[논문]Streptococcus sp. 배양 여과물(HAIRCLETM)의 모발 개선 효과

박혜림; 김하연; 김진영; 오신석; 강필선; 이강혁; 임정혁; 신송석

doi:10.15230/scsk.2022.48.2.169

Streptococcus sp. 배양 여과물(HAIRCLETM)의 모발 개선 효과
Hair Revitalization Effects of Streptococcus sp. Strain Culture Medium Filtrate (HAIRCLETM) 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.48 no.2, 2022년, pp.169 - 180

박혜림 (현대바이오랜드 생명과학연구소) , 김하연 (현대바이오랜드 생명과학연구소) , 김진영 (현대바이오랜드 생명과학연구소) , 오신석 (한국교통대학교 응용화학에너지공학부) , 강필선 (한국교통대학교 응용화학에너지공학부) , 이강혁 (현대바이오랜드 생명과학연구소) , 임정혁 (한국교통대학교 응용화학에너지공학부) , 신송석 (현대바이오랜드 생명과학연구소)

초록
AI-Helper

Streptococcus sp.는 일반적으로 히알루론산을 생산하는 균주로 알려져 있다. 본 연구에서는 Streptococcus 균주가 배양하면서 만들어내는 여러 대사산물 중 히알루론산 이외의 영양성분에 주목하였다. 히알루론산이 생성되지 않은 조건에서의 Streptococcus sp. 배양 여과물로 모발 실험용 샘플(HAIRCLE^TM)을 제조하고 모발에 미치는 효과를 확인하였다. 모발의 인장강도는 만능시험기(universal testing machine, UTM)로 측정하였으며, 모발표면의 미세구조는 atomic force microscope (AFM)으로 분석하였다. 이 연구를 통해 Streptococcus sp. 배양 여과물은 손상된 모표피개선, 마찰력증가 및 모발강도의 증가 등의 모발개선 효과가 있는 것으로 확인되었다. 또한 in vitro 모발관련 실험을 통해 Streptococcus sp. 배양 여과물이 모발성장인자인 혈관내피세포 성장인자(vascular endothelial growth factor, VEGF), 간세포성장인자(hepatocyte growth factor, HGF), Wnt10b의 발현 촉진 효과가 있음을 확인 하였으며, 안드로젠 수용체(androgen receptor, AR)와 TGF-β2 발현을 저해시켜 남성형 탈모완화 효과도 있음을 확인 하였다. 또한 산화스트레스에 대해 장벽 관련 인자(INV, CLDN-1)의 발현을 증가시키고, 염증성 인자인 COX-2 발현을 억제시킴으로써 손상된 두피 장벽을 개선하고 유도된 염증을 완화시키는 효능이 있음을 확인하였다.

Abstract ▼ AI-Helper

Streptococcus sp. is generally known as a strain that produces hyaluronic acid. In this study, we focused on ingredients other than hyaluronic acid among several metabolites produced by Streptococcus sp. during cultivation. The hair experimental sample (HAIRCLETM) was prepared with Streptococcus sp. culture filtrate under the condition that hyaluronic acid was not produced and the effect on the hair was identified. Tensile strength of hair was tested with a universal testing machine (UTM). Surface properties of a hair were examined with an atomic force microscope (AFM). Our results suggest that HAIRCLETM could prevent damage to a hair such as destroyed-cuticle, increased-faction force, and broken-hair. Furthermore, hair-related experiments confirmed that HAIRCLETM could promote the expression of hair growth factors VEGF, HGF, and Wnt10b and mitigate male hair loss by inhibiting androgen receptors and TGF-β2 expression. In addition, it was confirmed that the expression of barrier-related factors (INV, CLDN-1) was increased for oxidative stress, and the COX-2 expression, an inflammatory factor, was inhibited, thereby improving damaged scalp barriers and alleviating induced inflammation.

주제어

표/그림 (16)

표 Table 1. Summary of Hair Samples Examined in the Experiment
표 Table 2. Primer Sequences
표 Table 3. Primer Sequences
표 Table 4. Primer Sequences
표 Table 5. Primer Sequences
표 Table 6. Total Amino Acid Analysis of HAIRCLE^TM
그림 Figure 1. AFM topography images of hair surface. (A) sample 1, (B) sample 2, (C) sample 3, (D) sample 4.
표 Table 7. Tensile Strength Changes of Hair
그림 Figure 2. A FM 3 D Images o f hair s urface a nd l ine profile o f height. (A) Sample 1 (30 μm × 30 μm). (B) Sample 2 (30 μm × 30 μm).
그림 Figure 3. Roughness (Ra, nm) graph of hair surface of Sample 1 and 2.
그림 Figure 4. Friction force graph of hair surface for Sample 1, 2, 3 and 4.
그림 Figure 5. Cell viability test of HAIRCLE™ by hDPC, HHGMC and NHEKs. (A) DPC (B) HHGMC and (C) NHEKs were treated with various concentrations (0.1 to 5%) of control for 24 h. The data are expressed as the mean value (± SD) from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.
그림 Figure 6. Hair bulb strength effect of HAIRCLE™ by hDPC. hDPC treated with concentration 0.1% to 0.5% samples for 24h. The mRNA expression of HGF and VEGF were determined by real-time PCR. The data are expressed as the mean value (± SD) from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.
그림 Figure 7. Hair growth effects of HA IRCLE^TM by HHGMC. HHGMC treated with concentration 0.1% to 0.5% HAIRCLE^TM for 24 h. The mRNA expression of Wnt10b was determined by real-time PCR. The data is expressed as the mean value (± SD) from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.
그림 Figure 8. Androgen alopecia inhibit effects of HAIRCLE^TM by hDPC. hDPC treated with 50 nM DHT and added concentration with 0.1% to 0.5% HAIRCLE^TM for 48 h. The mRNA expression of AR and TGF-β2 were determined by real-time PCR. The data are expressed as the mean value (± SD) from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.
그림 Figure 9. Scalp barrier improvement and soothing against H₂O₂ effects of HAIRCLE^TM by NHEKs. NHEKs treated with H₂O₂ and added concentration 1% to 4% HAIRCLE^TM for 24 h. The mRNA expression of involucrin, claudin-1 and COX-2 were determined by real-time PCR. The data are expressed as the mean value (± SD) from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.

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