[논문]Effect of insect-resistant genetically engineered (Bt-T) rice and conventional cultivars on the brown planthopper (Nilaparvata lugens Stål)

Sung-Dug, Oh; Eun Ji, Bae; Kijong, Lee; Soo-Yun, Park; Myung-Ho, Lim; Doh-Won, Yun; Seong-Kon, Lee; Gang-Seob, Lee; Soon Ki, Park; Jae Kwang, Kim; Sang Jae, Suh

doi:10.7744/kjoas.20220044

Effect of insect-resistant genetically engineered (Bt-T) rice and conventional cultivars on the brown planthopper (Nilaparvata lugens Stål) 원문보기

Korean journal of agricultural science, v.49 no.3, 2022년, pp.511 - 520

Sung-Dug, Oh (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) , Eun Ji, Bae (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) , Kijong, Lee (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) , Soo-Yun, Park (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) , Myung-Ho, Lim (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) , Doh-Won, Yun (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) , Seong-Kon, Lee (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) , Gang-Seob, Lee (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) , Soon Ki, Park (School of Applied Biosci) , Jae Kwang, Kim , Sang Jae, Suh

Abstract ▼ AI-Helper

Insect-resistant transgenic rice (Bt-T) expresses a toxic protein (mcry1Ac1) derived from the soil bacterium Bacillus thuringiensis found in the rice cultivar Dongjin with an insecticidal property against rice leaf roller (Cnaphalocrocis medinalis). In this study, to investigate the impact of Bt-T on non-target organisms, the feed and oviposition preferences and biological parameters of brown planthopper (Nilaparvata lugens Stål) were comparatively analyzed in four rice cultivars: Dongjin (parent variety), Ilmi (reference cultivar), Chinnong (brown planthopper resistant cultivar) and Bt-T. In the Bt-T and Dongjin cultivars, the feed preferences were 32.4 ± 8.3 and 34.1 ± 6.8%, and the oviposition preferences were 32.5 ± 5.1 and 30.0 ± 5.3% respectively, and there was no statistical significance between these rices. Additionally, in the Bt-T and Dongjin cultivars, the total lifespans from egg to adult were 39.5 ± 6.9 and 40.0 ± 5.8 days, and the weights of adult females were 1.78 ± 0.14 and 1.72 ± 0.16 mg, respectively. Therefore, there was no statistical difference in the biological parameters between these two varieties. Overall, the results indicate that the insect-resistant transgenic rice (Bt-T) did not negatively affect the reproduction and life cycle of brown planthopper, a non-target organism.

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표/그림 (5)

그림 Fig. 1. Feeding and oviposition preference test of brown planthopper (Nilaparvata lugens Stål) to tested rice varieties: Dongjin (parent variety), Ilmi (reference cultivar), Bt-T (insect-resistant genetically engineered rice), and Chinnong (brown planthopper [BPH] resistant cultivar). (A) 24 hours after infestation. (B) 48 hours after infestation. (C) 72 hours after infestation. (D) BPH feeding on rice. (E) Eggs of BPH. Red arrow: BPH.
그림 Fig. 2. Life cycle investigation of brown planthopper (Nilaparvata lugens Stål). Dongjin is parent variety. Bt-T is insect-resistant genetically engineered rice. Ilmi is reference cultivar. Chinnong is brown planthopper (BPH) resistant cultivar. (A) Insect culture for life cycle investigation of BPH. (B) Adult and nymph stage of BPH. a is BPH adult and b is BPH nymph.
표 Table 1. Feeding preference and fecundity of brown planthopper (Nilaparvata lugens Stål) to tested rice varieties.
표 Table 2. Mean developmental period (days ± standard deviation) of brown planthopper (Nilaparvata lugens Stål) reared on Bt rice (Bt-T) and three varieties.
표 Table 3. Fecundity, hatching rate, adult emergence rate and development of brown planthopper (Nilaparvata lugens Stål) reared on Bt rice (Bt-T) and three varieties.

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