[논문]Resistance Induction by Salicylic Acid Formulation in Cassava Plant against Fusarium solani

Saengchan, Chanon; Phansak, Piyaporn; Thumanu, Kanjana; Siriwong, Supatcharee; Le Thanh, Toan; Sangpueak, Rungthip; Thepbandit, Wannaporn; Papathoti, Narendra Kumar; Buensanteai, Natthiya

doi:10.5423/ppj.oa.02.2022.0019

Resistance Induction by Salicylic Acid Formulation in Cassava Plant against Fusarium solani 원문보기

The plant pathology journal, v.38 no.3, 2022년, pp.212 - 219

Saengchan, Chanon (School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology) , Phansak, Piyaporn (Division of Biology, Faculty of Science, Nakhon Phanom University) , Thumanu, Kanjana (Synchrotron Light Research Institute) , Siriwong, Supatcharee (Synchrotron Light Research Institute) , Le Thanh, Toan (Department of Plant Protection, College of Agriculture, Can Tho University) , Sangpueak, Rungthip (School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology) , Thepbandit, Wannaporn (School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology) , Papathoti, Narendra Kumar (R&D Division, Sri Yuva Biotech Pvt Ltd) , Buensanteai, Natthiya (School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology)

Abstract ▼ AI-Helper

Fusarium root rot caused by the soil-borne fungus Fusarium solani is one of the most important fungal diseases of cassava in Thailand, resulting in high yield losses of more than 80%. This study aimed to investigate if the exogenous application of salicylic acid formulations (Zacha) can induce resistance in cassava against Fusarium root rot and observe the biochemical changes in induced cassava leaf tissues through synchrotron radiation based on Fourier-transform infrared (SR-FTIR) microspectroscopy. We demonstrated that the application of Zacha11 prototype formulations could induce resistance against Fusarium root rot in cassava. The in vitro experimental results showed that Zacha11 prototype formulations inhibited the growth of F. solani at approximately 34.83%. Furthermore, a significant reduction in the disease severity of Fusarium root rot disease at 60 days after challenge inoculation was observed in cassava plants treated with Zacha11 at a concentration of 500 ppm (9.0%). Population densities of F. solani were determined at 7 days after inoculation. Treatment of the Zacha11 at a concentration of 500 ppm resulted in reduced populations compared with the distilled water control and differences among treatment means at each assay date. Moreover, the SR-FTIR spectral changes of Zacha11-treated epidermal tissues of leaves had higher integral areas of lipids, lignins, and pectins (1,770-1,700/cm), amide I (1,700-1,600/cm), amide II (1,600-1,500/cm), hemicellulose, lignin (1,300-1,200/cm), and cellulose (1,155/cm). Therefore, alteration in defensive carbohydrates, lipids, and proteins contributed to generate barriers against Fusarium invasion in cassava roots, leading to lower the root rot disease severity.

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표/그림 (6)

표 Table 1. Antifungal activity of salicylic acid (Zacha formulations) against Fusarium solani using a dual culture test
그림 Fig. 1. The inhibition of the mycelial growth of Fusarium in Petri dishes with the different treatments at 7 days after putting fungal slices. Sterile Whatman filter paper discs of 6 mm diameter impregnated 500 ppm Zacha11 formulations (A), Mancozeb at a concentration of 2,000 μg/ml was used as a positive control (B) and negative control (C).
그림 Fig. 2. Population densities of Fusarium solani infested soil at 7 days after inoculation under greenhouse conditions and with treated Zacha 11, Mancozeb (80% Mancozeb as the active ingredient), and distilled water control.
표 Table 2. Efficacy of salicylic acid (Zacha formulations) on the severity of Fusarium root rot disease caused by Fusarium solani under greenhouse conditions at 7 days after inoculation
그림 Fig. 3. Principal component analysis (PCA) and average 2nd derivative spectrum of the epidermis of leaf tissues treated with 500 ppm salicylic acid formulation (Zacha11) and compared with Mancozeb, water, and after challenge inoculation with Fusarium solani at 7 DAI. (A) 2D scatter plot of score from PCA analysis. (B) The second derivative in epidermis tissues of cassava leaf.
표 Table 3. Band assignments of FTIR vibration peak (/cm) of plant tissues

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표제어: PCR

동의어: Packet Collision Rate

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