[논문]단삼과 단삼음의 역류성 식도염 억제 효과 및 암세포 이주 억제 효과

권정연; 안상현; 공경환

doi:10.22246/jikm.2022.43.6.1162

[국내논문] 단삼과 단삼음의 역류성 식도염 억제 효과 및 암세포 이주 억제 효과
Inhibitory Effects of Dansam and Dansam-eum on Reflux Esophagitis and Migration of Cancer Cells 원문보기

대한한방내과학회지 = The journal of internal Korean medicine, v.43 no.6, 2022년, pp.1162 - 1185

권정연 (세명대학교 한의과대학 한방내과학교실) , 안상현 (세명대학교 한의과대학 해부학교실) , 공경환 (세명대학교 한의과대학 한방내과학교실)

Abstract ▼ AI-Helper

Purpose: We examined the effects of Dansam (Salvia miltiorrhiza Bunge, SM) and Dansam-eum (DSE) on gastroesophageal reflux disease (GERD) and reflux esophagitis by comparing the inhibitory effects of SM and DSE with the representative treatment of PPI Omeprazole to determine if the effects of the prescription DSE based on Korean medicine are better than those of a single-use of SM. Methods: We performed experiments using both animal models and cancer cells. Results: Comparison of SM and DSE with PPI in the animal model tests revealed that the effects were superior for SM and DSE than for PPI in all categories (8-OHdG, p-IκB, PAR2, COX-1, cathelicidin, p-JNK, Caspase 3, ATP6V1B1, GRPR, serotonin, and NPY). In three categories (COX-1, serotonin, and NPY), SM and DSE showed superior results over the Controls. In the animal model tests, DSE was superior to SM in all categories except for serotonin. The anti-cancer effects observed in cancer cell tests revealed that SM and DSE had meaningful results in terms of cytotoxicity and cell movement rate, as well as in cancer cell apoptosis. Conclusions: We confirmed that SM and DSE can have effects on reflux esophagitis through the regulation of oxidative stress, inflammation, mucosal protection, apoptosis, proton pumping, and the enteroendocrine system in the stomach and esophagus. We also confirmed that SM and DSE have superior effects to those of PPI on all aspects, especially gastric mucosa protection and enteroendocrine system control. We also confirmed that SM and DSE have anti-cancer effects. Above all, we confirmed that DSE has superior effects on almost all aspects compared to using SM alone.

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표/그림 (17)

표 Table 1. Amount and Composition of Dansam-eum (DSE) Extract
그림 Fig. 1. Regulation of oxidative stress in stomach and esophagus by SM and DSE. Ctrl : no GERD elicited group, GERDE : GERD elicited group, OMET : medicine as omeprazole treat group before GERD elicitation, SMeT : medicine as extraction of Salvia miltiorrhiza Bunge treat group before GERD elicitation, DSEeT : medicine as extraction of Dansam-eum treat group before GERD elicitation, Square Box : hemorrhagic erosion, ESO : Esophagus, STO : stomach, MC : Mucosa, LP : laminar propria, SM : sub mucose, Ext. Morph bar size : 1 mm, P/T bar size : 50 μm, Ext. Morph : external Morphology, P/T : Phloxine-Tartrazine stain
표 Table 2. Image Analysis of 8-OHdG in Stomach and Esophagus for Mitigative of GERD by SM and DSE
표 Table 3. Image Analysis of p-IκB, PAR2 in Stomach and Esophagus for Mitigative of GERD by SM and DSE
표 Table 4. Image Analysis of COX-1 in Stomach and Cathelicidin in Esophagus for Mitigative of GERD by SM and DSE
표 Table 5. Image Analysis of p-JNK, Caspase 3 in Stomach and Esophagus for Mitigative of GERD by SM and DSE
표 Table 6. Image Analysis of ATP6V1B1, GRPR in Stomach for Mitigative of GERD by SM and DSE
그림 Fig. 2. Effects of SM and DSE on AGS cells. AGS cells were treated with SM (A) or DSE (B) for 24 h. Cell viability was measured by WST-1 (4-[3- (4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene Disulfonate) assay. These data are expressed as mean percentages relative to the untreated group±standard deviations. * : P<0.05 vs. untreated group.
표 Table 7. Image Analysis of Serotonin, NPY in Esophagus for Mitigative of GERD by SM and DSE
그림 Fig. 3. Effects of SM on scratch wound-healing of AGS cells. (A) The photographs representative the scratch wound healing on stimulated with SM in AGS cells. Cells were treated with SM (100-1000 μg/mL) in DMEM for 24 h, respectively. (0 h) a white lines indicate the initial scratches; (24 h); a white lines indicate the migrated cell position; Magnification×200. (B) Migration rates of AGS cells relative to the untreated group (%). UN : untreated group. Results are presented as mean±standard deviation. * : P<0.05 verse untreated group, # : P<0.05 verse FBS group.
그림 Fig. 4. Effects of DSE on scratch wound-healing of AGS cells. (C) The photographs representative the scratch wound healing on stimulated with DSE (C) in AGS cells. Cells were treated with DSE (100-1000 μg/mL) in DMEM for 24 h, respectively. (0 h) a white lines indicate the initial scratches; (24 h); a white lines indicate the migrated cell position; Magnification×200. (D) Migration rates of AGS cells relative to the untreated group (%). UN : untreated group. Results are presented as mean±standard deviation. * : P<0.05 verse untreated group, # : P<0.05 verse FBS group.
그림 Fig. 5. Effects of SM and DSE on apoptosis in AGS cells. The AGS cells were seeded (1×10⁴ cells/mL) in slide and treated with SM (500 and 1000 μg/mL) or DSE (500 and 1000 μg/mL) for 24 h. (A and C) Cells were fixed using 4% formaldehyde for 5 min. The nuclear stained with Hoechst (excitation : 361, emission : 497) solution for 10 min at room temperature. (B and D) The live cells were incubated with Mito Tracker solution (exitation : 581 nm, emission : 644 nm) for 30 min at 37 ℃. Fluorescence images were obtained by fluorescence microscopy.
그림 Fig. 6. Effects of SM and DSE on HEp-2 cells. HEp-2 cells were treated with SM (A) or DSE (B) for 24 h. Cell viability was measured by WST-1 (4-[3-(4- Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene Disulfonate) assay. These data are expressed as mean percentages relative to the untreated group±standard deviations. * : P<0.05 vs. untreated group.
그림 Fig. 7. Effects of SM on scratch wound-healing of HEp-2 cells. (A) The photographs representative the scratch wound healing on stimulated with SM in HEp-2 cells. Cells were treated with SM (100-1000 μg/mL) in DMEM for 24 h, respectively. (0 h) a white lines indicate the initial scratches; (24 h); a white lines indicate the migrated cell position; Magnification×200. (B) Migration rates of HEp-2 cells relative to the untreated group (%). UN : untreated group. Results are presented as mean±standard deviation. * : P<0.05 verse untreated group, # : P<0.05 verse FBS group.
그림 Fig. 8. Effects of DSE on scratch wound-healing of HEp-2 cells. (C) The photographs representative the scratch wound healing on stimulated with DSE (C) in HEp-2 cells. Cells were treated with DSE (100-1000 μg/mL) in DMEM for 24 h, respectively. (0 h) a white lines indicate the initial scratches; (24 h); a white lines indicate the migrated cell position; Magnification×200. (D) Migration rates of HEp-2 cells relative to the untreated group (%). UN : untreated group. Results are presented as mean±standard deviation. * : P<0.05 verse untreated group, # : P<0.05 verse FBS group.
그림 Fig. 9. Effects of SM and DSE on apoptosis in HEp-2 cells. The HEp-2 cells were seeded (1×10⁴ cells/mL) in slide and treated with SM (1000 and 20000 μg/mL) or DSE (1000 and 20000 μg/mL) for 24 h. (A and C) Cells were fixed using 4% formaldehyde for 5 min. The nuclear stained with Hoechst (excitation : 361, emission : 497) solution for 10 min at room temperature. (B and D) The live cells were incuabted with Mito Tracker solution (exitation : 581 nm, emission : 644 nm) for 30 min at 37 ℃. Fluoresscence images were obatined by fluorescnece microscopy.
그림 Fig. 10. No adverse effects in stomach, esophagus and liver by SM and DSE. Ctrl : no treatment group, OSMeT : medicine as extraction of Salvia miltiorrhiza Bunge treat group, ODSEeT : medicine as extraction of Dansam-eum treat group, bar size : 50 μm.

참고문헌 (64)

Jung HK, Hong SJ, Jo YJ, Jeon SW, Cho YK,？Lee KJ, et al. Updated Guidelines 2012 for？Gastroesophageal Reflux Disease. Korean J？Gastroenterol 2012;60(4):195-218.
Fauci AS, Braunwald E, Kasper DL, Hauser？SL, Longo DL, Jameson JL, et al. Harrison's？Principles of Internal Medicine 17th？edition. Seoul:？MIP; 2010, p. 688-92, 2232-4.
Maton PN. Omeprazole. N Engl J Med 1991;？324(14):965-75.

상세보기
Kim DH, Lee JE, You SH, Lee HA, Park JH,？Jung JH, et al. Proton pump inhibitor use and？risk of osteoporotic fracture in Korean adults？with peptic ulcer disease and gastroesophageal？reflux disease Seoul: National Evidence-based？Healthcare Collaborating Agency; 2017, p. 67-70.
Cheungpasitporn W, Thongprayoon C, Kittanamongkolchai？W, Srivali N, Edmonds PJ, Ungprasert P, et al.？Proton pump inhibitors linked to hypomagnesemia:？a systematic review and meta-analysis of observational？studies. Ren Fail 2015;37(7):1237-41.

상세보기
Park HJ, Park SH, Shim KN, Kim YS, Kim HY,？Han JP, et al. The Prevalence and Clinical？Features of Non-responsive Gastroesophageal？Reflux Disease to Practical Proton Pump Inhibitor？Dose in Korea: A Multicenter Study. Korean J？Gastroenterol 2016;68(1):16-22.
Makunts T, Alpatty S, Lee KC, Atayee RS,？Abagyan R. Atayee & Ruben Abagyan. Proton-pump？inhibitor use is associated with a broad spectrum？of neurological adverse events including impaired？hearing, vision, and memory. Scientific Reports？2019;9(1):17280.
Bezabeh S, Mackey AC, Kluetz P, Jappar D,？Korvick J. Accumulating Evidence for a Drug-Drug？Interaction Between Methotrexate and Proton？Pump Inhibitors. The Oncologist 2012;17(4):550-4.

상세보기
黃度淵. 新增方藥合編. 서울: 永林社; 2010, p.？391.
陳修園. 醫學全書⋅時方歌括. 北京: 中國中醫藥？出版社; 2006, p. 956.
전국한의과대학 본초학 교수 공편저. 본초학.？Seoul: 永林社; 2011, p. 336-7, 404-5, 458-9.
Jeon CM, Min GW, Park JH, Yoon CH, Jeong？JC, Kang JJ, et al. Effect of the methanol？extract of Dansameum on the ammonia-induced？peptic ulcer in rats. Korean J Orient Int Med？2000;21(4):597-604.
Lee SE, Cho SI. Anti-inflammatory effects of？Salviae Miltiorrhizae Radix extract on RAW264.7？cell. via anti-oxidative activities. Kor J Herbol？2015;30(4):89-94.
Shon YH, Cho HJ, Hyeun WC, Son KH, Nam？KS. Chemopreventive Potential of Salvia miltiorrhiza？Fraction Extracts. Journal of Life Science 2006;？16(3):369-74.
Choi SM, Choi SH, Ahn KS. Study on Antitumor？and Apoptosis - Inducing Effects of Salviae？miltiorrhizae Radix. Korean J of Oriental Medical？Pathology 2000;14(2):22-47.
Lee WYW, Cheung CCM, Liu KWK, Fung KP,？Wong J, Lai PBS, et al. Cytotoxic effects of？tanshinones from Salvia miltiorrhiza on doxorubicin-resistant human liver cancer cells. J Nat Prod？2010;73(5):854-9.
Cha JY, Ha SE, Sim SM, Park JK, Chung YO,？Kim HJ, et al. Antimicrobial Effects of Ethanol Extracts of Korea Endemic Herb Plants. Journal？of Life Science 2008;18(2):228-33.
Lee SK, Lim SW. The Administration of？Jeungmiyijin-tang to Rats with Induced Gastro？Reflux Esophagitis. J Int Korean Med 2016;37(6):1030-41.
Lee ST, Kwak MA. Effects of Individual Herbal？Components of Yijintang-gamibang in the Rat？Reflux Esophagitis. Korean J Orient Int Med？2013;34(2):165-77.
Jang MW, Lim SW. Experimental Study for？Effect of Banhasasim-tang on Mice with Reflux？Esophagitis. Korean J Orient Int Med 2013;34(4):362-3.
Shin MK, Kim ES, Kim TR, Lim HC, Lee？YS. Effects of Jwa Kum-Whan on Reflux？Esophagitis in Rats. J Int Korean Med 2016;37(3):495-507.
Lee JA, Park HJ, Kim SH, Kim MJ, Kim KJ,？Shin MR, et al. Evaluation of Evodiae Fructus？Extract on the Chronic Acid Reflux Esophagitis？in Rats. Kor J Herbol 2019;34(2):15-23.
Kim DJ, Roh SS. Effect on Acute reflux？Esophagitis by Evodiae Fructus Aquous Extract.？Kor J Herbol 2012;27(1):51-8.

원문보기 상세보기
Kim SH, Roh SS, Lee JA, Shin MR, Lee AR,？Koo JS, et al. Improving Effects on Rats with？Chronic Acid Reflux Esophagitis Treated of？Coptidis Rhizoma Extract. Kor J Herbol 2019;34(1):117-24.
Lee JY, Seo BI, Roh SS. Improving effect of？Artemisiae Capillaris Herba extract in reflux？esophagitis rats. Kor J Herbol 2016;31(6):37-44.

원문보기 상세보기
Kwon OJ, Lee AR, Roh SS. Improving Effects？on Rats with Reflux Esophagitis Treated with？Combined Extract of Young persimmon fruit？and Citrus peel. Kor J Herbol 2016;31(1):25-31.

원문보기 상세보기
Kim MY, Shin YO, Lee JY, Lee AR, Shin SH,？Kwon OJ, et al. Improving Effect of a Combined？Extract of Rhei Rhizoma and Glycyrrhizae？Rhizoma through Anti-oxidative Stress in Reflux？Esophagitis rats. Kor J Herbol 2015;30(4):37-44.

원문보기 상세보기
Shin MR, Lee JA, Kim MJ, An HJ, Roh SS.？The protective effect of Areca Semen and？Toosendan Fructus mixture in a chronic model？of reflux esophagitis. Kor J Herbol 2020;35(1):57-68.
Song CH, Baek TH. A Comparative Study of？Sepiae Os, Arcae Concha, Ostreae Concha and？Esomeprazole in a Mouse Model of Reflux？Esophagitis. J Korean Med 2018;39(2):92-105.
Shin MH, Kim ES, Lee YS. Suppressive Effects？of Ulmi Pumilae Cortex Extracts on the Reflux？Esophagitis in Rat. J Physiol & Pathol Korean？Med 2016;30(4):257-65.
Choi MJ, Yang WJ, Lee BH, Cho SI. Basic？network pharmacological analysis of Salvia？miltiorrhiza root for further application to an？animal stroke model. Herbal Formula Science？2021;29(1):19-31.
Kim BC, Lee EJ, Park CS, Park CG. The？Experimental Study on the Effects of Dansamyeum？on Hyperlipidemia. Kor J Orient Int Med 2000;21(1):126-34.
Son CS, Kwon KB, Kim SB, Lee HS, Seo EH,？Lee HS, et al. Effects of Dansamyeum Water？Extract on Heart Beating Rate in Cultured Mouse？Myocardial Cells. Korean J Oriental Medical？Physiology & Pathology 2001;15(2):241-5.
Jeon CM, Min GU, Park JH, Yoon CH, Jeong JC,？Kang JJ, et al. Effect of the methanol extract？of Dansameum on the ammonia-induced peptic？ulcer in rats. Kor J Orient Int Med 2000;21(4):597-604.
Shapiro M, Green C, Bautista JM, Dekel R,？Risner-Adler S, Whitacre R, et al. Assessment？of dietary nutrients that influence perception？of intraoesophageal acid reflux events in patients？with gastro-esophageal reflux disease. Aliment？Pharmacol Ther 2007;25(1):93-101.
Nakamura k, Ozawa Y, Furuta Y, Omd Miyazaki？H. Effects of sodiumpolyacrylate (PANa) on？acute esophagitis by gastric juice in rats. Japan？J Pharmacol 1982;32(3):445-56.
Zhou X, Zhuang Z, Wang W, He L, Wu H, Cao？Y, et al. OGG1 is essential in oxidative stress？induced DNA demethylation. Cell Signal 2016;28(9):1163-71.

상세보기
Valavanidis A, Vlachogianni T, Fiotakis C.？8-hydroxy-2'-deoxyguanosine (8-OHdG):A critical？biomarker of oxidative stress and carcinogenesis.？J Environ Sci Health C Environ Carcinog？Ecotoxicol Rev 2009;27(2):120-39.
Lazzerini PE, Lorenzini S, Selvi E, Capecchi PL,？Chindamo D, Bisogno S, et al. Simvastatin？inhibits cytokine production and nuclear factor-κB？activation in interleukin 1β-stimulated synoviocytes？from rheumatoid arthritis patients. Clinical and？Experimental Rheumatology 2007;25(5):696-700.
Li M, Yang X, Zhang Y, Chen L, Lu H, Li X,？et al. Activation of protease-activated receptor-2？is associated with increased expression of？inflammatory factors in the adipose tissues of？obese mice. Mol Med Rep 2015;12(4):6227-34.

상세보기
Ferrell WR, Lockhart JC, Kelso EB, Dunning？L, Plevin R, Meek SE, et al. Essential role for？proteinase-activated receptor-2 in arthritis. J？Clin Invest 2003;111(1):35-41.

상세보기
Cryer B, Dubois A. The advent of highly selective？inhibitors of cyclooxygenase-a review. Prostaglandins？Other Lipid Mediat 1998;56(5-6):341-61.
Laine L, Takeuchi K, Tarnawski A. Gastric？mucosal defense and cytoprotection: bench to？bedside. Gastroenterology 2008;135(1):41-60.

상세보기
Sorenseon O, Cowland JB, Askaa J, Borregaard？N. An ELISA for hCAP-18, the cathelicidin？present in human neutrophils and plasma. J？Immunol Methods 1997;206(1-2):53-9.
Hase K, Eckmann L, Leopard JD, Varki N,？Kagnoff MF. Cell differentiation is a key？determinant of cathelicidin LL-37/human cationic？antimicrobial protein 18 expression by human？colon epithelium. Infection and Immunity 2002;70(2):953-63.

상세보기
Ip YT, Davis RJ. Signal transduction by the？c-Jun N-terminal kinase (JNK) - from inflammation？to development. Current Opinion in Cell Biology？1998;10(2):205-19.
Choi BH, Hur EM, Lee JH, Jun DJ, Kim KT.？Protein kinase C delta-mediated proteasomal？degradation of MAP kinase phosphatase-1？contributes to glutamate-induced neuronal cell？death. J Cell Sci 2005;119(Pt 7):1329-40.
Oh HL, Seok JY, Kwon CH, Kang SK, Kim？YK. Role of MAPK in ceramide-induced cell？death in primary cultured astrocytes from mouse？embryonic brain. NeuroToxicology 2006;27(1):31-8.

상세보기
Chuang SM, Wang IC, Yang JL. Roles of JNK,？p38 and ERK mitogen-activated protein kinases？in the growth inhibition and apoptosis induced？by cadmium. Carcinogenesis 2000;21(7):1423-32.

상세보기
Porter AG, Janicke RU. Emerging roles of caspase-3？in apoptosis. Cell Death & Differentiation 1999;6(2):99-104.

상세보기
Stover EH, Borthwick KJ, Bavalia C, Eady N,？Fritz DM, Rungroj N, et al. Novel ATP6V1B1？and ATP6V0A4 mutations in autosomal recessive？distal renal tubular acidosis with new evidence？for hearing loss. J Med Genet 2002;39(11):796-803.

상세보기
Finberg KE, Wagner CA, Bailey MA, Paunescu？TG, Breton S, Brown D, et al. The B1-subunit？of the H+ ATPase is required for maximal？urinary acidification. PNAS 2005;102(38):13616-21.

상세보기
Czepielewski RS, Porto BN, Rizzo LB, Roesler R,？Abujamra AL, Pinto LG, et al. Gastrin-releasing？peptide receptor (GRPR) mediates chemotaxis？in neutrophils. PNAS 2012;109(2):547-52.

상세보기
Petronilho F, Danielski LG, Roesler R, Schwartsmann？G, Dal-Pizzol F. Gastrin-releasing peptide as a？molecular target for inflammatory diseases: an？update. Inflamm Allergy Drug Targets 2013;12(3):172-7.
Pederson RA, Kwok YN, Coy DH. Gastrin？secretion from human antral G cells in culture.？Gastroenterology 1990;99(1):36-44.

상세보기
Foxx-Orenstein AE, Kuemmerle JF, Grider JR.？Distinct 5-HT receptors mediate the peristaltic？reflex induced by mucosal stimuli in human？and guinea pig intestine. Gastroenterology 1996;111(5):1281-90.

상세보기
Kim DY, Camilleri M. Serotonin: A mediator？of the brain-gut connection. Am J Gastroenterol？2000;95(10):2698-709.
Jin JG, Foxx-Orenstein AE, Grider JR. Propulsion？in guinea pig colon induced by 5-hydroxytryptamine？(HT) via 5-HT4 and 5-HT3 receptors. J Pharmacol？Exp Ther 1998;288(1):93-7.
Keast JR, Furness JB, Costs M. Distribution？of certain peptide YY containing nerve fibers？and endocrine cells in the gastrointestinal mucosa？in five mammalian species. J Comp Neurol 1985;263(3):403-22.
Allen JM, Gu KJ, Adrian TE, Polak JM, Bloom？SR. Neuropeptide Y in the guinea pig biliary？tract. Experientia 1984;40(7):765-7.

상세보기
Park JG, Hyun JW, Lim KW, Shin JE, Won？YJ, Shin KH, et al. Antineoplastic Effect of？Extracts from Traditional Medicinal Plants.？The Korean Society of Pharmacognosy 1993;24(3):223-30.
Nam GH, Jo KJ, Park YS, Kwak HW, Wee JH,？Kim JH, et al. Anti-cancer Effect of Cinnamomum？camphora Ethanol Extract by Double Induction？of Apoptotic and Autophagic Cell Death in？HCT 116 and HT-29 Human Colon Cancer？Cell through the mTOR Signaling Pathway. Korean？Society for Biotechnology and Bioengineering？Journal 2019;34(2):114-9.

상세보기
Jin Z, El-Deiry WS. Overview of cell death signaling？pathways. Cancer Biol Ther 2005;4(2):147-71.
Okada H, Mak TW. Pathways of apoptotic and？non-apoptotic death in tumour cells. Nat Rev？Cancer 2004;4(8):592-603.

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