. Cont., #p<0.05, ##p<0.01 versus PAN. †PAN : puromycin aminonucleoside, ‡PAN+Los. : puromycin aminonucleoside with Losartan, PAN+Maydis Stigma : puromycin aminonucleoside with Maydis Stigma
그림 Fig. 2. Effect of Maydis Stigma on plasma biomarkers in rats with PAN-induced nephrotic syndrome. Values were expressed as mean±S.E. (n=7). **p<0.01 versus. Cont., #p<0.05, ##p<0.01 versus PAN. †PAN : puromycin aminonucleoside, ‡PAN+Los. : puromycin aminonucleoside with Losartan, PAN+Maydis Stigma : puromycin aminonucleoside with Maydis Stigma, BUN : blood urea nitrogen
그림 Fig. 3. Effects of treatment of Maydis Stigma on renal morphology. Representative pictures of PAS (periodic acid-Schiff staining; magnification x200). The bottom panels represent quantitative assessments of protein cast area. The protein cast in the distal tubules is indicated by black arrow in the pictures. Values were expressed as mean±S.E. (n=3). *p<0.05 versus. Cont., #p<0.05 versus PAN. †PAN : puromycin aminonucleoside, ‡PAN+Los. : puromycin aminonucleoside with Losartan, PAN+Maydis Stigma : puromycin aminonucleoside with Maydis Stigma
그림 Fig. 4. Effects of treatment of Maydis Stigma on renal podocin expression. As shown top panels immunohistochemistry staining (A) and western blot (B) in renal tissues (magnification x400). The bottom panels represent quantitative assessments of podocin expression. Values were expressed as mean±S.E. (n=3). *p<0.05 versus. Cont., #p<0.05 versus PAN. †PAN : puromycin aminonucleoside, ‡PAN+Los. : puromycin aminonucleoside with Losartan, PAN+Maydis Stigma : puromycin aminonucleoside with Maydis Stigma
그림 Fig. 5. Effect of Maydis Stigma on the expression of inflammation markers in the renal tissues. The total protein was extracted in kidney tissue and protein levels determined by Western blot analysis. †PAN : puromycin aminonucleoside, ‡PAN+Los. : puromycin aminonucleoside with Losartan, PAN+Maydis Stigma : puromycin aminonucleoside with Maydis Stigma

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