[논문]마카 품종별 조다당 획분의 대식세포 활성

신현영; 김훈; 정은진; 유광원

doi:10.9799/ksfan.2022.35.1.007

마카 품종별 조다당 획분의 대식세포 활성
Macrophage Stimulating Activity of Crude Polysaccharide on Maca (Lepidium meyenii) Varieties 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.35 no.1, 2022년, pp.7 - 15

신현영 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템융합전공) , 김훈 (중앙대학교 생명공학대학) , 정은진 (한국교통대학교 식품영양학전공) , 유광원 (한국교통대학교 식품영양학전공)

Abstract ▼ AI-Helper

Maca roots (Lepidium meyenii) are an important medicinal herb that have long been used by the Andes-indigenous peoples and South Americans. In Korea, recently, it has attracted attention as a health food material because of nutritional values and physiological activities. The purpose of this study was to investigate the industrial applicability of maca (red and golden varieties; R&G) as immunostimulating materials. In the macrophage stimulating assay using RAW 264.7 cells at 125~500 ㎍/mL of non-cytotoxicity doses, G-HW showed the most potent production of TNF-α, IL-6 and nitric oxide compared to red maca or the other extracts. The general component analysis results showed that all extracts comprised more than 90% neutral sugars with small amounts of uronic acid and protein. Meanwhile, component sugar analysis showed the difference in the content of uronic acids of cold- and hot-water extract. Additionally, the further fractionation of G-HW into crude polysaccharide (G-CP) greatly enhanced the macrophage stimulating activity, and G-CP contained macromolecules over 144 kDa, comprised mainly of glucose and galacturonic acid (51.0 and 34.9%). In conclusion, crude polysaccharide from maca showed industrial applicability as immunostimulating material, and especially golden maca showed higher macrophage stimulating activity than red maca.

주제어

표/그림 (6)

표 Table 1. Analytical condition of HPLC applied for determining the sugar composition and molecular weight of maca
표 Table 2. Yields and chemical properties of various water extracts from red and golden maca
표 Table 3. Chemical property of crude polysaccharide from hot-water extract of golden maca
그림 Fig. 1. Macrophage stimulating activity of water extract from red (R) or golden (G) maca. Macrophage stimulating activity evaluates RAW 264.7 cell line as macrophage stimulating related factors produced after cultivating 24 hr with each extract. (A): RAW 264.7 cell toxicity, (B)~(D): production of TNF-α, IL-6 and nitric oxide (NO). Results are expressed as mean±S.D. of three independent test in triplicate. Different asterisks on the column indicate significant difference between negative control (NC) and each group by Student’s t-test. *p<0.05, **p<0.01, ***p<0.001. NC; only medium was used as negative control, LPS (lipopolysaccharide, 1 μg/mL); positive control, CW or HW; cold-water extract or hot-water extract.
그림 Fig. 2. Macrophage stimulating activity of crude polysaccharide from hot-water extract of golden (G) maca. Macrophage stimulating activity evaluates RAW 264.7 cell line as macrophage stimulating related factors produced after cultivating 24 hr with crude polysaccharide (G-CP). (A): RAW 264.7 cell-toxicity, (B)~(D): production of TNF-α, IL-6 and nitric oxide (NO). Results are expressed as mean±S.D. of three independent test in triplicate. Different asterisks on the column indicate significant difference between negative control (NC) and each group by Student's t-test. *p<0.05, **p<0.01, ***p<0.001. NC; only medium was used as negative control, LPS (lipopolysaccharide, 1 μg/mL); positive control, HW; hot-water extract, G-CP; crude polysaccharide from HW.
$Fig. 3. HPLC chromatogram of molecular weight distribution of (A) G-HW, and (B) G-CP fractionated from golden (G) maca. Hot-water extract (HW) and crude polysaccharide (CP) were applied to the HPLC-ELSD system at dose of 10 mg/mL.$ 그림 Fig. 3. HPLC chromatogram of molecular weight distribution of (A) G-HW, and (B) G-CP fractionated from golden (G) maca. Hot-water extract (HW) and crude polysaccharide (CP) were applied to the HPLC-ELSD system at dose of 10 mg/mL.

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