[논문]홍조류 유래 네오아가로올리고당의 면역 활성 증강 효과

김경운; 원지연; 김은주; 이제현; 이미연; 나득채

doi:10.9799/ksfan.2022.35.1.043

홍조류 유래 네오아가로올리고당의 면역 활성 증강 효과
Immune Enhancing Activity of Neoagarooligosaccharides from Marine Red Algae 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.35 no.1, 2022년, pp.43 - 50

김경운 ((주)온코인사이트) , 원지연 ((주)온코인사이트) , 김은주 (다인바이오(주)) , 이제현 (다인바이오(주)) , 이미연 ((주)온코인사이트) , 나득채 ((주)온코인사이트)

Abstract ▼ AI-Helper

Agar, a heterogeneous polymer of galactose, is the main component of the cell wall of marine red algae. It is well established as a safe, non-digestible carbohydrate in oriental countries. Neoagarooligosaccharides (NAOs) prepared by hydrolyzing agar by microbial β-agarase have been reported to show safety. However, their immunological effects have not been reported yet. Thus, the objective of this study was to investigate immune enhancing effects of neoagarooligosaccharides (NAOs) from marine red algae Gelidium elegans in mice by performing ex vivo experiments. Six-week-old mice were fed ad libitum. NAOs were orally administrated at three different concentrations (100, 500, and 2,500 mg/kg B.W./day) twice a week for four weeks. The group fed with NAOs at 2,500 mg/kg showed the highest proliferation of splenocytes and production levels of cytokines (IL-1β, IL-6, TNF-α) in the ex vivo experiment. In conclusion, NAOs can enhance immune function, increase proliferation of splenocytes, and increase cytokine production by activating macrophages in mice.

주제어

표/그림 (5)

표 Table 1. Conditions for animal breeding
그림 Fig. 1. Effect of orally administrated neoagarooligosaccharides from marine red algae on the splenocyte proliferation. NAOs was orally treated for 4 weeks (twice/week) at the concentration of 100, 500 or 2,500 mg/kg body weight. Isolated splenocytes were stimulated with mitogen (Con A; 5 mg/mL, LPS; 1 mg/mL) for 24hr. Cell proliferation was determined by MTT assay. Results are expressed as the mean±S.D. (n=4). Statistical analysis was performed using student's two tails t-test with a significant level of *p<0.05, **p<0.01 compared to 0 (Control group). Proliferation index=mean of O.D. in test wells/mean of O.D. in control wells. NAOs; Neoagarooligosaccharides.
그림 Fig. 2. IL-1b production by activated peritoneal macrophages of mice orally administrated with neoagarooligosaccharides from marine red algae for 4 weeks. NAOs was orally treated for 4 weeks (twice/week) at the concentration of 100, 500 or 2,500 mg/kg body weight. Isolated peritoneal macrophages were stimulated with mitogen (LPS; 1 mg/mL) for 24 hr. The concentrations of IL-1b were determined by triplicates cultured supernatant cells and values are mean±S.D. (n=4). Statistical analysis was performed using student's two tails t-test with a significant level of *p<0.05, **p<0.01 compared to 0 (Control group). NAOs; Neoagarooligosaccharides.
그림 Fig. 3. IL-6 production by activated peritoneal macrophages of mice orally administrated with neoagarooligosaccharides from marine red algae for 4 weeks. NAOs was orally treated for 4 weeks (twice/week) at the concentration of 100, 500 or 2,500 mg/kg body weight. Isolated peritoneal macrophages were stimulated with mitogen (LPS; 1 mg/mL) for 24 hr. The concentrations of IL-6 were determined by triplicates cultured supernatant cells and values are mean±S.D. (n=4). Statistical analysis was performed using student's two tails t-test with a significant level of *p<0.05, **p<0.01 compared to 0 (Control group). NAOs; Neoagarooligosaccharides.
그림 Fig. 4. TNF-a production by activated peritoneal macrophages of mice orally administrated with neoagarooligosaccharides from marine red algae for 4 weeks. NAOs was orally treated for 4 weeks (twice/week) at the concentration of 100, 500 or 2,500 mg/kg body weight. Isolated peritoneal macrophages were stimulated with mitogen (LPS; 1 mg/mL) for 24 hr. The concentrations of TNF-a were determined by triplicates cultured supernatant cells and values are mean±S.D. (n=4). Statistical analysis was performed using student's two tails t-test with a significant level of *p<0.05, **p<0.01 compared to 0 (Control group). NAOs; Neoagarooligosaccharides.

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