[논문]백출과 양춘사 추출 혼합물의 항콜레스테롤 효과 및 기전 연구

김하림; 김예슬; 권강범; 정현종

doi:10.15188/kjopp.2022.10.36.5.181

백출과 양춘사 추출 혼합물의 항콜레스테롤 효과 및 기전 연구
Anti-cholesterol Effects and Molecular Mechanism Study of Mixture of Atractylodes Macrocephala and Amomum Villosum Extracts 원문보기

동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, v.36 no.5, 2022년, pp.181 - 186

김하림 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) , 김예슬 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) , 권강범 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) , 정현종 (원광대학교 한의과대학 진단학교실)

Abstract ▼ AI-Helper

Atractylodes macrocephala (AM) and Amomum villosum (AV) are the most common herbs in Korean Medicine to treat digestive diseases. In this study, we investigated the cholesterol lowering effects of mixtures of AM and AV extracts on high cholesterol diet (HCD) induced dyslipidemia mouse model. We classified animals into six different groups; Group 1: Normal diet, Group 2: HCD, Group 3: AV extracts : AM extracts (1:1) (200 mg/kg) + HCD, Group 4: AV extracts : AM extracts (1:2) (200 mg/kg) + HCD, Group 5: AV extracts : AM extracts (1:3) (200 mg/kg) + HCD, Group 6: Simvastatin 40 mg/kg + HCD. After 4 weeks of oral administration of respective drugs, we checked body, liver and epididymal fatweights along with liver and serum triacylglyceride (TG) concentration, total and low density lipoprotein (LDL) cholesterol in serum. Moreover, 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase (HMGCR), LDL receptor (LDLR), and sterol regulatory element-binding protein 2 (SREBP2) were detected by RT PCR or western blot analysis. The overall results showed that mixtures of AM and AV extracts inhibited HCD-induced increases of total cholesterol and LDL cholesterol in serum. Those effects seem to be caused by AM and AV extracts through inhibition of HMGCR expression. And thus blood cholesterol is induced into the liver by increasing LDLR expression, which is regulated by SREBP2 transcrption factor. The cholesterol lowering effects and mechanism of mixtures of AM and AV extracts was similar to the statin. We have identified the potential mixtures of AM and AV extracts as a new treatment for dyslipidemia.

주제어

표/그림 (9)

표 Table 1. High cholesterol diet
표 Table 2. Classification of Experimental Groups
표 Table 3. Sequences and Accession Numbers
그림 Fig. 1. Effects of mixtures of Atractylodes macrocephala extracts (AME) and Amomum villosum extracts (AVE) on body weight of high cholesterol diet-induced mice. CON:control; CD:cholesterol diet; SIM:simvastatin 40 mg/kg. All data present mean±S.D. **p<0.01 vs. control; #p<0.05, ##p<0.01 vs. cholesterol diet. n=10
그림 Fig. 2. Effects of mixtures of Amomum villosum extracts (AVE) and Atractylodes macrocephala extracts (AME) on liver and serum TG concentration. CON:control; CD:cholesterol diet; SIM:simvastatin 40 mg/kg. All data present mean±S.D. *p<0.05; **p<0.01 vs. control; #p<0.05, ##p<0.01 vs. cholesterol diet. n=10
그림 Fig. 3. Effects of mixtures of Amomum villosum extracts (AVE) and Atractylodes macrocephala extracts (AME) on total and LDL cholesterol in serum. CON:control; CD:cholesterol diet; SIM:simvastatin 40 mg/kg. All data present mean±S.D. **p<0.01 vs. control; #p<0.05, ##p<0.01 vs. cholesterol diet. n=10
그림 Fig. 4. Effects of mixtures of Amomum villosum extracts (AVE) and Atractylodes macrocephala extracts (AME) on HMG CoA Reductase (HMGCR) expressions in liver. All data present mean±S.D. CON:control; CD:cholesterol diet; SIM:simvastatin 40 mg/kg. **p<0.01 vs. control; ##p<0.01 vs. cholesterol diet. n=10
그림 Fig. 5. Effects of mixtures of Amomum villosum extracts (AVE) and Atractylodes macrocephala extracts (AME) on cholesterol homeostasis-related genes in liver. CON:control; CD:cholesterol diet; SIM:simvastatin 40 mg/kg. All data present mean±S.D. *p<0.05; **p<0.01 vs. control; #p<0.05 vs. cholesterol diet. n=10
표 Table 4. Effects of mixtures of Amomum villosum extracts (AVE) and Atractylodes macrocephala extracts (AME) on liver and epididymal fat of high cholesterol diet-induced mice.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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