[논문]저온 저장 감수성 및 저항성 고구마 품종에서 저온 반응성 단백질체 연구

김윤희; 지창윤; 김호수; 정정성; 최성환; 곽상수; 이증주

doi:10.5010/jpb.2022.49.2.118

[국내논문] 저온 저장 감수성 및 저항성 고구마 품종에서 저온 반응성 단백질체 연구
Proteome analysis of storage roots of two sweet potato cultivars with contrasting low temperature tolerance during storage 원문보기

Journal of plant biotechnology = 식물생명공학회지, v.49 no.2, 2022년, pp.118 - 123

김윤희 (경상국립대학교 사범대학 생물교육과(농업생명과학연구원)) , 지창윤 (한국생명공학연구원 식물시스템공학연구센터) , 김호수 (한국생명공학연구원 식물시스템공학연구센터) , 정정성 (경상국립대학교 농업생명과학대학 농학과(농업생명과학연구원)) , 최성환 (경상국립대학교 농업생명과학대학 원예과학부(농업생명과학연구원)) , 곽상수 (한국생명공학연구원 식물시스템공학연구센터) , 이증주 (경상국립대학교 농업생명과학대학 식물의학과(농업생명과학연구원))

초록
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고구마의 저온 내성 반응의 분자적 기작에 대한 정보를 얻기 위해 저온 저장 내성이 높은 고구마 품종 Xushu 15-1과 저온 저장 내성이 낮은 Xushu 15-4에서 발현되는 단백질체를 2-D와 MALDI-TOF/TOF를 이용하여 분석하였다. 저온 처리가 되지 않은 대조구 간의 분석 결과, Xushu 15-1에서는 4개의 단백질 spot이 새롭게 발현되었으며, Xushu 15-4에서는 1개 단백질 spot의 발현량이 Xushu 15-1에 비하여 더 높았다. 이들 중 Xushu 15-1에서 새롭게 발현된 spot 2는 sporamin으로 동정되었다. 4℃ 처리된 2개 품종의 저장뿌리에서 8주 동안의 단백질 발현량의 변화를 조사한 결과, Xushu 15-1에서는 별다른 차이가 없었다. 그러나, Xushu 15-4에서는 1개 단백질 spot의 발현량이 증가하였고 4개 spot들의 발현량은 감소하였다. 발현량이 감소된 단백질 중 spot 7 및 8번은 actin, spot 9 및 10번은 fructokinase-like protein으로 동정되었다. 본 연구의 결과는 저장 중 고구마의 저온 내성과 관련된 복잡한 반응성 기작에 대한 이해를 높이고, 향후 저온 저장 능력이 향상된 신품종 고구마의 개발을 위한 후보 유전자를 특정하는 데 이용될 수 있을 것으로 사료된다.

Abstract ▼ AI-Helper

To obtain information on the molecular mechanism underlying the low temperature tolerance of sweet potato [Ipomoea batatas (L.) Lam], the proteome expressed in the sweet potato cultivar Xushu 15-1 with high cold storage tolerance and in the cultivar Xushu 15-4 with low cold storage tolerance was analyzed using 2-D and MALDI-TOF/TOF analyses. Compared with the control (without cold treatment), four protein spots were newly expressed in Xushu 15-1. The expression level of one protein spot was higher in Xushu 15-4 than in Xushu 15-1. Spot 2, which was newly expressed in Xushu 15-1, was identified as sporamin. Assessment of the change in protein expression levels over 8 weeks in the storage roots of the two cultivars treated at 4℃ revealed no significant difference in the expression levels in Xushu 15-1 over time. However, in Xushu 15-4, the expression level of one protein spot increased, while those of four spots decreased. Of the proteins with reduced expression levels, spots 7 and 8 were identified as actin and spots 9 and 10 were identified as fructokinase-like proteins. The present results are expected to enhance the understanding of the complex mechanism underlying the low temperature tolerance of sweet potatoes during storage and can be used to identify candidate genes for the development of new varieties of sweet potatoes with improved low temperature tolerance during cold storage in the future.

주제어

표/그림 (4)

그림 Fig. 1 High-resolution 2-D analysis of total protein extracted from storage roots of two cultivars of sweet potatoes with contrasting low temperature tolerance during storage. The proteins were first separated on IPG strips (pH 4-7), following which SDS-PAGE was performed on 13.0% polyacrylamide gels. Gels were stained with colloidal CBB. Spot numbers indicate proteins that were differentially expressed in Xushu 15-1 (with high cold storage tolerance) and Xushu 15-4 (with low cold storage tolerance). Red arrows indicate spots expressed in both Xushu 15-1 and Xushu 15-4 during low temperature storage, while black arrows indicate spots specific to Xushu 15-4 during low temperature storage. Ten gels per biological replicate were assessed
그림 Fig 2 Enlarged views of 2-D maps of differentially expressed proteins marked in Fig. 1. (A) Spots indicated by red arrows in Fig. 1. (B) Spots indicated by black arrows in Fig. 1
그림 Fig. 3 Transcriptome-based DEG analysis of genes encoding sporamin, actin, and fructokinase. A heatmap was constructed using MultiExperiment Viewer (MeV)
표 Table 1 Differentially expressed in Xushu 15-1 and/or Xushu 15-4 sweetpotato lines, identified by MALDI-TOF MS analysis

참고문헌 (38)

Andrade Jde M, Toledo TT, Nogueira SB, Cordenunsi BR, Lajolo FM, do Nascimento JR (2012) 2D-DIGE analysis of mango (Mangifera indica L.) fruit reveals major proteomic changes associated with ripening. J Proteomics 75:3331-3341
Bovell-Benjamin AC (2007) Sweetpotato: a review of its past, present, and future role in human nutrition. Adv Food Nutr Res 52:1-59

상세보기
Bradford MM (1976) A rapid sensitive methods for the quantization of microgram quantities of protein utilizing the principles of protein-dye binding. Anal Biochem 72:248-255
Chen S, Harmon AC (2006) Advances in plant proteomics. Proteomics 6:5504-5516

상세보기
Damari-Weissler H, Rachamilevitch S, Aloni R, German MA, Cohen S, Zwieniecki MA, Holbrook NM, Granot D (2009) LeFRK2 is required for phloem and xylem differentiation and the transport of both sugar and water. Planta 230:795-805

상세보기
Dennis D, Blakeley S (2000) Carbohydrate metabolism. Biochem Mol Biol Plant 2000:630-675
Eckert R, Randall D, Burggren WW, French K (2002) Eckert animal physiology: mechanisms and adaptations. New York: W.H. Freeman and CO.
Edmunds B, Boyette M, Clark C, Ferrin D, Smith T, Holmes G (2008) Postharvest handling of sweetpotato. North Carolina Coop Ext Serv.
German MA, Dai N, Matsevitz T, Hanael R, Petreikov M, Bernstein N, Ioffe M, Shahak Y, Schaffer AA, Granot D (2003) Suppression of fructokinase encoded by LeFRK2 in tomato stem inhibits growth and causes wilting of young leaves. Plant J 34:837-846

상세보기
Grummt I (2006) Actin and myosin as transcription factors. Curr Opin Gene Dev 16:191-196
Gunning PW, Ghoshdastider U, Whitaker S, Popp D, Robinson RC (2015) The evolution of compositionally and functionally distinct actin filaments. J Cell Sci 128:2009-2019

상세보기
Ha J, Won JC, Jung YH, Yang JW, Lee HU, Nam KJ, Park SC, Jeong JC, Lee SW, Lee DW, Chung JS, Lee JJ, Kim YH (2017) Comparative proteomic analysis of the response of fibrous roots of nematode-resistant and -sensitive sweet potato cultivars to root-knot nematode Meloidogyne incognita. Acta Physiol Plant 39:262

상세보기
Hattori T, Yoshida N, Nakamura K (1989) Structural relationship among the members of multigene family coding for the sweetpotato tuberous roots storage proteins. Plant Mol Biol 13:563-572
Higaki T, Sano T, Hasezawa S (2007) Actin microfilament dynamics and actin side-binding proteins in plants. Curr Opin Plant Biol 10:549-556
Hurkman WJ, Tanaka CK (1986) Solubilization of plant membrane proteins for analysis by to-dimensional gel electrophoresis. Plant Physiol 81:802-806
Ji CY, Chung WH, Kim HS, Jung WY, Kang L, Jeong JC, Kwak SS (2017a) Transcriptome profiling of sweetpotato tuberous roots during low temperature storage. Plant Physiol Biochem 112:97-108

상세보기
Ji CY, Jin R, Xu Z, Kim HS, Lee CJ, Kang L, Kim SE, Lee HU, Lee JS, Kang CH, Chi YH, Lee SY, Xie Y, Li H, Ma D, Kwak SS (2017b) Overexpression of Arabidopsis P3B increases heat and low temperature stress tolerance in transgenic sweetpotato. BMC Plant Biol 17:139

상세보기
Ji CY, Kim HS, Lee CJ, Kim SE, Lee HU, Nam SS, Lie Q, Mae D, Kwak SS (2020) Comparative transcriptome profiling of tuberous roots of two sweetpotato lines with contrasting low temperature tolerance during storage. Gene 727:144244
Kim ST, Kim SG, Hwang DH, Kang SY, Kim HJ, Lee BH, Lee JJ, Kang KY (2004) Proteomic analysis of pathogen-responsive proteins from rice leaves induced by rice blast fungus, Magnaporthe grisea. Proteomics 4:3569-3578.

상세보기
Kwak SS (2019) Biotechnology of the sweetpotato: ensuring global food and nutrition security in the face of climate change. Plant Cell Rep 38:1361-1363

상세보기
Lee JJ, Kim YH, Kwak YS, An JY, Kim PJ, Lee BH, Kumar V, Park K, Chang ES, Jeong JC, Lee HS, Kwak SS (2015) A comparative study of proteomic differences between pencil and storage roots of sweetpotato (Ipomoea batatas (L.) Lam.) Plant Physiol Biochem 87:92-101

상세보기
Lee JJ, Park KW, Kwak YS, Ahn JY, Jung YH, Lee BH, Jeng JC, Lee HS, Kwak SS (2012) Comparative proteomic study between tuberous roots of light orange- and purple-fleshed sweetpotato cultivars. Plant Sci. 19:120-129
Lodish HF, Berk A, Kaiser C, Krieger M, Bretscher A, Ploegh H, amon A, Martin KC (2016) Cell Organization and Movement I: Microfilaments. Molecular Cell Biology (Eighth ed.). New York: W.H. Freeman
Lieberman M, Craft CC, Audia W, Wilcox M (1958) Biochemical studies of chilling injury in sweetpotatoes. Plant Physiol 33:307-311

상세보기
Maeshima M, Sasaki T, Asahi T (1985) Characterization of major proteins in sweet potato tuberous roots. Phytochemistry 24:1899-1902

상세보기
Manaa A, Faurobert M, Valot V, Bouchet JP, Grasselly D, Causse M, Ahmed HB (2013) Effect of salinity and calcium on tomato fruit proteome. OMICS 17:338-352

상세보기
Matsui NM, Smith-Beckerman DM, Epstein LB (1999) Staining of preparative 2-D (2-D proteome analysis protocols). Methods Mol Biol 112:370-311
Minamikawa T, Akazawa T, Uritani I (1961) Mechanism of cold injury in sweet potatoes II. Biochemical mechanism of cold injury with special reference to mitochondrial activities. Plant Cell Physiol 2:301-309
Odanaka S, Bennett AB, Kanayama Y (2002) Distinct physiological roles of fructokinase isozymes revealed by gene-specific suppression of Frk1 and Frk2 expression in tomato. Plant Physiol. 129:1119-1126

상세보기
Palma MJ, Corpas FJ, del Rio LA (2011) Proteomics as an approach to the understanding of the molecular physiology of fruit development and ripening. J Proteomic 74:1230-1243
Renz A, Stitt M (1993) Substrate specificity and product inhibition of different forms of fructokinases and hexokinases in developing potato tubers. Planta 190:166-175
Sevillano L, Sanchez-Ballesta MT, Romojaro F, Flores FB (2009) Physiological, hormonal and molecular mechanisms regulating chilling injury in horticultural species. Postharvest technologies applied to reduce its impact. Sci Food Agri 89: 555-573
Wollfe JA (1992) Sweet Potato: an Untapped Food Resource. Cambridge University Press
Wu Z, Cheng J, Qin C, Hu Z, Yin C, Hu K (2013) Differential proteomic analysis of anthers between cytoplasmic male sterile and maintainer lines in Capsicum annuum L. Int J Mol Sci 14:22982-22996
Xie Z, Zhou Z, Li H, Yu J, Jiang J, Tang Z, Ma D, Zhang B, Han Y, Li Z (2019) High throughput sequencing identifies chilling responsive genes in sweetpotato (Ipomoea batatas Lam.) during storage. Genomics 111:1006-1017

상세보기
Yamaki S, Uritani I (1972) Mechanism of chilling injury in sweet potato VII. Changes in mitochondrial structure during chilling storage. Plant Cell Physiol 13:795-805
Yeh KW, Chen JC, Lin MI, Chen YM, Lin CY (1997) Functional activity of sporamin from sweetpotato (Ipomoea batatas Lam.): a tuber storage protein with trypsin inhibitory activity. Plant Mol Biol 33:565-570

상세보기
Zeng Y, Pan Z, Ding Y, Zhu A, Cao H, Xu Q, Deng X (2011) A proteomic analysis of the chromoplasts isolated from sweet orange fruits [Citrus sinensis (L.) Osbeck]. J Exp Bot 62:5297-5309

상세보기

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