[논문]블랙 초크베리가 HepG2세포에서 콜레스테롤 대사에 미치는 효과

이상길; 김보경

doi:10.9721/kjfst.2022.54.4.398

블랙 초크베리가 HepG2세포에서 콜레스테롤 대사에 미치는 효과
Effects of black chokeberry on cholesterol metabolism in HepG2 cells 원문보기

한국식품과학회지 = Korean journal of food science and technology, v.54 no.4, 2022년, pp.398 - 402

이상길 (부경대학교 식품영양학과) , 김보경 (부산대학교 식품영양학과)

초록
AI-Helper

본 연구에서는 폴리페놀 함유 블랙 초크베리가 콜레스테롤 대사에 미치는 영향을 HepG2 세포에서 콜레스테롤 대사 관련 유전자 발현을 측정함에 따라 조사하였다. 블랙 초크베리는 콜레스테롤 대사와 관련하여 콜레스테롤 흡수, 생합성, 유출과 관련된 유전자 발현을 조절하는 것으로 나타났다. 이는 블랙 초크베리의 혈중 콜레스테롤 저하 효과가 콜레스테롤 및 담즙 대사 관련 유전자 발현을 조절함에 의한 것으로 사료된다. 추후 블랙 초크베리 내 어떠한 생리활성물질이 콜레스테롤 대사 유전자 발현을 조절하여 이러한 효과를 나타내는 지에 대한 연구가 필요하며, 블랙 초크베리의 콜레스테롤 저하 효과를 동물 및 임상에서 기전 연구를 진행하여 천연 유래 기능성 소재로서의 블랙 초크베리의 중요성을 검증할 필요가 있을 것으로 사료된다.

Abstract ▼ AI-Helper

Black chokeberry (Aronia melanocarpa), a rich source of polyphenols, exerts hypocholesterolemic effects. However, little is known about its effects on the regulation of the hepatic cholesterol metabolism and the underlying mechanisms. In the present study, the effects of polyphenol-rich black chokeberry extract (CBE) on hepatic cholesterol metabolism were investigated by measuring the expression of genes involved in the absorption, de novo synthesis, and efflux of cholesterol in HepG2 cells. There was a significant reduction in the expression levels of genes involved in cholesterol metabolism, the low-density lipoprotein receptor, 3-hydroxy-3-methylglutaryl coenzyme A reductase, and sterol regulatory element-binding protein 2, in CBE-treated HepG2 cells. Meanwhile, CBE increased the expression levels of genes involved in cholesterol and bile acid efflux. The expression levels of mitochondrial fatty acid oxidation genes increased, whereas those of lipogenic genes decreased following CBE treatment. These data suggest that the consumption of black chokeberry may be beneficial for the prevention of hypercholesterolemia.

주제어

표/그림 (4)

그림 Fig. 1. Effects of CBE on the expressions of genes involved in cholesterol absorption and biosynthesis in HepG2 cells. Data are expressed as relative expressions to control. Bars with different letters are significantly different (p< .05). Values are means±SEM; n=6. CBE, polyphenolrich chokeberry extract; SR-B1, scavenger receptor class type I; ABCA1, ATP-binding cassette transporter A1; LDLR, low-density lipoprotein receptor; LRP1, LDLR-related protein 1; HMGR, HMG-CoA reductase; SREBP2, sterol regulatory element-binding protein 2
그림 Fig. 2. Effects of CBE on the protein levels involved in cholesterol absorption and biosynthesis in HepG2 cells. The representative images of the western blot is shown. β-actin was used as a loading control.CBE, polyphenol-rich chokeberry extract; SRB1, scavenger receptor class type I; ABCA1, ATP-binding cassette transporter A1; LDLR, low-density lipoprotein receptor; LRP1, LDLR-related protein 1; HMGR, HMG-CoA reductase; SREBP2, sterol regulatory element-binding protein 2
그림 Fig. 3. Effects of CBE on the cholesterol efflux-related genes in HepG2 cells. (A) mRNA expressions in CBE-treated HepG2 cells. Data are expressed as relative expressions to control. Bars with different letters are significantly different (p< .05). Values are means±SEM; n=6. (B) Western blot images in CBE-treated HepG2 cells. CBE, polyphenol-rich chokeberry extract; ABCG5/G8, ATP-binding cassette transporter G5/ G8; NPC1L1, Niemann-Pick C1-Like 1; CYP7A1, Cholesterol 7α-hydroxylase; ABCB11, ATP-binding cassette transporter B11; ABCB4, ATP-binding cassette transporter B4
그림 Fig. 4. Effects of CBE on the expressions of genes involved in fatty acid metabolism in HepG2 cells. (A) mRNA expression for fatty acid oxidation in CBE-treated HepG2 cells. (B) expression of lipogenic genes in CBE-treated HepG2 cells. Data are expressed as relative expressions to control. Bars with different letters are significantly different (p< .05). Values are means±SEM; n=6. CBE, polyphenol-rich chokeberry extract; CPT-1, Carnitine palmitoyltransferase 1; ACOX, acyl-coenzyme A oxidase; ACC, acetyl-coenzyme A carboxylases; FAS, fatty acid synthase; SCD-1, Stearoyl-CoA desaturase1; SREBP1c, sterol regulatory element-binding protein 1

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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