[논문]국내 배나무 꽃에서 분리한 Aureobasidium pullulans MHAU2101의 화상병 발생 억제 효과

오현석; 최효원; 이용환; 이승엽; 이미현; 오상근

doi:10.4489/kjm.20220035

국내 배나무 꽃에서 분리한 Aureobasidium pullulans MHAU2101의 화상병 발생 억제 효과
Inhibitory Effects of Aureobasidium pullulans MHAU2101 Isolated from Domestic Pear Blossom Against Fire Blight 원문보기

한국균학회지 = The Korean journal of mycology, v.50 no.4, 2022년, pp.331 - 341

오현석 (농촌진흥청 국립농업과학원 작물보호과) , 최효원 (농촌진흥청 국립농업과학원 작물보호과) , 이용환 (농촌진흥청 국립농업과학원 작물보호과) , 이승엽 (농촌진흥청 국립농업과학원 작물보호과) , 이미현 (농촌진흥청 국립농업과학원 작물보호과) , 오상근 (충남대학교 농업생명과학대학 응용생물학과)

초록
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본 연구는 국내 배나무 꽃에서 분리한 효모의 형태학적 특성 및 유전자 염기서열 분석을 통해 종을 동정하고, 사과와 배의 미숙과와 꽃사과의 꽃을 대상으로 화상병 발병 억제효과를 확인하고자 수행하였다. 효모의 형태적 특성을 조사한 결과 기존 A. pullulans의 특성과 일치하였으며, large-subunit (LSU) 26S ribosomal DNA의 D1/D2 영역과 internal transcribed spacer (ITS) 영역의 염기서열을 분석한 결과 A. pullulans (MHAU2101)로 동정되었다. E. amylovora를 미숙과에 접종하기 전에A. pullulans MHAU2101을 처리할 경우 사과와 배의 미숙과에서 모두 화상병 병징이 나타나지 않았다. 꽃사과 꽃에서 화상병 방제 효과를 검정한 결과, A. pullulans MHAU2101 처리구는 무처리구에 비해 꽃 감염률이 유의하게 낮았으며, 스트렙토마이신 수화제 대비 64%의 방제효과를 보였다. 또한A. pullulans MHAU2101 처리구는 무처리구와 비교하여 암술과 화통 모두E. amylovora의 밀도가 낮았으며 특히 화통에서 밀도가 낮은 특징이 있었다. 따라서 본 연구 결과를 통해 국내 배나무 꽃에서 분리한A. pullulans MHAU2101이 화상병 발병을 억제하는데 효과가 있음을 확인하였다.

Abstract ▼ AI-Helper

This study was conducted to identify yeast species isolated from domestic pear blossom through gene sequencing and analysis of morphological characteristics, and to confirm specific yeast species inhibitory effects toward fire blight in immature apples, pears, and crab apple blossoms. Yeast morphological characteristics were consistent with the known characteristics of Aureobasidium pullulans. Nucleotide sequencing of the D1/D2 region of large-subunit (LSU) 26S ribosomal DNA and the internal transcribed spacer (ITS) region confirmed its identity as A. pullulans (MHAU2101). Inoculation of immature fruits with A. pullulans MHAU2101 before exposure to Erwinia amylovora prevented fire blight symptoms in apples and pears. A. pullulans MHAU2101 treated crab apple blossoms had a significantly lower flower infection rate than untreated blossoms, revealing 64% of the potency of streptomycin. The A. pullulans MHAU2101 treated group also displayed lower E. amylovora density in both pistil and hypanthium compared to the untreated group, especially in the hypanthium. This study confirms that A. pullulans MHAU2101 isolated from domestic pear blossom can effectively suppress the onset of fire blight.

주제어

표/그림 (7)

그림 Fig. 1. Morphological characteristics of Aureobasidium pullulans MHAU2101. Colony of the yeast isolate grown for 14 days on potato dextrose agar medium (A), melanised hyphae and conidia, scale bar=50 μm (B), production of blastospores from the swollen cell, scale bar=10 μm (C).
그림 Fig. 2. Phylogenetic tree of yeast isolated from domestic pear blossom, based on the nucleotide sequences of large-subunit 26S ribosomal DNA D1/D2 region. The tree was generated by the Maximum-likelihood method, using MEGA. Red box: Aureobasidium pullulans MHAU2101.
그림 Fig. 3. Symptoms of fire blight in immature fruits 7 days after inoculation of Erwinia amylovora TS3128. Apple (A), pear (B), hole treated with Aureobasidium pullulans MHAU2101 (+) or distilled water (-) 2 days before inoculation of Erwinia amylovora .
그림 Fig. 4. Symptoms of blossom blight in crab apple 5 days after inoculation of Erwinia amylovora TS3128. Treatment of streptomycin on blossoms 1 day after inoculation of Erwinia amylovora TS3128 (A), treatment of Aureobasidium pullulans MHAU2101 on blossoms 2 days before inoculation of Erwinia amylovora TS3128 (B), water control (C).
표 Table 1. Control effect of Aureobasidium pullulans MHAU2101 and streptomycin for fower infection of crab apple caused by Erwinia amylovora TS3128.
표 Table 2. The mean cycle threshold (Ct) end-point fuorescence of 10-fold serial dilutions of Erwinia amylovora TS3128 with real time PCR assay.
표 Table 3. In real time PCR assay, A cycle threshold (Ct) of Erwinia amylovora TS3128 by fower part according to the treatment of Aureobasidium pullulans MH2101.

참고문헌 (26)

Sundin GW, Werner NA, Yoder KS, Aldwinckle HS. Field evaluation of biological control of？fire blight in the eastern United States. Plant Dis 2009;93:386-94.

상세보기
Park DH, Yu JG, Oh EJ, Han KS, Yea MC, Lee SJ, Myung IS, Shim HS, Oh CS. First？report of fire blight disease on Asian pear caused by Erwinia amylovora in Korea. Plant Dis？2016;100:1946.
Ham HH, Lee KJ, Hong SJ, Kong HG, Lee MH, Kim HR, Lee YH. Outbreak of fire blight of？apple and pear and its characteristics in Korea in 2019. Res Plant Dis 2020;26:239-49.
Norelli JL, Jones AL, Aldwinckle HS. Fire blight management in the twenty-first century:？using new technologies that enhance host resistance in apple. Plant Dis 2003;87:756-65.

상세보기
Jones AL, Schnabel EL. Streptomycin and oxytetracycline resistance determinants？detected among bacteria from Michigan apple orchards and their potential importance. VIII？International Workshop on Fire Blight 1998;489:673-76.
Vidaver AK. Uses of antimicrobials in plant agriculture. Clin Infect Dis 2002;34:S107-10.

상세보기
Slack SM, Outwater CA, Grieshop MJ, Sundin GW. Evaluation of a contact sterilant？as a niche-clearing method to enhance the colonization of apple flowers and efficacy of？Aureobasidium pullulans in the biological control of fire blight. Biol Control 2019;139:104073.

상세보기
Chi Z, Wang F, Chi Z, Yue L, Liu G, Zhang T. Bioproducts from Aureobasidium pullulans, a？biotechnologically important yeast. Appl Microbiol Biotechnol 2009;82:793-804.

상세보기
Loncaric I, Donat C, Antlinger B, Oberlerchner JT, Heissenberger B, Moosbeckhofer R.？Strain-specific detection of two Aureobasidium pullulans strains, fungal biocontrol agents of？fire blight by new, developed multiplex-PCR. J Appl Microbiol 2008;104:1433-41.

상세보기
Leibinger W, Breuker B, Hahn M, Mendgen K. Control of postharvest pathogens and？colonization of the apple surface by antagonistic microorganisms in the field. Phytopathology？1997;87:1103-10.

상세보기
Kunz S. Development of "Blossom-protect"-a yeast preparation for the reduction of？blossom infections by fire blight. 11th International Conference on Cultivation Technique？and Phytopathological Problems in Organic Fruit-Growing; 2004 February 3-5. Weinsberg:？Ecofruit; 2004. p. 108-12.
Kurtzman CP, Robnett C. Identification of clinically important ascomycetous yeasts based？on nucleotide divergence in the 5'end of the large-subunit (26S) ribosomal DNA gene. J Clin？Microbiol 1997;35:1216-23.

상세보기
White TJ, Bruns T, Lee S, Taylor J, White TJ, Bruns T, Lee S, Taylor J. Amplification and？direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a？guide to methods and applications. New York: Academic Press; 1990. p. 315-22.
Li Y, Chi Z, Wang GY, Wang ZP, Liu GL, Lee CF, Ma ZC, Chi ZM. Taxonomy of？Aureobasidium spp. and biosynthesis and regulation of their extracellular polymers. Crit Rev？Microbiol 2015;41:228-37.
Zalar P, Gostincar C, de Hoog GS, Ursic V, Sudhadham M, Gunde-Cimerman N. Redefinition？of Aureobasidium pullulans and its varieties. Stud Mycol 2008;61:21-38.
Kim JS, Lee M, Song MY, Kwon SW, Kim SJ, Hong SB, Park BY, Yun BS. Isolation and？identification of Aureobasidium spp. from flowers of the jeolla-do province in Korea. Kor J？Mycol 2018;46:415-25.
Joshi NK, Ngugi HK, Biddinger DJ. Bee vectoring: development of the Japanese orchard？bee as a targeted delivery system of biological control agents for fire blight management.？Pathogens 2020;9:41.

상세보기
Mari M, Martini C, Spadoni A, Rouissi W, Bertolini P. Biocontrol of apple postharvest decay？by Aureobasidium pullulans. Postharvest Biol Technol 2012;73:56-62.

상세보기
Bencheqroun SK, Bajji M, Massart S, Labhilili M, Jaafari SE, Jijakli MH. In vitro and in situ？study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: evidence for the？involvement of competition for nutrients. Postharvest Biol Technol 2007;46:128-35.

상세보기
EFSA (European Food Safety Authority). Potential inclusion of the microorganism？Aureobasidium pullulans strains DSM 14940 and DSM 14941 in annex IV of Regulation (EC)？No 369/2005. EFSA Journal 2011;9:2435.
Johnson KB, Temple TN. Evaluation of strategies for fire blight control in organic pome fruit？without antibiotics. Plant Dis 2013;97:402-9.

상세보기
Duffy B, Vogelsanger J, Schoch B, Holliger E. Biocontrol of Erwinia amylovora using a？commercial yeast strain mixture. Acta Hortic 2006;704:363.

상세보기
Temple TN, Thompson EC, Uppala S, Granatstein D, Johnson KB. Floral colonization？dynamics and specificity of Aureobasidium pullulans strains used to suppress fire blight of？pome fruit. Plant Dis 2020;104:121-8.

상세보기
Dreo T, Pirc M, Ravnikar M. Real-time PCR, a method fit for detection and quantification of？Erwinia amylovora. Trees 2012;26:165-78.

상세보기
Lee MS, Lee I, Kim SK, Oh CS, Park DH. In vitro screening of antibacterial agents for？suppression of fire blight disease in Korea. Res Plant Dis 2018;24:41-51.
Pusey PL, Stockwell VO, Mazzola M. Epiphytic bacteria and yeasts on apple blossoms and？their potential as antagonists of Erwinia amylovora. Phytopathology 2009;99:571-81.

상세보기

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