[논문]향부자 에틸아세테이트 분획물의 염증 및 가려움증에 관한 연구

김도규; 이지연; 문소현; 김유경; 김나리; 정아름; 장준환; 이재섭; 한상배; 배준태

doi:10.15230/scsk.2022.48.4.303

[국내논문] 향부자 에틸아세테이트 분획물의 염증 및 가려움증에 관한 연구
A Study on Inflammation and Itching of Cyperus rotundus Ethyl Acetate Fractions 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.48 no.4, 2022년, pp.303 - 312

김도규 ((주)제이투케이바이오 기술연구소) , 이지연 ((주)제이투케이바이오 기술연구소) , 문소현 ((주)제이투케이바이오 기술연구소) , 김유경 ((주)제이투케이바이오 기술연구소) , 김나리 ((주)제이투케이바이오 기술연구소) , 정아름 ((주)제이투케이바이오 기술연구소) , 장준환 ((주)제이투케이바이오 기술연구소) , 이재섭 ((주)제이투케이바이오 기술연구소) , 한상배 (충북대학교 약학대학) , 배준태 ((주)제이투케이바이오 기술연구소)

초록
AI-Helper

본 연구에서는 향부자를 이용하여 에틸아세테이트로 분획물을 제조하고, 지표물질 α-cyperone의 함량을 확인하였다. 또한, 향부자 에틸아세테이트 분획물(EAFC) 및 α-cyperone의 항염증 및 가려움증 완화 효능평가를 통하여 기능성 소재로서의 가능성을 확인하였다. EAFC 내의 α-cyperone의 함량을 확인하기 위해 HPLC 분석을 실시한 결과, α-cyperone의 함량이 5.243%임을 확인하였다. EAFC 및 지표성분인 α-cyperone의 염증 완화 효과를 검증하기 위해 lipopolysaccharide (LPS)로 염증반응을 유도시킨 RAW 264.7 대식세포에서의 nitric oxide (NO) 생성 저해능을 확인한 결과, 농도의존적으로 NO 생성을 저해하였다. 추가적인 염증염증 유발 인자인 IL-1β, IL-6, TNF-α, COX-2 및 iNOS의 mRNA 발현 역시 농도의존적으로 저해하였다. EAFC를 함유한 간단한 화장품 제형을 이용하여 임상시험을 실시한 결과, sodium lauryl sulfate (SLS)로 유발된 피부 자극이 진정되는 효과 및 가려움증이 완화되는 효과를 확인하였다. 이를 통하여 향부자는 염증 저해 및 가려움증 완화 효과를 가지는 천연 화장품 원료로 사용 가능할 것으로 사료된다.

Abstract ▼ AI-Helper

In this study, Cyperus rotundus (C. rotundus) was fractionated into ethyl acetate to identify α-cyperone, a representative indicator, and its ethyl acetate fraction were evaluated to confirm the possibilityas a functional cosmetic ingredient. As a result of HPLC analysis, it was confirmed that the content of α-cyperone was 5.243%. In order to verify the inflammatory relief effect of the ethyl acetate fraction from C. rotundus (EAFC) and α-cyperone, it was confirmed that nitric oxide (NO) production inhibitory ability in RAW 264.7 macrophages induced an inflammatory reaction with lipopolysaccharide (LPS). Real-time qPCR analysis confirmed inhibition of mRNA expression level of inflammatory factors, IL-1β, IL-6, TNF-α, COX-2 and iNOS. As a results of conducting a clinical study using a simple cosmetic formulation containing EAFC, it was confirmed that the skin irritation stimulated by sodium lauryl sulfate (SLS) was calming and relieving itching. Through these results, it is believed that the C. rotundus can be used as a natural cosmetic ingredient that has the effect of inhibiting inflammation and relieving itching.

주제어

표/그림 (10)

그림 Figure 1. Chemical structure of α-cyperone.
표 Table 1. Analysis Condition of HPLC
표 Table 2. Primer List of Real-time PCR
$Figure 2. HPLC chromatogram of (A) α-cyperone, and (B) ethyl acetate fraction from C. rotundus.$ 그림 Figure 2. HPLC chromatogram of (A) α-cyperone, and (B) ethyl acetate fraction from C. rotundus.
$Figure 3. Cell viability of RAW264.7 macrophage cell on each concentration of sample ((A): α-cyperone, and (B): ethyl acetate fraction from C. rotundus). Each value presents the mean ± SD of triplicate determinations.$ 그림 Figure 3. Cell viability of RAW264.7 macrophage cell on each concentration of sample ((A): α-cyperone, and (B): ethyl acetate fraction from C. rotundus). Each value presents the mean ± SD of triplicate determinations.
$Figure 4. Nitric oxide production of LPS-stimulated RAW264.7 macrophage cell on each concentration of sample ((A): ethyl acetate fraction from C. rotundus, and (B): α-cyperone). Each value presents the mean ± SD of triplicate determinations. #p < 0.05 indicate a significant difference from sample untreated group, *p < 0.05, **p < 0.01, ***p < 0.001 indicate a significant difference from LPS-treated group.$ 그림 Figure 4. Nitric oxide production of LPS-stimulated RAW264.7 macrophage cell on each concentration of sample ((A): ethyl acetate fraction from C. rotundus, and (B): α-cyperone). Each value presents the mean ± SD of triplicate determinations. ^#p < 0.05 indicate a significant difference from sample untreated group, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 indicate a significant difference from LPS-treated group.
$Figure 5. Relative mRNA levels of IL-1β, IL-6, TNF-α, COX-2, and iNOS in LPS-stimulated RAW264.7 cells on each concentration of ethyl acetate fraction from C. rotundus. Each value presents the mean ± SD of triplicate determinations. #p < 0.05 indicate a significant difference from sample untreated group, *p < 0.05, **p < 0.01, ***p < 0.001 indicate a significant difference from LPS-treated group.$ 그림 Figure 5. Relative mRNA levels of IL-1β, IL-6, TNF-α, COX-2, and iNOS in LPS-stimulated RAW264.7 cells on each concentration of ethyl acetate fraction from C. rotundus. Each value presents the mean ± SD of triplicate determinations. ^#p < 0.05 indicate a significant difference from sample untreated group, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 indicate a significant difference from LPS-treated group.
그림 Figure 6. Relative mRNA levels of IL-1β, IL-6, TNF-α, COX-2, and iNOS in LPS-stimulated RAW264.7 cells on each concentration of α-cyperone. Each value presents the mean ± SD of triplicate determinations. ^#p < 0.05 indicate a significant difference from sample untreated group, ^*p < 0.05, ^**p < 0.01 indicate a significant difference from LPS-treated group.
그림 Figure 7. Clinical study for skin soothing (redness relief) effect on SLS stimulation of EACF. Change of a-value between control and test. ^*p < 0.05 indicate a significant difference between experimental group and a control group. The experiment number of GCCSU is GCC-201130-E12-3217.
그림 Figure 8. Clinical study for Effect of relieving itching on the skin of EACF. Change of a-value between control and test. ^**p < 0.01, ^***p < 0.001 indicate a significant difference between sample treated group and sample untreated group. ^†p < 0.05, ^††p < 0.01, ^†††p < 0.001 indicate a significant difference between experimental group and a control group. The experiment number of GCCSU is GCC-220624-E05-1135.

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