[논문]인간 제대정맥 내피세포에서 산수유와 산수유청혈플러스의 항염증효과

김정희; 유호룡; 설인찬; 김윤식

doi:10.13048/jkm.22034

인간 제대정맥 내피세포에서 산수유와 산수유청혈플러스의 항염증효과
Anti-inflammatory Effect of Cornus Officinalis fruit extract and Cornus Officinalis Fruit Cheonghyeol Plus in Human Umbilical Vein Endothelial Cell 원문보기

Journal of Korean Medicine = 대한한의학회지, v.43 no.3, 2022년, pp.106 - 121

김정희 (대전대학교 한의과대학 심계내과학교실) , 유호룡 (대전대학교 한의과대학 심계내과학교실) , 설인찬 (대전대학교 한의과대학 심계내과학교실) , 김윤식 (대전대학교 한의과대학 심계내과학교실)

Abstract ▼ AI-Helper

Objectives: The purpose of this study was to investigate the anti-inflammatory effect of Cornus Officinalis fruit extract(CE) and Cornus Officinalis Fruit Cheonghyeol Plus(CCP) in Human Umbilical Vein Endothelial Cell. Methods: We measured cell viability of CE, CCP and treated HUVEC with TNF-α. We measured the mRNA expression levels of KLF2, eNOS, MCP-1, ICAM-1, VCAM-1, the protein expression levels of KLF2, eNOS, MCP-1, ICAM-1, VCAM-1, and the protein phosphorylation level of ERK, JNK, p38 and the biomarker expression levels of MCP-1, ICAM-1, VCAM-1. Results: 1.CE incresed the mRNA, protein expression levels of KLF2, eNOS at concentrations of 100㎍/㎖ compared to the control group. CE decresed the mRNA, protein and biomarker expression levels of MCP-1,ICAM-1,VCAM-1 at concentrations of 100㎍/㎖ compared to the control group. CE decresed the protein phosphorylation level of p38 at concentrations of 100㎍/㎖ compared to the control group. 2. CCP incresed the mRNA, protein expression levels of KLF2, eNOS at concentrations of 100㎍/㎖ or more compared to the control group. CCP decresed the mRNA, protein and biomarker expression levels of MCP-1, ICAM-1, VCAM-1 at concentrations of 100㎍/㎖ or more compared to the control group. CCP decresed the protein phosphorylation level of ERK at concentrations of 100㎍/㎖ or more, p38 at concentrations of 200㎍/㎖ or more, and JNK at concentrations of 400㎍/㎖ compared to the control group. Conclusions: These results present that CE and CCP has anti-inflammatory effect in HUVEC. So, it could help treat or prevent inflammation in vein caused by dyslipidemia and contribute prevention of cardiovascular and cerebrovascular cerebrovascular diseases.

주제어

표/그림 (6)

표 Table 1. Real-Time PCR Primer Sequences
그림 Fig. 1. Cell viability of CE or CCP in HUVEC. Cell viability were calculated as percentage relative to the control and the result were given by the mean±standard error of mean from three independent experiments(Significance of results, *; p<0.05 compared to control).
그림 Fig. 2. Effect of CE or CCP on KLF2(A), eNOS(B), MCP-1(C), ICAM-1(D), VCAM-1(E) mRNA expression level in HUVEC. HUVEC were treated CE(100 ug/ml or CCP(100, 200, and 400 ug/ml), with TNF-α 10 ng/ml for 12hour. The mRNA expression level were measured using a real-time PCR(qPCR). The result were given by the mean±standard error of mean from three independent experiments(Significance of results, *; p<0.05, **; p<0.01, ***; p<0.001 compared to control, ⁺⁺⁺; p<0.001 compared to normal).
그림 Fig. 3. Effect of CE or CCP on KLF2(A), eNOS(B), MCP-1(C), ICAM-1(D), VCAM-1(E) protein expression level in HUVEC. HUVEC were treated CE(100ug/ml or CCP(100, 200, and 400ug/ml), with TNF-α 10 ng/12 hour. The protein expression level were measured by a western blot. The result were presented by the mean±standard error of mean from three independent experiments(Significance of results, *; p<0.05, **; p<0.01, ***; p<0.001 compared to control, ⁺⁺⁺; p<0.001 compared to normal).
그림 Fig. 4. Effect of CE or CCP on ERK(A), JNK(B), p38(C) protein phosphorylation level in HUVEC. HUVEC were treated CE(100ug/ml or CCP(100, 200, and 400ug/ml), with TNF-α 10 ng/ 12 hour. The protein phosphorylation level were measured by a western blot. The result were presented by the mean±standard error of mean from three independent experiments(Significance of results, *; p<0.05, **; p<0.01, ***; p<0.001 compared to control, ⁺⁺⁺; p<0.001 compared to normal).
그림 Fig. 5. Effect of CE or CCP on MCP-1(A), ICAM-1(B), VCAM-1(C) level in HUVEC. HUVEC were treated CE(100ug/ml or CCP(100, 200, and 400ug/ml), with TNF-α 10 ng/ 12hour. MCP-1 level was measured by ELISA kit. The result were presented by the mean±standard error of mean from three independent experiments(Significance of results, *; p<0.05, **; p<0.01, ***; p<0.001 compared to control, ⁺⁺⁺; p<0.001 compared to normal).

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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