[논문]생쥐 대장 카할세포의 자발적 탈분극에서 한약의 효과에 관한 비교연구

최나리; 정해정; 최우균; 김병주

doi:10.14374/hfs.2023.31.1.11

[국내논문] 생쥐 대장 카할세포의 자발적 탈분극에서 한약의 효과에 관한 비교연구
Effects of Herbal medicines in Pacemaker Potential of Colonic Intestinal Interstitial cells of Cajal in mice 원문보기

大韓韓醫學方劑學會誌 = Herbal formula science, v.31 no.1, 2023년, pp.11 - 19

최나리 (부산대학교 한의학전문대학원 양생기능의학교실) , 정해정 (부산대학교 한의학전문대학원 양생기능의학교실) , 최우균 (부산대학교 한의학전문대학원 양생기능의학교실) , 김병주 (부산대학교 한의학전문대학원 양생기능의학교실)

Abstract ▼ AI-Helper

Objectives : The purpose of this study was to examine the effects of herbal medicines on pacemaker potentials of large intestinal interstitial Cells of Cajal (ICC) in mice. Methods : We made the ICC culture in large intestine in mice and used the electrophysiological method to record pacemaker potentials. Also we used MTT assay to check cell viability and examined the ICC protein expression by western blot. Results : 1.Glycyrrhiza uralensis Fischer (GF) (50-150 ㎍/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 58.95 ㎍/ml. Angelica gigas (AG) (50-200 ㎍/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 77.22 ㎍/ml. Poncirus fructus (PF) (10-100 ㎍/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 13.39 ㎍/ml. Citrus unshiu S. Marcov. (CU) (10-500 ㎍/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 139.80 ㎍/ml. Gardenia jasminoides J. Ellis (GJ) (100-500 ㎍/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 78.70 ㎍/ml. Coptis chinensis (CC) (100-1000 ㎍/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 138.10 ㎍/ml. Scutellaria baicalensis (SB) (10-100 ㎍/ml) had no effects on pacemaker potentials and decreased frequency with concentration-dependent manners. IC50 is 18.34 ㎍/ml. Atractylodes macrocephala koidzumi (AM) (10-100 ㎍/ml) induced pacemaker hyperpolarizations and decreased frequency with concentration-dependent manners. IC50 is 18.54 ㎍/ml. 2. PF, SB and AM had no effects on cell death in large ICC. 3. PF increased the ANO1 and c-kit protein expression and SB and AM increased the c-kit protein expression in large ICC. Conclusions : These results suggest that PF, SB, and AM are likely to be the optimal combination of herbal medicines that can be used to treat diseases such as gastrointestinal motility disorders such as irritable bowel syndrome.

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표/그림 (4)

그림 Fig. 1. Effects of GF, AG, PF, and CU herbal medicines in pacemaker potentials of murine colonic intestinal ICC. (A) a) GF depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. (B) a) AG depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. (C) a) PF depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. (D) a) CU depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. GF, Glycyrrhiza uralensis Fischer, AG, Angelica gigas, PF, Poncirus fructus, CU, Citrus unshiu S. Marcov..
그림 Fig. 2. Effects of GJ, CC, SB, and AM herbal medicines in pacemaker potentials of murine colonic intestinal ICC. (A) a) GJ depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. (B) a) CC depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. (C) a) SB depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. (D) a) AM depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. GJ, Gardenia jasminoides J. Ellis, CC, Coptis chinensis, SB, Scutellaria baicalensis, AM, Atractylodes macrocephala koidzumi.
그림 Fig. 3. PF, SB or AM has no effect on murine colonic ICC viability after 24 h. (A) PF, (B) SB or (C) AM has no effect on murine colonic ICC viability. Results are presented as the mean ± SEM. PF, Poncirus fructus. SB, Scutellaria baicalensis. AM, Atractylodes macrocephala koidzumi.
그림 Fig. 4. Effects of PF, SB and AM on the expression of ANO1, c-kit and TRPM7 in murine colonic ICC. (A) The expression of ANO1 and c-kit increased considerably but that of TRPM7 was almost unchanged by PF. (B) The expression of c-kit increased considerably but that of ANO1 and TRPM7 was almost unchanged by SB. (C) The expression of c-kit increased considerably but that of ANO1 and TRPM7 was almost unchanged by AM. Results represent the mean ± SEM. ^*P<0.05. ^**P<0.01. CTRL, control; ANO1, Anoctamin1; TRPM7, transient receptor potential melastatin 7. PF, Poncirus fructus. SB, Scutellaria baicalensis. AM, Atractylodes macrocephala koidzumi.

참고문헌 (26)

Pan R, Wang L, Xu X, Chen Y, Wang H, Wang？G et al. Crosstalk between the Gut Microbiome and Colonic Motility in Chronic Constipation: Potential？Mechanisms and Microbiota Modulation. Nutrients.？2022;14:3704.

상세보기
Acharekar MV, Guerrero Saldivia SE, Unnikrishnan S,？Chavarria YY, Akindele AO, Jalkh AP et al. A？Systematic Review on the Efficacy and Safety of？Selective Serotonin Reuptake Inhibitors in Gastrointestinal？Motility Disorders: More Control, Less Risk. Cureus.？2022;14:e27691.
Zheng Z, Tang J, Hu Y, Zhang W. Role of gut？microbiota-derived signals in the regulation of？gastrointestinal motility. Front Med (Lausanne).？2022; 9:961703.

상세보기
Hwang SJ, Drumm BT, Kim MK, Lyu JH, Baker？S, Sanders KM et al. Calcium transients in intramuscular？interstitial cells of Cajal of the murine gastric？fundus and their regulation by neuroeffector transmission.？J Physiol. 2022;600:4439-63.

상세보기
Koh SD, Drumm BT, Lu H, Kim HJ, Ryoo SB,？Kim HU et al. Propulsive colonic contractions are mediated by inhibition-driven poststimulus responses？that originate in interstitial cells of Cajal. Proc？Natl Acad Sci USA. 2022;119:e2123020119.

상세보기
Walker RL, Koh SD, Sergeant GP, Sanders KM,？Horowitz B. TRPC4 currents have properties similar？to the pacemaker current in interstitial cells of？Cajal. Am J Physiol Cell Physiol. 2002;283:C1637-45.

상세보기
Kim BJ, Lim HH, Yang DK, Jun JY, Chang IY,？Park CS et al. Melastatin-type transient receptor？potential channel 7 is required for intestinal？pacemaking activity. Gastroenterology. 2005;129:1504-17.

상세보기
Zhu MH, Kim TW, Ro S, Yan W, Ward SM,？Koh SD et al. A Ca(2+)-activated Cl(-) conductance？in interstitial cells of Cajal linked to slow wave currents and pacemaker activity. J Physiol. 2009;？587:4905-18.

상세보기
Sanders KM, Koh SD, Ward SM. Interstitial cells？of cajal as pacemakers in the gastrointestinal tract.？Annu Rev Physiol. 2006;68:307-43.

상세보기
Kim D, Kim JN, Nam JH, Lee JR, Kim SC,？Kim BJ. Modulation of pacemaker potentials in？murine small intestinal interstitial cells of Cajal？by gamisoyo-san, a traditional Chinese herbal medicine. Digestion. 2018;98:56-68.

상세보기
Shim JH, Lee SJ, Gim H, Kim HJ, Han T, Kim？JG et al., Regulation of the pacemaker activities？in cultured interstitial cells of Cajal by Citrus？unshiu peel extracts. Mol Med Rep. 2016;14:3908-16.

상세보기
Sung SK, Kim SJ, Ahn TS, Hong NR, Park HS,？Kwon YK et al. Effects of Dangkwisoosan, a traditional herbal medicine for treating pain and？blood stagnation, on the pacemaker activities of？cultured interstitial cells of Cajal. Mol Med Rep.？2015;12:6370-6.

상세보기
Kim HJ, Han T, Kim YT, So I, Kim BJ.？Magnolia officinalis bark extract induces depolarization？of pacemaker potentials through M2 and M3？muscarinic receptors in cultured murine small？intestine interstitial cells of cajal. Cell Physiol？Biochem. 2017;43:1790-802.

상세보기
Lee S, Gim H, Shim JH, Kim HJ, Lee JR, Kim？SC et al., The traditional herbal medicine,？Ge-Gen-Tang, inhibits pacemaker potentials by？nitric oxide/cGMP dependent ATP-sensitive K+？channels in cultured interstitial cells of Cajal？from mouse small intestine. J Ethnopharmacol.？2015;170:201-9.

상세보기
Sanders KM, Ordog T, Koh SD, Torihashi S,？Ward SM. Development and plasticity of interstitial？cells of Cajal. Neurogastroenterol Motil. 1999;11:311-38.

상세보기
Eshraghian A, Eshraghian H. Interstitial cells of？Cajal: a novel hypothesis for the pathophysiology of？irritable bowel syndrome. Can J Gastroenterol.？2011; 25:277-9.

상세보기
Yang B, Zhou XC, Lan C. Impact of the？alterations in the interstitial cells of Cajal on？intestinal motility in post-infection irritable bowel？syndrome. Mol Med Rep. 2015;11:2735-40.

상세보기
Gomez-Pinilla PJ, Gibbons SJ, Bardsley MR, Lorincz？A, Pozo MJ, Pasricha PJ et al. Ano1 is a selective marker of interstitial cells of Cajal in？the human and mouse gastrointestinal tract. Am？J Physiol Gastrointest Liver Physiol. 2009;296:G1370-81.
Kim BJ, Park KJ, Kim HW, Choi S, Jun JY,？Chang IY et al. Identification of TRPM7？channels in human intestinal interstitial cells of？Cajal. World J Gastroenterol. 2009;15:5799-804.

상세보기
Ding F, Guo R, Cui ZY, Hu H, Zhao G. Clinical application and research progress of？extracellular slow wave recording in the gastrointestinal？tract. World J Gastrointest Surg. 2022;14:544-55.
Sanders KM. Spontaneous Electrical Activity and？Rhythmicity in Gastrointestinal Smooth Muscles.？Adv Exp Med Biol. 2019;1124:3-46.
Sanders KM, Kito Y, Hwang SJ, Ward SM.？Regulation of Gastrointestinal Smooth Muscle？Function by Interstitial Cells. Physiology (Bethesda).？2016;31:316-26.

상세보기
Babos CI, Leucuta DC, Dumitrascu DL. Meditation？and Irritable Bowel Syndrome, a Systematic Review？and Meta-Analysis. J Clin Med. 2022;11:6516.

상세보기
Jiang W, Wu J, Zhu S, Xin L, Yu C, Shen Z.？The Role of Short Chain Fatty Acids in Irritable？Bowel Syndrome. J Neurogastroenterol Motil. 2022;28:540-8.
Jang DE, Bae JH, Chang YJ, Lee YH, Nam KT,？Kim IY. Neuronal Nitric Oxide Synthase Is a Novel Biomarker for the Interstitial Cells of？Cajal in Stress-Induced Diarrhea-Dominant Irritable？Bowel Syndrome. Dig Dis Sci. 2018;63:619-27.

상세보기
Chen B, Zhu S, Du L, He H, Kim JJ, Dai N.？Reduced interstitial cells of Cajal and increased？intraepithelial lymphocytes are associated with？development of small intestinal bacterial overgrowth？in post-infectious IBS mouse model. Scand J？Gastroenterol. 2017;52:1065-71.

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