[논문]산화적 스트레스에 대한 천마 추출물의 신경세포 보호 및 항산화 효과

권강범; 김하림; 김예슬; 박은희; 최한별; 류도곤

doi:10.15188/kjopp.2022.12.36.6.209

산화적 스트레스에 대한 천마 추출물의 신경세포 보호 및 항산화 효과
Neuroprotective and Anti-oxidant Effects of Gastrodiae Rhizoma Extracts against Hydrogen Peroxide-induced Cytotoxicity in SH-SY5Y Cells 원문보기

동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, v.36 no.6, 2022년, pp.209 - 212

권강범 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) , 김하림 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) , 김예슬 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) , 박은희 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) , 최한별 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) , 류도곤 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오)

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We recently reported that Gastrodia elata extracts (GEE) had an effects to protect against lipopolysaccharide-induced cognitive impairment in vivo model. In this study, we investigated the neuroprotective effects and the mechanism of action of GEE in hydrogen peroxide (H2O2)-induced cell death of SH-SY5Y human neuroblastoma cell. The SH-SY5Y cells were divided into five groups, including control(non-treated group), 100 μM H2O2, 100, 200, 500 ㎍/㎖ GEE+ 100 μM H2O2 groups. Pre- and co-treatment with GEE prevented cell death induced by 100 μM H2O2 for 24 h in SH-SY5Y cells. Our findings also showed that anti-oxidants enzymes (Cu/Zn superoxide dismutase, Mn superoxide dismutase, catalase) were up-regulated by 100 μM H2O2. But GEE suppressed H2O2-induced anti-oxidants enzymes decrease in a dose-dependent manner. Treatment with GEE also inhibited phosphorylation of eukaryotic initiation factor-2α (eIF-2α) and p38 by H2O2. Taken together, the neuroprotective effects of GEE in terms of recovery of antioxidant enzymes expression, down-regulation of eIF-2α and p38 phosphorylation, and inhibition of cell death are associated with reduced oxidative stress in SH-SY5Y cells.

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표/그림 (5)

그림 Fig. 1. Cytotoxicity test of Gastrodia elata extracts (GEE) in SH-SY5Y c ells. (A) Cells were treated with various concentrations of GEE for 24 h. (B) Cells were pretreated with various concentrations of GEE for 24 h, and then exposed to hydrogen peroxide (H₂O₂) and/or GEE for 24 h. The data are expressed as mean±SD in triplicate. **p<0.01 vs. non-treated group; # p<0.05, ##p<0.01 vs. H₂O₂ treated group.
표 Table 1. Sequences and Accession Numbers for Primer, Forward and Reverse, Used in Real time reverse transcription-polymerase chain rea ction
그림 Fig. 2. Effects of Gastrodia elata extracts (GEE) on SOD1 and SOD2 m RNA, protein expressions and SOD activity in SH-SY5Y cells. Cells wer e pretreated with various concentrations of GEE for 24 h, and then expos ed to hydrogen peroxide(H₂O₂) and/or GEE for 24 h. (A) SOD1 and SOD2 mRNA expressions, (B) SOD1 and SOD2 protein expression, (C) SOD activi ty were analyzed as described in materials and methods. The data are ex pressed as mean±SD in triplicate. **p<0.01 vs. non-treated group; #p<0.0 5, ##p<0.01 vs. H₂O₂ treated group.
그림 Fig. 3. Effects of Gastrodia elata extracts (GEE) on catalase protein ex pression in SH-SY5Y cells. Cells were pretreated with various concentrati ons of GEE for 24 h, and then exposed to hydrogen peroxide(H₂O₂) and/o r GEE for 24 h. Catalase protein expressions were analyzed by western blo ts as described in materials and methods.
그림 Fig. 4. Effects of Gastrodia elata extracts (GEE) on phosphorylated eIF 2α and p38 protein expression in SH-SY5Y cells. Cells were pretreated with various concentrations of GEE for 24 h, and then exposed to hydrog en peroxide(H₂O₂) and/or GEE for 12 h. phosphorylated eIF2α and p38 pr otein expressions were analyzed by western blots as described in material s and methods.

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산화적 스트레스에 대한 천마 추출물의 신경세포 보호 및 항산화 효과
Neuroprotective and Anti-oxidant Effects of Gastrodiae Rhizoma Extracts against Hydrogen Peroxide-induced Cytotoxicity in SH-SY5Y Cells 원문보기

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산화적 스트레스에 대한 천마 추출물의 신경세포 보호 및 항산화 효과 Neuroprotective and Anti-oxidant Effects of Gastrodiae Rhizoma Extracts against Hydrogen Peroxide-induced Cytotoxicity in SH-SY5Y Cells 원문보기

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산화적 스트레스에 대한 천마 추출물의 신경세포 보호 및 항산화 효과
Neuroprotective and Anti-oxidant Effects of Gastrodiae Rhizoma Extracts against Hydrogen Peroxide-induced Cytotoxicity in SH-SY5Y Cells 원문보기

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