[논문]LPS로 유도된 미세아교세포에서 작약감초탕의 항염증 효과

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doi:10.6116/kjh.2022.37.5.83.

LPS로 유도된 미세아교세포에서 작약감초탕의 항염증 효과
Anti-inflammatory activity of jakyakgamcho-tang on Lipopolysaccharide-Stimulated BV-2 Microglia Cells 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.37 no.5, 2022년, pp.83 - 88

Abstract ▼ AI-Helper

Objectives : Jakyakgamcho-tang (JGT) has been traditionally used to treat muscular convulsion and pain in South Korea. According to recent studies, JGT has been reported to have anti-depression, anti-inflammation, anti-oxidative, anti-diabetics, anti-spasm and analgesic effects, but studies on its anti-neuroinflammatory and neuroprotective effect have not been deeply conducted. Thus, we investigated the anti-neuroinflammatory activity of JGT on lipopolysaccharide (LPS)-stimulated mouse microglia cells. Methods : To investigate the anti-neuroinflammatory effects of JGT on BV2 microglial cells, we examined the production of nitric oxide (NO) using griess assay, and mRNA expressions of pro-inflammatory cytokines such as interleukin (IL)-1𝛽, IL-6, and tumor necrosis factor (TNF)-𝛼 using real time RT-PCR. Furthermore, to determine the regulating mechanisms of JGT, we investigated the heme oxygenase (HO)-1 by real time RT-PCR. Results : Pre-treatment of JGT effectively decreased NO production in LPS-stimulated BV2 cells at concentrations without cytotoxicity. Additionally, JGT significantly suppressed the production of IL-1𝛽, IL-6, and TNF-𝛼 in LPS-stimulated BV2 cells. Furthermore, JGT activated the HO-1 expression, which is one of the immunomodulatory signaling molecules. And the abolishment of HO-1 by tin protoporphyrin IX (SnPP, the HO-1 inhibitor) reversed the anti- inflammatory activity of JGT in LPS-stimulated BV2 cells. Conclusions : Our results suggest that the JGT has anti-neuroinflammatory effect through the activation of HO-1 in LPS-stimulated BV2 cells. Thereby, JGT could expected to be used for the prevention and treatment of neurodegenerative disease related to neuroinflammation.

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표/그림 (5)

표 Table 1. Sequence of primers used for Real-Time PCR
그림 Fig. 1. Effect of JGT on cell viability in BV2 cells. BV2 cells were treated for 24 h with the saline or JGT as indicated concentrations. Cell viability was determined by WST assay, as described in Methods. Data are the mean ± S.D. *P <0.05 versus the saline treatment.
그림 Fig. 3. Effect of JGT on inflammatory cytokine expression in LPS-stimulated BV2 cells. BV2 cells were incubated for 1 h with saline or JGT, and then stimulated for 6 h with LPS (1 μg/mL). The mRNA expression level of IL-1β, IL-6, TNF-α were measured by qPCR. Data are the mean ± S.D. *P <0.05 versus the saline, ^†P <0.05 versus the LPS only.
그림 Fig. 2. Effect of JGT on LPS-induced nitrite production in BV2 cells. BV2 cells were incubated with saline, or JGT (0.1, 0.5, and 1 mg/mL) and then stimulated for 24 h with LPS (1 μg/mL). The concentrations of the nitrite were determined by Griess reagent as described in methods. Data are the mean ± S.D. *P <0.05 versus the saline, ^†P <0.05 versus the LPS only
그림 Fig. 4. Effect of JGT on HO-1 expression in LPS-stimulated BV2 cells. (A) BV2 cells were incubated for 6 h with saline or JGT. The mRNA expression level of HO-1 was measured by qPCR. (B) BV2 cells were pre-treated with DMSO or SnPP (5 μM), then incubated with saline, or JGT (0.1, 0.5, and 1 mg/mL) and then stimulated for 24 h with LPS (1 μg/mL). The concentrations of the nitrite were determined by Griess reagent. Data are the mean ± S.D. *P <0.05 versus the saline, ^†P <0.05 versus the DMSO.

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LPS로 유도된 미세아교세포에서 작약감초탕의 항염증 효과
Anti-inflammatory activity of jakyakgamcho-tang on Lipopolysaccharide-Stimulated BV-2 Microglia Cells 원문보기

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LPS로 유도된 미세아교세포에서 작약감초탕의 항염증 효과 Anti-inflammatory activity of jakyakgamcho-tang on Lipopolysaccharide-Stimulated BV-2 Microglia Cells 원문보기

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LPS로 유도된 미세아교세포에서 작약감초탕의 항염증 효과
Anti-inflammatory activity of jakyakgamcho-tang on Lipopolysaccharide-Stimulated BV-2 Microglia Cells 원문보기

표/그림 (5)

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