[논문]Cryopreservation of Siberian tiger (Panthera tigris altaica) epididymal spermatozoa: pilot study of post-thaw sperm characteristics

Ibrahim, Saddah; Talha, Nabeel Abdelbagi Hamad; Kim, Jeongho; Jeon, Yubeol; Yu, Iljeoung

doi:10.12750/jarb.37.2.130

Cryopreservation of Siberian tiger (Panthera tigris altaica) epididymal spermatozoa: pilot study of post-thaw sperm characteristics 원문보기

Journal of animal reproduction and biotechnology = 한국동물생명공학회지, v.37 no.2, 2022년, pp.130 - 135

Ibrahim, Saddah (Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-safety Research Institute, Jeonbuk National University) , Talha, Nabeel Abdelbagi Hamad (Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-safety Research Institute, Jeonbuk National University) , Kim, Jeongho (Cheongju Zoo) , Jeon, Yubeol (Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-safety Research Institute, Jeonbuk National University) , Yu, Iljeoung (Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-safety Research Institute, Jeonbuk National University)

Abstract ▼ AI-Helper

Epididymal sperm cryopreservation provides a potential method for preserving genetic material from males of endangered species. This pilot study was conducted to develop a freezing method for tiger epididymal sperm. We evaluated post-thaw sperm condition using testes with intact epididymides obtained from a Siberian tiger (Panthera tigris altaica) after castration. The epididymis was chopped in Tyrode's albumin-lactate-pyruvate 1x and incubated at 5% CO2, 95% air for 10 min. The Percoll separation density gradient method was used for selective recovery of motile spermatozoa after sperm collection using a cell strainer. The spermatozoa were diluted with modified Norwegian extender supplemented with 20 mM trehalose (extender 1) and subsequent extender 2 (extender 1 with 10% glycerol) and frozen using LN2 vapor. After thawing at 37℃ for 25 s, Isolate® solution was used for more effective recovery of live sperm. Sperm motility (computerized assisted sperm analysis, CASA), viability (SYBR-14 and Propidium Iodide) and acrosome integrity (Pisum sativum agglutinin with FITC) were evaluated. The motility of tiger epididymal spermatozoa was 40.1 ± 2.0%, and progressively motile sperm comprised 32.7 ± 2.3%. Viability was 56.3 ± 1.6% and acrosome integrity was 62.3 ± 4.4%. Cryopreservation of tiger epididymal sperm using a modified Norwegian extender and density gradient method could be effective to obtain functional spermatozoa for future assisted reproductive practices in endangered species.

주제어

표/그림 (3)

그림 Fig. 1. Testes of Siberian tiger.
표 Table 1. Post-thaw motility of Siberian tiger epidydimal spermatozoa cryopreserved with modified Norwegian extender
그림 Fig. 2. Post-thaw viability and acrosome integrity of Siberian tiger epidydimal spermatozoa cryopreserved with modified Norwegian extender. (A) Live spermatozoa stained with SYBR® 14. Magnification at 100x. (B) Dead spermatozoa stained with propidium iodide. Magnification at 100x. Arrow indicates artifacts. (C) Intact acrosome of spermatozoa stained with Pisum Sativum Agglutinin labelled with FITC. (D) A graph shows the results of viability and acrosome integrity. Values are expressed as mean ± SE.

참고문헌 (16)

Almubarak AM, Kim W, Abdelbagi NH, Balla SE, Yu IJ, Jeon Y. 2021. Washing solution and centrifugation affect kinematics of cryopreserved boar semen. J. Anim. Reprod. Biotechnol. 36:65-75.
Assumpcao TID, Severo NC, Zandonaide JPB, Macedo GG. 2021. Magnetic-activated cell sorting improves high-quality spermatozoa in bovine semen. J. Anim. Reprod. Biotechnol. 36:91-98.

원문보기 상세보기
Byers AP, Hunter AG, Seal US, Binczik GA, Graham EF, Reindl NJ, Tilson RL. 1989. In-vitro induction of capacitation of fresh and frozen spermatozoa of the Siberian tiger (Panthera tigris). J. Reprod. Fertil. 86:599-607.

상세보기
Cocchia N, Ciani F, El-Rass R, Russo M, Borzacchiello G, Esposito V, Montagnaro S, Avallone L, Tortora G, Lorizio R. 2010. Cryopreservation of feline epididymal spermatozoa from dead and alive animals and its use in assisted reproduction. Zygote 18:1-8.

상세보기
Donoghue AM, Johnston LA, Seal US, Armstrong DL, Simmons LG, Gross T, Tilson RL, Wildt DE. 1992. Ability of thawed tiger (Panthera tigris) spermatozoa to fertilize conspecific eggs and bind and penetrate domestic cat eggs in vitro. J. Reprod. Fertil. 96:555-564.

상세보기
Filliers M, Rijsselaere T, Bossaert P, De Causmaecker V, Dewulf J, Pope CE, Van Soom A. 2008. Computer-assisted sperm analysis of fresh epididymal cat spermatozoa and the impact of cool storage (4 degrees C) on sperm quality. Theriogenology 70:1550-1559.
Kang SS, Kim UH, Ahn JS, Won JI, Cho SR. 2021. Improvement of pregnancy rate after deep uterine artificial insemination with frozen-thawed cauda epididymal spermatozoa in Hanwoo cattle. J. Anim. Reprod. Biotechnol. 36:82-90.

원문보기 상세보기
Kang SS, Kim UH, Lee MS, Lee SD, Cho SR. 2020. Spermatozoa motility, viability, acrosome integrity, mitochondrial membrane potential and plasma membrane integrity in 0.25 mL and 0.5 mL straw after frozen-thawing in Hanwoo bull. J. Anim. Reprod. Biotechnol. 35:307-314.

원문보기 상세보기
Karja NWK, Fahrudin M, Setiadi MA, Tumbelaka LI, Sudarwati R, Hastuti YT, Mulia BH, Widianti A, Sultan K, Terazono T, Namula Z, Taniguchi M, Tanihara F, Takemoto T, Kikuchi K, Sato Y, Otoi T. 2016. Characteristics and fertility of Sumatran tiger spermatozoa cryopreserved with different sugars. Cryo Letters 37:264-271.
Kim SW, Lee JY, Kim CL, Ko YG, Kim B. 2022. Evaluation of rooster semen quality using CBB dye based staining method. J. Anim. Reprod. Biotechnol. 37:55-61.

원문보기 상세보기
Mathers MJ, Degener S, Sperling H, Roth S. 2017. Hematospermia-a symptom with many possible causes. Dtsch. Arztebl. Int. 114:186-191.
Nabeel AHT, Jeon Y, Yu IJ. 2019. Use of polyvinyl alcohol as a chemically defined compound in egg yolk-free extender for dog sperm cryopreservation. Reprod. Domest. Anim. 54: 1449-1458.

상세보기
Patil SB, Jayaprakash D, Shivaji S. 1998. Cryopreservation of semen of tigers and lions: computerized analysis of the motility parameters of the spermatozoa. Curr. Sci. 75:930-935.
Schleh C and Leoni AL. 2013. How to optimize the benefits of computer assisted sperm analysis in experimental toxicology. J. Occup. Med. Toxicol. 8:6.

상세보기
Yu I, Songsasen N, Godke RA, Leibo SP. 2002. Differences among dogs in response of their spermatozoa to cryopreservation using various cooling and warming rates. Cryobiology 44:62-78.

상세보기
Yu IJ. 2014. Canine sperm cryopreservation using glucose in glycerol-free Tris. Cryo Letters 35:101-107.

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Cryopreservation of Siberian tiger (Panthera tigris altaica) epididymal spermatozoa: pilot study of post-thaw sperm characteristics 원문보기

Abstract ▼ AI-Helper

주제어

표/그림 (3)

표/그림 (3)

참고문헌 (16)

이 논문을 인용한 문헌

관련 콘텐츠

원문 보기

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트