[논문]족삼리 양릉천 전침 자극 후 흰쥐 통증 모델에서 microRNA의 차등 발현

김소희; 비슈누몰라칼라 신드후리; 구성태

doi:10.14406/acu.2022.028

족삼리 양릉천 전침 자극 후 흰쥐 통증 모델에서 microRNA의 차등 발현
Differential Expression of microRNAs Following Electroacupuncture Applied to ST36 and GB34 in Rat Models of Chronic Pain 원문보기

Korean journal of acupuncture, v.39 no.4, 2022년, pp.132 - 141

김소희 (부산대학교 건강노화한의과학연구센터) , 비슈누몰라칼라 신드후리 (부산대학교 한의학전문대학원 한의과학과) , 구성태 (부산대학교 한의학전문대학원 한의과학과)

Abstract ▼ AI-Helper

Objectives : Some acupoints are commonly utilized to treat a variety of diseases. The acupoints appear to have a wide range of effects caused by several mechanisms. The purpose of this study is to investigate into the potential role of microRNAs (miRNAs) in the multipotent effects of individual acupoint stimulation. Methods : We examined the miRNA expressions in the dorsal root ganglia (DRG) of neuropathic or inflammatory pain rats following ST36 and GB34 electroacupuncture (EA) stimulation. Neuropathic pain was induced by L5 spinal nerve ligation. Inflammatory pain was induced by knee joint injection of Complete Freund's adjuvant (CFA). EA was given under gaseous anesthesia with the same parameters (1mA, 2Hz, 30 min) in 5 consecutive days. Pain behaviors and miRNA expressions were analyzed. Results : In rats with neuropathic and inflammatory pain, EA treatments significantly enhanced the paw withdrawal threshold and weight-bearing force. After nerve injury, 36 miRNAs were upregulated in the DRG of neuropathic rats, while EA downregulated 10 of them. Furthermore, 14 miRNAs were downregulated following nerve damage, while one was increased by EA. 15 miRNAs were increased in the DRG of inflammatory rats following CFA injection, while 5 were downregulated by EA. Furthermore, 17 miRNAs were downregulated following CFA injection, while 7 were increased by EA. The miRNAs rno-miR-335, rno-miR-381-5p, rno-miR-1306-3p, and rno-miR-1839-3p were regulated by EA in both models. Conclusions : In two pain models, EA applied to ST36 and GB34 regulated miRNA expression differently. There appeared to be both acupoint-specific and non-specific miRNAs, and miRNA regulation of differential protein expression may modulate a variety of EA mechanisms.

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표/그림 (5)

그림 Fig. 1. Effects of EA applied to ST36 and GB34 on paw withdrawal threshold in the rat model of neuropathic pain. It shows the average values (±SEM) of paw withdrawal threshold. Neuropathic pain was induced by tight ligation of L5 spinal nerve. The same procedure without spinal nerve ligation (SNL) was performed into the Sham group. SNL rats were randomly assigned to one of two groups: Acu or Ane. For five days, rats in the Acu group were given EA daily from day 3 to day 7 following ligation. Both the Sham and Ane groups were given gaseous anesthesia with the same time as the Acu group. The paw withdrawal threshold of the Acu animals increased significantly after day five. Symbols indicate values significantly different (p<0.05) from the Ane value by the two-way ANOVA followed by the Dunnett's multiple comparison test. All group are each of 6 animals.
그림 Fig. 2. Effects of EA applied to ST36 and GB34 on weight bearing ratio in the rat model of inflammatory pain. It shows the average values (±SEM) of weight bearing ratio. Inflammatory pain was induced by CFA injection into knee joint. The identical volume of normal saline was administered into the Sham group. CFA-injected rats were randomly assigned to one of two groups: Acu or Ane. For five days, rats in the Acu group were given EA daily from day 2 to day 6 following injection. Both the Sham and Ane groups were given gaseous anesthesia with the same time as the Acu group. The weight bearing ratio of the Acu animals increased significantly after day four. Symbols indicate values significantly different (p<0.05) from the Ane value by the two-way ANOVA followed by the Dunnett's multiple comparison test. All group are each of 6 animals.
그림 Fig. 3. Number of miRNAs regulated by EA in neuropathic pain (A, B) and in inflammatory pain (C, D).
표 Table 1. Down-regulated miRNAs by EA treatment in the rat dorsal root ganglia with neuropathic pain and inflammatory pain (cut-off fold change<1.5)
표 Table 2. Up-regulated miRNAs by EA treatment in the rat dorsal root ganglia with neuropathic pain and inflammatory pain (cut-off fold change<1.5)

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