[논문]복숭아혹진딧물, Myzus persicae, 방제를 위한 제충국, 데리스, 고삼 추출물의 살충농도와 살충시간 결정

조가희; 김효정; 한송희; 김영철

doi:10.5656/ksae.2023.11.0.029

복숭아혹진딧물, Myzus persicae, 방제를 위한 제충국, 데리스, 고삼 추출물의 살충농도와 살충시간 결정
Determination of Lethal Concentrations and Lethal Times of Extracts from Tanacetum cineariaiaefolium, Derris elliptica, and Sophora flavescens, to Control Green Peach Aphid, Myzus persicae 원문보기

한국응용곤충학회지 = Korean journal of applied entomology, v.62 no.4, 2023년, pp.267 - 275

조가희 (전남대학교 농업생명과학대학 응용생물학과) , 김효정 (전라남도 농업기술원) , 한송희 (주식회사 현농) , 김영철 (전남대학교 농업생명과학대학 응용생물학과)

초록
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제충국(Tanacetum cineariaiaefolium), 데리스(Derris elliptica), 고삼(Sophora flavescens) 추출물은 다양한 해충을 방제하는데 사용되고 있다. 하지만, 국내에서 판매되고 있는 식물추춞물 자재는 유효성분의 표기가 없고, 살충농도와 살충시간에 대한 자료가 전무한 상황이다. 본 연구에서는 상용화된 주요 식물추출물의 살충유효성분의 농도를 결정하고 복숭아혹진딧물에 대해 살충농도와 살충시간을 측정하였다. 식물추출물의 살충활성성분인 pyrethrins, rotenone, matrine과 oxymatrine의 농도는 액체 크로마토그래피에서 표준물질을 활용하여 질량분석을 통해 측정하였다. 식물추출물을 농도별로 희석하여 복숭아혹진딧물에 살포하여 살충력을 측정하였다. 표준화합물과 비교한 후 질량분석 및 결정했습니다. Myzus persicae에 대한 lethal concentation과 lethal time을 조사했다. 살포 후 48시간 후 치사 농도(LC50)는 pyrethrins (20.4 ppm), roteone (34.1 ppm), matrine (29.6 ppm)였고, 100 ppm 살포한 LT50은 pyrethrins (13.4시간), rotenone (15.1시간), matrine (14.4시간)로 측정되었다. Kaplan-Meier 생존분석 결과, 100 ppm에서 세 가지 식물 추출물의 LT50은 대조구인 화학 살충제인 Sulfoxaflor를 살포 처리구보다 유의하게 빨랐습니다. 본 결과는 복숭아혹진딧물 방제를 위해 식물추출물의 제형화에 단일 또는 혼합 제제를 개발하는데 기준 살충농도와 살충시간을 제고하는데 의미가 있다.

Abstract ▼ AI-Helper

Botanical extracts are employed in management of aphids. Extracts from Tanacetum cineariaiaefolium, Derris elliptica, and Sophora flavescens are widely used to control various insects. In this study, we determined concentrations of insecticidal active ingredients in commercial botanical extracts of these plants, and we investigated the time and concentration for lethal results with the green peach aphid, Myzus persicae. The concentrations of active ingredients, pyrethrins from T. cineariaiaefolium, rotenone from D. elliptica, and matrine and oxymatrine from S. flavescens, were determined after their fractionation by liquid chromatography followed by mass analysis and comparison with standard compounds. The extracts were tested for lethality in a bioassay with green peach aphids. Sprays at defined doses were applied to tobacco leaves infested with aphid nymphs. The lethal concentrations (LC50) were 20.4 ppm for pyrethrins, 34.1 ppm for rotenone, and 29.6 ppm for matrine at 48 h after treatments. At 100 ppm application levels, the lethal time LT50 was 13.4 h for pyrethrin, 15.1 h for rotenone, and 14.4 h for matrine. Kaplan-Meier analysis indicated the lethal times for the three botanical extracts at 100 ppm were significantly faster than application of a chemical insecticide, Sulfoxaflor, applied at the recommended level. These results provide baselines to develop and formulate single or mixed preparations containing botanical extracts to control green peach aphids on commercial crops.

주제어

표/그림 (7)

표 Table 1. Quantification of major active insecticide ingredients in the commercial liquid botanical extracts
그림 Fig. 1. Corrected mortality of green peach aphids by time period after treatment of defined concentrations in the pyrethrins of T. cineariaiaefolium commercial liquid extract and Sulfoxaflor. Data are the means and standard errors from three independent experiments each with three replicates of the treatments for total pyrethrins in the botanical extract. One-way analysis of variance (ANOVA; P < 0.05 was considered significant) was performed using IBM SPSS software version 23 (IBM Corp., Armonk, NY, USA); Duncan's multiple range test (P < 0.05) further compared differences if the F test was significant. The lowercase letters indicate statistical differences. Abbott's corrected mortality (%) = (mortality of treatment-mortality of control) / (100-mortality of control) x 100 was used in the pyrethrin treatment.
그림 Fig. 2. Corrected mortality of green peach aphids by time period after treatment rotenone from commercial liquid extract of D. elliptica and Sulfoxaflor. Data represent three independent experiments means and standard error with three replicates each. One-way analysis of variance (ANOVA; P < 0.05 was considered significant) analyzed data using IBM SPSS software version 23 (IBM Corp., Armonk, NY, USA); Duncan's multiple range test (P < 0.05) further compared differences if the F test was significant, and lowercase letters indicate statistical differences. Abbott's corrected mortality (%) = (mortality of treatment-mortality of control) / (100-mortality of control) x 100 was used in the pyrethrin treatment.
그림 Fig. 3. Corrected mortality of green peach aphids by time period after treatment by matrine contained in the commercial S. favescens liquid extract and Sulfoxaflor. Data represent three independent experiments means and standard error with three replicates each. One-way analysis of variance (ANOVA; P < 0.05 was considered significant) analyzed data using IBM SPSS software version 23 (IBM Corp., Armonk, NY, USA); Duncan's multiple range test (P < 0.05) further compared differences if the F test was significant, and lowercase letters indicate statistical differences. Abbott's corrected mortality (%) = (mortality of treatment-mortality of control) / (100-mortality of control) x 100 was used in the pyrethrin treatment.
표 Table 2. Lethal concentration (mg/L) of based on pyrethrins, rotenone, and matrine in commercial botanical extracts against Myzus persicae
표 Table 3. Lethal time (hours) of the efficacy of pyrethrins, rotenone, matrine in commercial botanical extracts against Myzus persicae
그림 Fig. 4. Kaplan-Meier survival plots of green peach aphids (n = 90) treated with 100 ppm (A) and 50 ppm (B) of pyrethrins, rotenone, matrine from the commercial plant liquid extracts or for both (A) and (B) Sulfoxaflor, 25 ppm. Green peach aphid mortalities were monitored at defined hours post-treatment. Data points represent the average of three experiments with three replicates each. One representative data set is shown. P values indicate significant differences between two concentrations in multiple comparisons as determined by the Log-Rank test.

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