[논문]털부처꽃의 부위별 성분 분석

김희영; 박예진; 이주연; 김기영; 신수; 최민우; 홍은진; 김민정; 여수정; 박인화; 정의민; 안효진; 차윤엽

doi:10.6116/kjh.2022.37.5.89.

털부처꽃의 부위별 성분 분석
Analysis of Components in the Different Parts of Lythrum salicaria L. 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.37 no.5, 2022년, pp.89 - 96

김희영 (상지대학교 한의과대학 약리학교실) , 박예진 (상지대학교 한의과대학 약리학교실) , 이주연 (상지대학교 한의과대학 약리학교실) , 김기영 ((주)바이오포트코리아 기업부설연구소) , 신수 ((주)바이오포트코리아 기업부설연구소) , 최민우 ((주)파이힐) , 홍은진 ((주)바이오포트코리아 기업부설연구소) , 김민정 (상지대학교 한의과대학 침구의학교실) , 여수정 (상지대학교 한의과대학 경혈학교실) , 박인화 (상지대학교 한의과대학 한의학과 재활의학과) , 정의민 (상지대학교 한의과대학 내과) , 안효진 (상지대학교 한의과대학 약리학교실) , 차윤엽 (상지대학교 한의과대학 한의학과 재활의학과)

Abstract ▼ AI-Helper

Objectives : This research was performed to analyze the components in the different parts of Lythrum salicaria L. and to compare which parts of L. salicaria L. are appropriate for food development. Methods : L. salicaria L. was extracted in 20% EtOH at 100 ℃ for 4 hours. Cytotoxicity was investigated in 3T3-L1 cells after treatment of 10-500 ㎍/ml L. salicaria L. for 24 hours. Total polyphenol content (TPC) was estimated using 1 N Folin-ciocateu reagent. 2,2-Diphenyl-1-picryhydrazyl (DPPH) radical scavenging activity was estimated using DPPH reagent and gallic acid. The chemical composition was analyzed by high-performance liquid chromatography (HPLC). 1) Results : The half maximal inhibitory concentration (IC50) in the extracts of the whole plant, aerial parts, and root parts was 350 ㎍/ml, over 500 ㎍/ml, and 150 ㎍/ml, respectively. The TPC in the extracts of the whole plant, aerial parts, and root parts was 527.1 mg/g, 422.6 mg/g, and 781.1 mg/g, respectively. The averages of vitexin contents in the aerial parts, and root parts were 256.7 ± 154.9 ㎍/g and 266.1 ± 63.2 ㎍/g, respectively. The averages of TPC in the leaves, roots, flower stalks and stems were 224.0 ± 53.7 tannin acid (TA) mg/g, 221.8 ± 70.2 TA mg/g, 249.8 ± 34.4 TA mg/g, and 67.7±8.9 TA mg/g, respectively. The averages of DPPH radical scavenging activity in the leaves, roots, flower stalks, and stems were 282.01 ± 43.3 gallic acid equivalent (GAE) 𝜇mole/g, 260.16 ± 44.1 GAE 𝜇mole/g, 288.0 ± 9.3 GAE 𝜇mole/g, and 97.6 ± 10.7 GAE 𝜇mole/g, respectively. Conclusions : There were no significant differences in the content of components or antioxidant activity in the aerial parts compared to those in the whole plant of L. salicaria L. Furthermore, the root parts had low extract yield, cytotoxicity, and quality control problems, therefore our results suggest that the use of the aerial part of L. salicaria L. would be the most appropriate for food development.

주제어

표/그림 (7)

표 Table 1. Extraction of Each Part from L. salicaria L.
그림 Figure 1. The cell viability on the extract of different parts of L. salicaria L. in 3T3-L1 cells. (A) Whole plant, (B) Aerial part, (C) Root. *p < 0.05 compared with non-treated group.
표 Table 2. TPC of Extract from the Different Parts of L. salicaria L.
그림 Figure 2. HPLC analysis of vitexin standard and L. salicaria L. (A) Vitexin standard, (B) Leaf, (C) Flower stalk, (D) Stem, (E) Root.
표 Table 3. Vitexin Content of the Different Parts of L. salicaria L.
표 Table 4. TPC of the Different Parts of L. salicaria L.
표 Table 5. DPPH Radical Scavenging Activity of the Different Parts of L. salicaria L.

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