[논문]효소처리한 미선나무 잎의 용매 추출 후 이화학적 품질 변화

이경행; 장다빈; 이재준; 한기정; 배경아; 이원종; 권순영; 이호진

doi:10.9799/ksfan.2023.36.1.042

효소처리한 미선나무 잎의 용매 추출 후 이화학적 품질 변화
Changes in Physicochemical Quality of the Extracts by Solvents in the Enzyme-Treated Abeliophyllum distichum Leaves 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.36 no.1, 2023년, pp.42 - 49

이경행 (한국교통대학교 식품영양학 전공) , 장다빈 (한국교통대학교 식품영양학 전공) , 이재준 (한국교통대학교 식품영양학 전공) , 한기정 (한국교통대학교 식품영양학 전공) , 배경아 (한국교통대학교 식품영양학 전공) , 이원종 (한국교통대학교 식품영양학 전공) , 권순영 (미선나무식품화사업단) , 이호진 (한국교통대학교 식품영양학 전공)

Abstract ▼ AI-Helper

To enhance the efficacy of Abeliophyllum distichum leaves, extracts were prepared using different solvents for hydrolytic enzyme-treated Abeliophyllum distichum leaves. Physicochemical quality and antioxidant activity were measured. Soluble solids, reducing sugar, ascorbic acid, flavonoids, and polyphenols contents showed the lowest values in the control without enzyme treatment. However, they showed high contents in ethanol extract. In the case of enzyme treatment, their values were higher than those of the control. In particular, verbascoside content increased about 220 times more than that of the control group when treated with enzymes and extracted with 50% ethanol. pH was lowered upon enzymatic treatment. Regarding DPPH radical scavenging activity, for enzyme-free, 25% ethanol extract showed the highest activity among extracts with different solvents. For cellulase and pectinase-treated leaves, water extract showed the highest DPPH radical scavenging activity among extracts with different solvents. For leaves treated with enzyme combination, 50% ethanol extract showed the highest DPPH radical scavenging activity among extracts with different solvents. Regarding ABTS radical scavenging activity, it was generally higher in the 50% ethanol extract than in the water extract and 25% ethanol extract. In particular, verbascoside content was increased when the extract was prepared by co-treatment with enzymes and 50% ethanol.

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표/그림 (5)

표 Table 1. HPLC gradient conditions for the analysis of verbascoside in the enzyme-treated Abeliophyllum distichum leaves extracts
표 Table 2. Changes in soluble solid, reducing sugar content and pH in the enzyme-treated Abeliophyllum distichum leaves extracts
표 Table 3. Changes in verbascoside content in the enzyme-treated Abeliophyllum distichum leaves extracts (unit: μg/mL)
표 Table 4. Changes in ascorbic acid, flavonoids and polyphenols contents in the enzyme-treated Abeliophyllum distichum leaves extracts (unit: mg%)
표 Table 5. Changes in in vitro antioxidant activities in the enzyme-treated Abeliophyllum distichum leaves extracts (unit: %)

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효소처리한 미선나무 잎의 용매 추출 후 이화학적 품질 변화
Changes in Physicochemical Quality of the Extracts by Solvents in the Enzyme-Treated Abeliophyllum distichum Leaves 원문보기

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효소처리한 미선나무 잎의 용매 추출 후 이화학적 품질 변화 Changes in Physicochemical Quality of the Extracts by Solvents in the Enzyme-Treated Abeliophyllum distichum Leaves 원문보기

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효소처리한 미선나무 잎의 용매 추출 후 이화학적 품질 변화
Changes in Physicochemical Quality of the Extracts by Solvents in the Enzyme-Treated Abeliophyllum distichum Leaves 원문보기

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