[논문]농업지역 지하수의 수리지화학 및 미생물 군집 구조 분석

김동훈; 오용화; 이봉주; 이정윤

doi:10.7857/jsge.2022.27.2.061

농업지역 지하수의 수리지화학 및 미생물 군집 구조 분석
Hydrogeochemistry and Microbial Community Structure of Groundwater in an Agricultural Area 원문보기

지하수토양환경 = Journal of soil and groundwater environment, v.27 no.2, 2022년, pp.61 - 75

김동훈 (한국지질자원연구원 기후변화대응연구본부 지하수환경연구센터) , 오용화 (한국해양대학교 해양과학기술전문대학원 해양과학기술융합학과) , 이봉주 (한국지질자원연구원 기후변화대응연구본부 지하수환경연구센터) , 이정윤 (한국지질자원연구원 기후변화대응연구본부 지하수환경연구센터)

Abstract ▼ AI-Helper

This study evaluated the potential threat of agricultural and human activities to groundwater in the Noseong stream watershed, a typical agricultural area, through hydrogeochemical characteristics and microbial community analyses. The groundwater in the study area was Ca-SO4 and Ca-HCO3 types alluvial aquifer mainly used for agricultural and living purposes, and contained high levels of NO3- and Cl- ions generated from anthropogenic sources such as fertilizer, livestock wastewater, and domestic sewage. Proteobacteria was most abundant in all samples with an average of 46.1% while Actinobacteria, Bacteroidetes, and Cyanobacteria were dominant on an occasional basis. The prevalence of aerobic bacteria such as the genus Mycobacterium, Flavobacterium, and Sphingomonas suggests that groundwater was well connected with the surface layer. The potential pathogen Mycobacterium was detected in most samples, and other pathogenic bacteria were also widely distributed, indicating the vulnerability to contamination. Therefore, an integrated management system is required to secure the sustainable use of groundwater in agricultural areas with high groundwater dependence.

주제어

표/그림 (10)

그림 Fig. 1. Sampling locations of groundwater wells in the study area (Noseong stream watershed).
그림 Fig. 2. Box and whisker plots showing statistical analysis results for the concentrations of (a) major cations (Ca, Mg, Na, K, and SiO₂ (aq)) and (b) major anions (HCO₃, Cl, NO₃, and SO₄). The edges of the boxes are the 25%and 75% quartiles and the bars indicate the 10% and 90% data spread. Red line presents the mean value and outliers are indicated by solid circles.
표 Table 1. Field parameters measured in this study
그림 Fig. 3. Piper diagram for the groundwater samples collected from study area.
그림 Fig. 4. Gibbs diagrams showing the ratio of TDS and Na⁺/(Na⁺ + Ca²⁺) or Cl^-/(Cl^- + HCO₃^-) and indicating mechanisms controlling the groundwater chemistry in the study area.
표 Table 2. Pearson's correlation matrix of groundwater hydrogeochemical parameters
그림 Fig. 5. Microbial taxonomic compositions of groundwater samples at plylum (a) and class (b) level.
표 Table 3. Alpha diversity indices at the 97% OUT level of groundwater samples
그림 Fig. 6. Heatmap illustrating abundance of the OTUs at the genus level. Genera are present for top twenty OTUs those whose highest sequence abundance across the samples. Dendrograms on the vertical and horizontal axes are based on the Bray_Curtis dissimilarity matrix.
그림 Fig. 7. Relative abundance of potential pathogenic bacteria in groundwater.

AI 본문요약
AI-Helper

문제 정의

따라서 인간 활동에 의한 지하수 오염 특성을 파악하고 오염 가능성을 예측하여 사전에 오염을 예방할 수 있는 통합적인 관리 방안이 반드시 필요하다. 이러한 관점에서 본 연구는 농업 활동이 활발하고 음용수, 생활 용수 및 농업용수로 이용되고 있는 노성천 유역 지하수의 잠재적 위험성을 평가하기 위해 수질 및 미생물학적 특성 분석을 수행하였다.

참고문헌 (74)

Allende, A. and Monaghan, J., 2015, Irrigation water quality for leafy crops: A perspective of risks and potential solutions, Int. J. Environ. Res. Public Health, 12(7), 7457-7477.

상세보기
Balkwill, D.L., Fredrickson, J.K., and Romine, M.F., 2006, Sphingomonas and related genera. In: Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K.-H., Stackebrandt, E. (Eds.), The Prokaryotes: Volume 7: Proteobacteria: Delta, Epsilon Subclass. Springer New York, New York, NY, p. 605-629.
Bratanis, E., Andersson, T., Lood, R., and Bukowska-Faniband, E., 2020, Biotechnological potential of Bdellovibrio and like organisms and their secreted enzymes, Front. Microbiol., 11(662).
Caporaso, J.G., Kuczynski, J., Stombaugh, J., Bittinger, K., Bushman, F.D., Costello, E.K., Fierer, N., Pena, A.G., Goodrich, J.K., Gordon, J.I., Huttley, G.A., Kelley, S.T., Knights, D., Koenig, J.E., Ley, R.E., Lozupone, C.A., McDonald, D., Muegge, B.D., Pirrung, M., Reeder, J., Sevinsky, J.R., Turnbaugh, P.J., Walters, W.A., Widmann, J., Yatsunenko, T., Zaneveld, J., and Knight, R., 2010, Qiime allows analysis of high-throughput community sequencing data, Nat. Methods, 7(5), 335-336.

상세보기
Cho, B.-W., Yun, U., Lee, B.-D., and Ko, K.-S., 2012, Hydrogeological characteristics of the wangjeon-ri PCWC area, Nonsan-city, with an emphasis on water level variations, The J. Eng. Geol., 22(2), 195-205.
De Vet, W., Dinkla, I., Abbas, B., Rietveld, L., and Van Loosdrecht, M., 2012, Gallionella spp. in trickling filtration of subsurface aerated and natural groundwater, Biotechnol. Bioeng., 109(4), 904-912.

상세보기
De Vet, W., Dinkla, I., Rietveld, L., and Van Loosdrecht, M., 2011, Biological iron cxidation by Gallionella spp. in drinking water production under fully aerated conditions, Water Res., 45(17), 5389-5398.
de Voogd, N.J., Cleary, D.F.R., Polonia, A.R.M., and Gomes, N.C.M., 2015, Bacterial community composition and predicted functional ecology of sponges, sediment and seawater from the thousand islands reef complex, West Java, Indonesia, FEMS Microbiol. Ecol., 91(4).

상세보기
Delafont, V., Samba-Louaka, A., Cambau, E., Bouchon, D., Moulin, L., and Hechard, Y., 2017, Mycobacterium llatzerense, a waterborne Mycobacterium, that resists phagocytosis by Acanthamoeba castellanii, Sci. Rep., 7(1), 46270.
Edgar, R.C., 2010, Search and clustering orders of magnitude faster than blast, Bioinform., 26(19), 2460-2461.
Forbes, B.A., Hall, G.S., Miller, M.B., Novak, S.M., Rowlinson, M.-C., Salfinger, M., Somoskovi, A., Warshauer, D.M., and Wilson, M.L., 2018, Practical guidance for clinical microbiology laboratories: Mycobacteria, Clin. Microbiol. Rev., 31(2), e00038-00017.
Ghilamicael, A.M., Boga, H.I., Anami, S.E., Mehari, T., and Budambula, N.L.M., 2018, Potential human pathogenic bacteria in five hot springs in eritrea revealed by next generation sequencing, PLoS One, 13(3), e0194554.

상세보기
Gibbs, R.J., 1970, Mechanisms controlling world water chemistry, Science, 170(3962), 1088-1090.

상세보기
Griebler, C. and Lueders, T., 2009, Microbial biodiversity in groundwater ecosystems, Freshw. Biol., 54(4), 649-677.

상세보기
Hallbeck, L. and Pedersen, K., 2014, The family Gallionellaceae. In: Rosenberg, E., DeLong, E.F., Lory, S., Stackebrandt, E., Thompson, F. (Eds.), The Prokaryotes: Alphaproteobacteria and Betaproteobacteria. Springer Berlin Heidelberg, Berlin, Heidelberg, p. 853-858.
Hassan, Z., Sultana, M., van Breukelen, B.M., Khan, S.I., and Roling, W.F., 2015, Diverse arsenic- and iron-cycling microbial communities in arsenic-contaminated aquifers used for drinking water in Bangladesh, FEMS Microbiol. Ecol., 91(4).

상세보기
Hlavinek, P., Bonacci, O., Marsalek, J., and Mahrikova, I., 2008, Dangerous pollutants (Xenobiotics) in urban water cycle. Nato Science for Peace and Security Series. Springer, Dordrecht.
Hounslow, A., 2018, Water quality data: Analysis and interpretation, p. 1-398.
Huntley, S., Hamann, N., Wegener-Feldbrugge, S., TreunerLange, A., Kube, M., Reinhardt, R., Klages, S., Muller, R., Ronning, C.M., Nierman, W.C., and Sogaard-Andersen, L., 2010, Comparative genomic analysis of fruiting body rormation in Myxococcales, Mol. Biol. Evol., 28(2), 1083-1097.
Ibekwe, A.M., Leddy, M., and Murinda, S.E., 2013, Potential human pathogenic bacteria in a mixed urban watershed as revealed by pyrosequencing, PLoS One, 8(11), e79490.

상세보기
Janniche, G.S., Spliid, H., and Albrechtsen, H.J., 2012, Microbial community-level physiological profiles (CLPP and herbicide mineralization potential in groundwater affected by agricultural land use, J. Contam. Hydrol., 140-141, 45-55.
Jeong, C.H., Yang, J.H., Lee, Y.J., Lee, Y.C., Choi, H.Y., Kim, M.S., Kim, H.K., Kim, T.S., and Jo, B.U., 2015, Occurrences of uranium and radon-222 from groundwaters in various geological environment in the Hoengseong area., The J. Eng. Geol., 25(4), 557-576.
Kanamori, H., Weber, D.J., and Rutala, W.A., 2016, Healthcare outbreaks associated with a water reservoir and infection prevention strategies, Clin. Infect. Dis., 62(11), 1423-1435.

상세보기
Kasalicky, V., Jezbera, J., Hahn, M.W., and Simek, K., 2013, The diversity of the Limnohabitans genus, an important group of freshwater bacterioplankton, by characterization of 35 isolated strains, PLoS One, 8(3), e58209.

상세보기
Katsoyiannis, I.A. and Zouboulis, A.I., 2006, Use of iron-and manganese-oxidizing bacteria for the combined removal of iron, manganese and arsenic from contaminated groundwater, Water Qual. Rese. J., 41(2), 117-129.
Kersters, K., De Vos, P., Gillis, M., Swings, J., Vandamme, P., and Stackebrandt, E., 2006, Introduction to the proteobacteria. In: Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K.-H., Stackebrandt, E. (Eds.), The Prokaryotes: Volume 5: Proteobacteria: Alpha and Beta Subclasses. Springer New York, New York, NY, p. 3-37.
KIGAM, 2019, Integrated technology development for securing groundwater/geothermal resources and conserving ecosystem according to climate change.
Kim, D.-H., Moon, S.-H., Ko, K.-S., and Kim, S., 2020, Microbial community structures related to arsenic concentrations in groundwater occurring in Haman area, South Korea, Econ. Environ. Geol., 53(6), 655-666.
Kim, H., Kaown, D., Mayer, B., Lee, J.-Y., Hyun, Y., and Lee, K.-K., 2015, Identifying the sources of nitrate contamination of groundwater in an agricultural area (Haean basin, Korea) using isotope and microbial community analyses, Sci. Total Environ., 533, 566-575.

상세보기
Kim, K.-H., Yun, S.-T., Chae, G.-T., Choi, B.-Y., Kim, S.-O., Kim, K., Kim, H.-S., and Lee, C.-W., 2002, Nitrate contamination of alluvial groundwaters in the Keum river watershed area: Source and behaviors of nitrate, and suggestion to secure water supply, The J. Eng. Geol., 12(4), 471-484.
Ko, K.-S., Ahn, J.-S., Suk, H.-J., Lee, J.-S., and Kim, H.-S., 2008, Hydrogeochemistry and statistical analysis of water quality for small potable water supply system in Nonsan area, J. Soil and Groundw. Environ., 13(6), 72-84.
Korbel, K., Chariton, A., Stephenson, S., Greenfield, P., and Hose, G.C., 2017, Wells provide a distorted view of life in the aquifer: Implications for sampling, monitoring and assessment of groundwater ecosystems, Sci. Rep., 7(1), 40702.
Kwon, H.-I., Koh, D.-C., Jung, B., and Ha, K., 2017, Quantification of seasonally variable water flux between aquifer and stream in the riparian zones with water curtain cultivation activities using numerical simulation., J. Geol. Soc. Korea, 53, 277-290.
Kwon, H.-I., Koh, D.-C., Jung, Y.-Y., Kim, D.-H., and Ha, K., 2020, Evaluating the impacts of intense seasonal groundwater pumping on stream-aquifer interactions in agricultural riparian zones using a multi-parameter approach, J. Hydrol., 584, 124683.

상세보기
Lacroix, B. and Citovsky, V., 2013. Agrobacterium. In: Maloy, S., Hughes, K. (Eds.), Brenner's encyclopedia of genetics (Second Edition). Academic Press, San Diego, p. 52-54.
Lall, U., Josset, L., and Russo, T., 2020, A snapshot of the world's groundwater challenges, Annu. Rev. Environ. Resour., 45, 171-194.
Lee, G.-M., Park, S., Kim, K.-I., Jeon, S.-H., Song, D., Kim, D.-h., Kim, T.-S., Yun, S.-T., Chung, H.M., and Kim, H.-K., 2017, Evaluation for impacts of nitrogen source to groundwater quality in livestock farming area, Korean J. Soil Sci. Fert., 50(5), 345-356.

원문보기 상세보기
Lee, J.-C., Kim, S.-G., and Whang, K.-S., 2015, Sphingobium subterraneum sp. Nov., isolated from ground water, Int. J. Syst. Evol. Microbiol., 65(Pt_2), 393-398.

상세보기
Lee, J.-H., Lee, B.-J., and Unno, T., 2018, Bacterial communities in ground-and surface water mixing zone induced by seasonal heavy extraction of groundwater, Geomicrobiol. J., 35(9), 768-774.

상세보기
Li, W., Fu, L., Niu, B., Wu, S., and Wooley, J., 2012, Ultrafast clustering algorithms for metagenomic sequence analysis, Brief. Bioinform., 13(6), 656-668.
Moon, J.-T., Kim, K.-J., Kim, S.-H., Jeong, C.-S., and Hwang, G.-S., 2008, Geochemical investigation on arsenic contamination in the alluvial ground-water of Mankyeong river watershed, Econ. Environ. Geol., 41(6), 673-683.
Mueller, D.K. and Helsel, D., 1996, Nutrients in the nation's waters--too much of a good thing?
Nelson, W.C. and Stegen, J.C., 2015, The teduced genomes of Parcubacteria (OD1) contain signatures of a symbiotic lifestyle, Front. Microbiol., 6, 713-713.
Oh, S. and Choi, D., 2019, Microbial community enhances biodegradation of bisphenol a through selection of Sphingomonadaceae, Microb. Ecol., 77(3), 631-639.

상세보기
Oh, Y.H., Koh, D.-C., Kwon, H.-I., Jung, Y.-Y., Lee, K.Y., Yoon, Y.-Y., Kim, D.-H., Moon, H.S., and Ha, K., 2021, Identifying and quantifying groundwater inflow to a stream using 220Rn and 222Rn as natural tracers, J. Hydrol. Reg. Stud., 33, 100773.
Oh, Y.H., Kim, D.-H., Lee, S.-H., Moon, H.S., and Cho, S.Y., 2020, Determining characteristics of groundwater inflow to the stream in an urban area using hydrogeochemical tracers (222Rn and major dissolved ions) and microbial community analysis, J. Soil Groundw. Environ., 25(2), 16-23.
Orata, F.D., Meier-Kolthoff, J.P., Sauvageau, D., and Stein, L.Y., 2018, Phylogenomic analysis of the gammaproteobacterial methanotrophs (rrder Methylococcales) calls for the reclassification of members at the genus and species levels, Front. Microbiol., 9(3162).
Oren, A., 2014, The family Rhodocyclaceae. In: Rosenberg, E., DeLong, E.F., Lory, S., Stackebrandt, E., Thompson, F. (Eds.), The Prokaryotes: Alphaproteobacteria and Betaproteobacteria. Springer Berlin Heidelberg, Berlin, Heidelberg, p. 975-998.
Pachepsky, Y., Shelton, D.R., McLain, J.E.T., Patel, J., and Mandrell, R.E., 2011, Irrigation waters as a source of pathogenic microorganisms in produce: a review, Adv. Agron., 113, 75-141.
Pagadala, S., Marine, S.C., Micallef, S.A., Wang, F., Pahl, D.M., Melendez, M.V., Kline, W.L., Oni, R.A., Walsh, C.S., Everts, K.L., and Buchanan, R.L., 2015, Assessment of region, farming system, irrigation source and sampling time as food safety risk factors for tomatoes, Int. J. Food. Microbiol., 196, 98-108.

상세보기
Pedersen, K., 2011, Gallionella. In: Reitner, J., Thiel, V. (Eds.), Encyclopedia of Geobiology. Springer Netherlands, Dordrecht, p. 411-412.
Percival, S.L. and Williams, D.W., 2014, Chapter Nine - Mycobacterium. In: Percival, S.L., Yates, M.V., Williams, D.W., Chalmers, R.M., Gray, N.F. (Eds.), Microbiology of Waterborne Diseases (Second Edition). Academic Press, London, p. 177-207.
Piper, A.M., 1944, A Graphic procedure in the geochemical interpretation of water-analyses, Eos, Transactions American Geophysical Union, 25(6), 914-928.
Ramirez-Castillo, F.Y., Loera-Muro, A., Jacques, M., Garneau, P., Avelar-Gonzalez, F.J., Harel, J., and Guerrero-Barrera, A.L., 2015, Waterborne pathogens: detection methods and challenges, Pathogens, 4(2), 307-334.

상세보기
Saether, O.M. and De Caritat, P., 1996, Geochemical processes, weathering and groundwater recharge in catchments. CRC Press.
Sang, S., Zhang, X., Dai, H., Hu, B.X., Ou, H., and Sun, L., 2018, Diversity and predictive metabolic pathways of the prokaryotic microbial community along a groundwater salinity gradient of the Pearl river delta, China, Sci. Rep., 8(1), 17317.
Schulze-Robbecke, R., 1993, Mycobacteria in the environment, Immun. Infekt., 21(5), 126-131.
Sherwood, W.C., 1989, Chloride loading in the south fork of the Shenandoah river, Virginia, U.S.A, Environ. Geol., 14, 99-106.
Slover, C.M. and Danziger, L., 2008, Lactobacillus: a review, Clin. Microbiol. Newsl., 30(4), 23-27.

상세보기
Spanevello, M.D. and Patel, B.K.C., 2004, The phylogenetic diversity of Thermus and Meiothermus from microbial mats of an Australian subsurface aquifer runoff channel, FEMS Microbiol. Ecol., 50(1), 63-73.

상세보기
Stites, W. and Kraft, G.J., 2001, Nitrate and chloride loading to groundwater from an irrigated north-central U.S. sand-plain vegetable field, J. Environ. Qual., 30(4), 1176-1184.

상세보기
Stoecker, K., Bendinger, B., Schoning, B., Nielsen, P.H., Nielsen, J.L., Baranyi, C., Toenshoff, E.R., Daims, H., and Wagner, M., 2006, Cohn's Crenothrix is a filamentous methane oxidizer with an unusual methane monooxygenase, Proc. Natl. Acad. Sci., 103(7), 2363-2367.
Thomas, F., Hehemann, J.-H., Rebuffet, E., Czjzek, M., and Michel, G., 2011, Environmental and gut Bacteroidetes: the food connection, Front. Microbiol., 2, 93-93.

상세보기
UN Water, 2018, Progress on level of water stress: global baseline for SDG indicator 6.4. 2, UN Water, Geneva (2018).
Uyttendaele, M., Jaykus, L.-A., Amoah, P., Chiodini, A., Cunliffe, D., Jacxsens, L., Holvoet, K., Korsten, L., Lau, M., McClure, P., Medema, G., Sampers, I., and Rao Jasti, P., 2015, Microbial hazards in irrigation water: standards, norms, and testing to manage use of water in fresh produce primary production, Comp. Rev. Food Sci. Food Saf., 14(4), 336-356.
Van Der Linden, I., Cottyn, B., Uyttendaele, M., Berkvens, N., Vlaemynck, G., Heyndrickx, M., and Maes, M., 2014, Enteric pathogen survival varies substantially in irrigation water from Belgian lettuce producers, Int. J. Environ. Re.s Public Health, 11(10), 10105-10124.

상세보기
Wang, W., Wang, H., Feng, Y., Wang, L., Xiao, X., Xi, Y., Luo, X., Sun, R., Ye, X., Huang, Y., Zhang, Z., and Cui, Z., 2016, Consistent responses of the microbial community structure to organic farming along the middle and lower reaches of the Yangtze river, Sci. Rep., 6(1), 35046.
Waskiewicz, A. and Irzykowska, L., 2014, Flavobacterium spp. - characteristics, occurrence, and toxicity. In: Batt, C.A., Tortorello, M.L. (Eds.), Encyclopedia of Food Microbiology (Second Edition). Academic Press, Oxford, p. 938-942.
Wolfe, R., 1960, Observations and studies of Crenothrix polyspora, J. Am. Water Work. Assoc., 52, 915-918.
Yang, J.H., Kim, H.-K., Kim, M., Lee, M.K., Shin, I.K., Park, S.H., Kim, H.S., Ju, B.K., Kim, D.S., and Kim, T.S., 2015, Evaluation of groundwater quality deterioration using the hydrogeochemical characteristics of shallow portable groundwater in an agricultural area, The J. Eng. Geol., 25(4), 533-545.
Yoon, J., Park, S., Choi, H., Kim, D.H., Kim, M., Yun, S.-T., Kim, Y., and Kim, H.-K., 2020, Analysis of groundwater quality and contamination factors in livestock region, South Korea, J. Soil Groundw. Environ., 25(4), 98-105.
Yoon, K.-S., Tsukada, N., Sakai, Y., Ishii, M., Igarashi, Y., and Nishihara, H., 2008, Isolation and characterization of a new facultatively autotrophic hydrogen-xxidizing betaproteobacterium, Hydrogenophaga sp. AH-24, FEMS Microbiol. Lett., 278(1), 94-100.
Zeng, X., Hosono, T., Matsunaga, M., Ohta, H., Niidome, T., Shimada, J., and Morimura, S., 2017, Spatial distribution of microbial communities in the alluvial aquifer along the Oyodo river, Miyakonojo basin, Japan, J. Water Environ. Technol., 15(4), 152-162.
Zhang, Q., Wu, J., Yang, F., Lei, Y., Zhang, Q., and Cheng, X., 2016, Alterations in soil microbial community composition and biomass following agricultural land use change, Sci. Rep., 6(1), 36587.

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