[논문]사상자의 세포자기살해능이 자궁경부암세포에 미치는 영향

안상현; 김기봉; 권나연; 박장경

doi:10.15204/jkobgy.2023.36.1.023

사상자의 세포자기살해능이 자궁경부암세포에 미치는 영향
Effects of Apoptosis of Torilis Japonica on Cervical Cancer Cell 원문보기

大韓韓方婦人科學會誌 = The Journal of Korean obstetrics & gynecology, v.36 no.1, 2023년, pp.23 - 34

안상현 (세명대학교 한의과대학 해부학교실) , 김기봉 (부산대학교 한의학전문대학원 한방소아과) , 권나연 (가천대학교 한의과대학 부인과교실) , 박장경 (부산대학교 한의학전문대학원 한방부인과)

Abstract ▼ AI-Helper

Objectives: This study was conducted to confirm the anticancer effect of Torilis Japonica (TJ) on cervical cancer and to determine whether the effect was apoptosis. Methods: In this study, the effect of TJ extract on toxicity, mitochondrial morphology, nuclear morphological changes, Extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) pathway were investigated in HeLa cell, human cervical cancer cell. Results: The cytotoxicity, ratio of cells with nuclear changes to the total number of cells, and P38 phosphorylation increased in a concentration-dependent manner after administration of TJ extract. The length of mitochondria and ERK phosphorylation in HeLa cells decreased in a concentration-dependent manner after administration of TJ extract. Conclusions: TJ extract has an anticancer effect on cervical cancer cells, which is presumed to be due to apoptosis, and showed potential as a future cervical cancer treatment.

주제어

표/그림 (5)

그림 Fig. 1. The cell viability of HeLa cells at various TJ extract concentrations. HeLa cells were incubated with the different concentration of TJ extract (0.125, 0.25, 0.5, 0.75 and 1.0 mg/ml) for 24 hours. (A) The HeLa cell morphology were observed using the microscope. (B) To analyze the cell viability, the cells were incubated with WST-1 solution for 2 hours. Data are expressed as 100% for the untreated group. Data represents the mean±standard deviations (n=3). * : p<0.05, statistically significant difference compared with untreated group.
그림 Fig. 2. TJ extract induces cell death in HeLa cells. HeLa cells were treated with TJ extract (0.75 and 1.0 mg/ml) for 24 hours, respectively. (A) The cells were treated with Live/Dead cell probe for 30 minutes. And then, cell death was observed using the fluorescence microscopy. (B) The bar graphs indicated the dead cells per live cells ratio. The percentage of the dead cell in the untreated group was give 100. Data represents the mean±standard deviations (n=3). * : p<0.05, statistically significant difference compared with untreated group.
그림 Fig. 3. Analysis of mitochondrial morphology on in TJ extract-treated HeLa cells. The HeLa cells were treated with TJ extract (0.75 and 1.0 mg/ml) for 24 hours and the cells were incubated with Mito-Tracker for 30 minutes at 37℃. (A) The photographs show the mitochondrial morphology. (B) The bar graph was obtained from panel B. The percentage of the mitochondrial length in the untreated group was given 100. Data are expressed as 100% for the untreated group. Data represents the mean±standard deviations (n=3). * : p<0.05, statistically significant difference compared with untreated group.
그림 Fig. 4. Analysis of nuclear morphological changes in TJ extract-treated HeLa cells. The cells were treated with TJ extract (0.75 and 1.0 mg/ml) for 24 hours and the nucleus was stained with Hoechst. (A) The photographs represent the Hoechst-positive cells. (B) The bar graph was obtained from panel B. The percentage of the Hoechst-positive cell in the untreated group was give 100%. Data represents the mean±standard deviations (n=3). * : p<0.05, statistically significant difference compared with untreated group.
그림 Fig. 5. Effect of TJ extract on MAPK kinase activity in HeLa cells. (A and B) HeLa cells were treated with TJ extract (0.75 and 1.0 mg/ml) for 24 hours. The expression of ERK and p38 were determined using the specific antibodies such as anti-phosphor ERK and phosphor p38. The relative expression of phosphorylated JNK, relative to the untreated group. Data represents the mean±standard deviations (n=6). *: p<0.05, statistically significant difference compared with untreated group.

참고문헌 (35)

Korean Medicine Obstetrics & Gynecology？compilation committee. Korean Medicine？Obstetrics & Gynecology (Vol 2). 4th ed.？Seoul:Jeongdam Publisher. 2021:393, 401.
Korean Society of Obstetrics and Gynecology.？Gynecology. 6th ed. Paju:Koonja Publisher.？2021:771.
National cancer information center. Cancer？incidence rate by age group. [Cited January？8,2022]. Available from:https://cancer.go.kr/lay1/S1T639C642/contents.do
Ministry of Health and Welfare. 2020？National Cancer Registry Statistics？Reference. 2022.
Lee JW. ASTI MARKET INSIGHT？82: Cervical Cancer Treatment. ASTI？MARKET INSIGHT;2022-082.
Jo HJ, et al. Effects of Scutellariae？Radix on Gene Expression of Human？Cervical Cancer Cells(SNU-703). J？Korean Obstet Gynecol. 2009;22(3):117-34.
Kim BH, Baek SH. The Effects of？the Stem of Spatholobus suberectus？Dunn on the Proliferation and Gene？Expression Related Apoptosis in Human？Cervical Cancer Cells. J Korean Obstet？Gynecol. 2005;18(1):169-80.
Lee JG, et al. Growth Arrest and Apoptosis？of Human Uterine Cervical Carcinoma？Cells Induced by Trichosanthes Semen？Extract. Korean J Oriental Physiology？& Pathology. 2005;19(4):965-72.
Lee HH, Lim EM. Growth Inhibition？and Apoptosis Iudction of Trichosanthis？Radix Extract on Human. J Korean？Obstet Gynecol. 2005;18(3):77-91.
Huh J. Donguibogam(東醫寶鑑). 1st.？Seoul:Bubin Publishers. 2007:2165.
Kim GS, et al. Anti-biofilm Effects？of Torilis Japonica Ethanol Extracts？against Staphylococcus Aureus. J？Microbiol Biotechnol. 2022;32(2):220-7.

원문보기 상세보기
Lee KY, Song EH, Hong CH. Effects？of Torilis Japonica Extract on DNCB-induced Contact Dermatitis in BALB/cMice. J Korean Med Ophthalmol？Otolaryngol Dermatol. 2016;29(4):61-77.

원문보기 상세보기
Jung HW, Ghil SH. A Torilis Japonica？Extract Exerts Anti-proliferative Activities？on the U87MG Human Glioblastoma？Cell Line. Mol Med Rep. 2010;3(6):1041-5.

상세보기
Kim GT, Kim SY, Kim YM. Torilis？Japonica Extract Fraction Compound,？EGFR-targeted Inhibition of Cancer？Abnormal Metastasis in A549 Lung？Cancer Cells. Oncol Rep. 2017;38(2):1206-12.

상세보기
Song DH, et al. Sesquiterpenes from？Fruits of Torilis Japonica with Inhibitory？Activity on Melanin Synthesis in B16？cells. J Nat Med. 2018;72(1):155-60.

상세보기
Nationwide College of Oriental Medicine.？Herbalism edited by Professor. Herbalism.？1st. Seoul:Younglim Inc. 2006:628-9.
Martinez-Reyes I, Chandel NS. Cancer？Metabolism: Looking forward. Nat Rev？Cancer. 2021;21(10):669-80.
Park JG, Pyun WY, Park HW. Cancer？Metabolism: Phenotype, Signaling and？Therapeutic targets. Cells. 2020;9(10):2308.

상세보기
Hanahan D, Weinberg RA. The Hallmarks？of Cancer. Cell. 2000;100(1):57-70.

상세보기
Pistritto G, et al. Apoptosis as Anticancer？Mechanism: Function and Dysfunction？of Its Modulators and Targeted？Therapeutic Strategies. Aging(Albany？NY). 2016;8(4):603-19.

상세보기
Wong RSY. Apoptosis in Cancer: from？Pathogenesis to Treatment. J Exp Clin？Cancer Res. 2011;30(1):87.

상세보기
Cho JG. Oriental clinical oncology.？Daejeon:Ju Min Publishers. 2001:799-818.
Hwang EI, et al. Isolation and Development？of Quantitative Determination of Torilin？from the Torilis Fructus. Kor J？Pharmacogn. 2007;38(4):321-6.
Lee EB, et al. Isolation of torilin form？Torilis Japonica Fruit and Its Analgesic？and Anti-inflammatory Activities. Kor？J Pharmacogn. 1999;30(2):137-44.
Kim MS, et al. Anti-invasive Activity？of Torilin, a Sesquiterpene Compound？Isolated from Torilis Japonica. Oncol？Rep. 2001;8(2):359-64.
Kim MS, et al. Anti-angiogenic Activity？of Torilin, a Sesquiterpene Compound？Isolated from Torilis Japonica. Int J？Cancer. 2000;87(2):269-75.

상세보기
Park HW, et al. Guaiane sesquiterpenoids？from Torilis japonica and their cytotoxic？effects on human cancer cell lines.？Arch Pharm Res. 2006;29(2):131-4.

원문보기 상세보기
Kim DH, et al. Antitumor mechanism？of combination of Angelica gigas and？Torilis japonica in LNCaP prostate？cancer cells via G1 arrest and inhibition？of Wnt/β-catenin and androgen receptor？signaling. Phytother Res. 2022;36(7):2999-3008.
Lee SJ, et al. Herbal Medicine for Cervical？Intraepithelial Neoplasia after Loop？Electrosurgical Excision Procedure: A？Systematic Review and Meta-Anaylsis.？J Korean Obstet Gynecol. 2020;33(2):44-62.

원문보기 상세보기
Park KD, Bae JE, Yoon YJ. Systematic？Review of Fumigation Therapy for？Atorphic Verginitis. J Korean Obstet？Gynecol. 2016;29(1):92-101.
Hwang YS, et al. Analysis for Randomized？Controlled Clinical Trials of Korean？Medicine External Treatment on？Bacterial Vaginosis. J Korean Obstet？Gynecol. 2019;32(2):50-70.

원문보기 상세보기
Bulavin DV, Fornace AJ. P38 MAP？Kinase's Emerging Role as a Tumor？Suppressor. Advances in cancer research.？2004;92:95-118.

상세보기
Guo YJ, et al. ERK/MAPK Signalling？Pathway and Tumorigenesis. Exp Ther？Med. 2020;19(3):1997-2007.

상세보기
Xia Z, et al. Opposing Effects of ERK？and JNK-p38 MAP Kinases on Apoptosis.？Science. 1995;270(5240):1326-31.

상세보기
Eidet JR, et al. Objective Assessment？of Changes in Nuclear Morphology and？Cell Distribution Following Induction？of Apoptosis. Diagn Pathol. 2014;9:92.

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증