[논문]배양방법과 배지성분이 시루송편버섯 균사체 매트 형성에 미치는 영향

정용현; 김다송; 김현석; 오득실; 신현재

doi:10.14480/jm.2022.20.2.69

[국내논문] 배양방법과 배지성분이 시루송편버섯 균사체 매트 형성에 미치는 영향
Effect of culture method and medium components on Trametes orientalis mycelium mat formation (Yasuda) Imazeki 원문보기

Journal of mushrooms = 한국버섯학회지, v.20 no.2, 2022년, pp.69 - 77

정용현 (조선대학교 대학원 화학공학과) , 김다송 (조선대학교 대학원 화학공학과) , 김현석 (전라남도산림자원연구소) , 오득실 (전라남도산림자원연구소) , 신현재 (조선대학교 대학원 화학공학과)

초록
AI-Helper

기존에 사용되는 1차 봉지배양 및 2차 매트생산 배양에 소요되는 기간을 단축시키기 위해 시루송편버섯 균사체에 Co-60 저준위 감마선 200 Gy 24시간 조사로 돌연변이 시킨 ToM 균사체를 활용하여 봉지배양이 생략된 박스 배양법을 시도하였다. 또한, 매트의 품질 및 물성을 강화시키기 위해 균액으로 ammonium nitrate가 3 g/L첨가된 mYMB6을 사용하였고, CaCO₃를 1.5% 함유한 톱밥배지에서 균일한 품질의 균사체 매트 생산이 가능하다는 점을 확인하였으며, 기간 또한 기존의 생산방법과 비교하여 10일 이상 단축할 수 있다. 본 결과는 균사체 기반 친환경 소재 연구에 활용할 수 있을 것으로 생각된다.

Abstract ▼ AI-Helper

Eco-friendly materials, such as alternative vegan materials using various fungal resources, are being actively researched to reduce environmental pollution and facilitate a healthy lifestyle. The fungal mycelium-based mushroom mycelium mat is one such emerging material. In this study, the commonly used mushroom mycelium culture method was modified to reduce the time required to produce the mycelium mat, lower the possibility of contamination, and improve the properties and quality of the mat. Shortening the period required for the previously used primary bag culture and secondary mat production culture. A culture method in which the bag culture was omitted was attempted using a mycelium mutated by gamma irradiation to the mycelium of Trametes orientalis. In addition, various nutrients were added to the fungal solution to observe the change in physical properties of the fungal mat. High-quality mycelium mats were produced in the experimental group containing 1.5% CaCO3 in sawdust medium, and the period was also reduced by more than 10 days compared to the existing production method. In the future, for mass producing mycelium mats, additional selection of medium components and optimization of culture conditions are essential.

주제어

표/그림 (12)

그림 Fig. 1. Schematic diagram of the procedure about box cultivation.
표 Table 1. Composition of a nitrogen enriched medium for culturing T. orientalis mutation
그림 Fig. 2. Growth curves of ToM cultured in shaking incubator for 8 days at 27oC and 150 rpm.
표 Table 2. Characteristics of ToM in nitrogen enriched medium by temperature
그림 Fig. 3. Characteristics of ToM in nitrogen enriched medium by temperature.
그림 Fig. 4. Characteristics of ToM in nitrogen enriched medium by box cultivation.
그림 Fig. 5. Surface of mycelium mat in media containing (A) CaCO₃ and (B) N-acetyl glucosamine.
표 Table 3. Mycelium mat properties by culture method
표 Table 4. Properties of mycelium mat in media containing CaCO₃ and N-acetyl glucosamine
그림 Fig. 6. Tensile strength (N/mm²) of mycelium mat from reinforced substrate containing rate 0.5% (black bars), 1.0% (light gray bars), 1.5% (dark gray bars) CaCO₃ and N-acetyl glucosamine (N-ag). The reinforced media mYMB3 and mYMB6 were used for each substrate. *Indicates statistical significant difference compared to the control (Con; white bar) (p < 0.05).
그림 Fig. 7. Elongation rate (%) of mycelium mat from reinforced substrate containing rate 0.5% (black bars), 1.0% (light gray bars), 1.5% (dark gray bars) CaCO₃ and N-acetyl glucosamine (N-ag). The reinforced media mYMB3 and mYMB6 were used for each substrate. *Indicates statistical significant difference compared to the control (Con; white bar) (p < 0.05).
그림 Fig. 8. Density (g/cm³) of mycelium mat from reinforced substrate containing rate 0.5% (black bars), 1.0% (light gray bars), 1.5% (dark gray bars) CaCO₃ and N-acetyl glucosamine (N-ag). The reinforced media mYMB3 and mYMB6 were used for each substrate. *Indicates statistical significant difference compared to the control (Con; white bar) (p < 0.05).

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