[논문]LPS로 유도된 RAW 264.7 대식세포에서 청대의 항염증효과

장수주; 강순아

doi:10.9799/ksfan.2022.35.2.116

LPS로 유도된 RAW 264.7 대식세포에서 청대의 항염증효과
Anti-Inflammatory Effect of Chung-Dae in LPS-Treated RAW 264.7 Cells 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.35 no.2, 2022년, pp.116 - 126

장수주 (호서대학교 보건산업연구소) , 강순아 (호서대학교 벤처대학원 융합공학과)

Abstract ▼ AI-Helper

The purpose of this study was to analyze the anti-inflammatory effect of Chung-Dae Indigo Pulverata Levis, indigo naturalis) produced during indigo dyeing. As a result of in vitro cytotoxicity experiments using RAW 264.7 cell, Chung-Dae extract did not inhibit cell proliferation in Raw 264.7 cells in the range of 1~32 ㎍/mL. NO production was significantly reduced when Chung-Dae extracts were treated at concentrations of 2, 8, and 32 ㎍/mL (p<0.05). The pro-inflammatory cytokines TNF-α, IL-6, IL-1β and IFN-γ significantly decreased when the Chung-Dae extract was treated at concentrations of 2, 8, and 32 ㎍/mL compared to the LPS group, and similarly, the TNFα and IL-6 mRNA levels also decreased. Additionally, the mRNA level of COX-2 was also suppressed. At the protein expression level, the expression of TNF-α, IL-6, iNOS and COX-2 were observed with LPS and Chung-Dae extract significantly decreased compared to the group treated with only LPS (p<0.05). From the above results, it shows that Chung-Dae extract, a plant-derived compound, inhibits the inflammatory response induced by LPS in RAW 264.7 cells. and in particular, regulates the inflammatory response by inhibiting the expression of pro-inflammatory cytokines and inflammation-related enzymes.

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그림 Fig. 1. Effect of Chung-Dae on RAW 264.7 cell viability (a) and Chung-Dae on LPS-induced RAW 264.7 cell (b). LPS: treated with LPS (2 μg/mL), Chung-Dae: treated with various concentrations (2, 8, and 32 μg/mL) of Chung-Dae with LPS (2 μg/mL). ^a-dMeans with the different letters on the bars are significantly different (p<0.05) by Duncan's multiple range tests.
그림 Fig. 2. Effect of Chung-Dae on NO production in LPS-induced RAW 264.7 cell. LPS: treated with LPS (2 μ g/mL), Chung-Dae: treated with various concentrations (2, 8, and 32 μg/mL) of Chung-Dae with LPS (2 μg/mL). ^a-eMeans with the different letters on the bars are significantly different (p<0.05) by Duncan's multiple range tests.
그림 Fig. 3. Effect of Chung-Dae on pro-inflammatory cytokines TNF-α and IFN-γ expression level in LPS-induced RAW 264.7 cell. LPS: treated with LPS (2 μg/mL), Chung-Dae: treated with various concentrations (2, 8, and 32 μg/mL) of Chung-Dae with LPS (2 μg/mL). ^a-dMeans with the different letters on the bars are significantly different (p<0.05) by Duncan's multiple range tests.
그림 Fig. 4. Effect of Chung-Dae on pro-inflammatory cytokines IL-6 and IL-1β expression level in LPS-induced RAW 264.7 cell. LPS: treated with LPS (2 μg/mL), Chung-Dae: treated with various concentrations (2, 8, and 32 μg/mL) of Chung-Dae with LPS (2 μg/mL). ^a-eMeans with the different letters on the bars are significantly different (p<0.05) by Duncan's multiple range tests.
그림 Fig. 5. Effect of Chung-Dae on mRNA expression of TNF-α, IL-6 and COX-2 in LPS-induced RAW 264.7 cell. LPS: treated with LPS (2 μg/mL), Chung-Dae: treated with various concentrations (2, 8, and 32 μg/mL) of Chung-Dae with LPS (2 μg/mL). ^a-dMeans with the different letters on the bars are significantly different (p<0.05) by Duncan's multiple range tests.
그림 Fig. 6. Effect of Chung-Dae on protein expression of TNF-α and IL-6 in LPS-induced RAW 264.7 cell. LPS: treated with LPS (2 μg/mL), Chung-Dae: treated with various concentrations (2, 8, and 32 μg/mL) of Chung-Dae with LPS (2 μg/mL). ^a-dMeans with the different letters on the bars are significantly different (p<0.05) by Duncan's multiple range tests.
그림 Fig. 7. Effect of Chung-Dae on protein expression of iNOS and COX-2 in LPS-induced RAW 264.7 cell. LPS: treated with LPS (2 μg/mL), Chung-Dae: treated with various concentrations (2, 8, and 32 μg/mL) of Chung-Dae with LPS (2 μ g/mL). ^a-cMeans with the different letters on the bars are significantly different (p<0.05) by Duncan’s multiple range tests.

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