[논문]생쥐 대장 카할세포의 자발적 탈분극에서 한약의 효과에 관한 비교연구

최나리; 정해정; 최우균; 김병주

doi:10.14374/hfs.2023.31.1.11

[국내논문] 생쥐 대장 카할세포의 자발적 탈분극에서 한약의 효과에 관한 비교연구
Effects of Herbal medicines in Pacemaker Potential of Colonic Intestinal Interstitial cells of Cajal in mice 원문보기

大韓韓醫學方劑學會誌 = Herbal formula science, v.31 no.1, 2023년, pp.11 - 19

최나리 (부산대학교 한의학전문대학원 양생기능의학교실) , 정해정 (부산대학교 한의학전문대학원 양생기능의학교실) , 최우균 (부산대학교 한의학전문대학원 양생기능의학교실) , 김병주 (부산대학교 한의학전문대학원 양생기능의학교실)

Abstract ▼ AI-Helper

Objectives : The purpose of this study was to examine the effects of herbal medicines on pacemaker potentials of large intestinal interstitial Cells of Cajal (ICC) in mice. Methods : We made the ICC culture in large intestine in mice and used the electrophysiological method to record pacemaker potentials. Also we used MTT assay to check cell viability and examined the ICC protein expression by western blot. Results : 1.Glycyrrhiza uralensis Fischer (GF) (50-150 ㎍/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 58.95 ㎍/ml. Angelica gigas (AG) (50-200 ㎍/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 77.22 ㎍/ml. Poncirus fructus (PF) (10-100 ㎍/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 13.39 ㎍/ml. Citrus unshiu S. Marcov. (CU) (10-500 ㎍/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 139.80 ㎍/ml. Gardenia jasminoides J. Ellis (GJ) (100-500 ㎍/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 78.70 ㎍/ml. Coptis chinensis (CC) (100-1000 ㎍/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 138.10 ㎍/ml. Scutellaria baicalensis (SB) (10-100 ㎍/ml) had no effects on pacemaker potentials and decreased frequency with concentration-dependent manners. IC50 is 18.34 ㎍/ml. Atractylodes macrocephala koidzumi (AM) (10-100 ㎍/ml) induced pacemaker hyperpolarizations and decreased frequency with concentration-dependent manners. IC50 is 18.54 ㎍/ml. 2. PF, SB and AM had no effects on cell death in large ICC. 3. PF increased the ANO1 and c-kit protein expression and SB and AM increased the c-kit protein expression in large ICC. Conclusions : These results suggest that PF, SB, and AM are likely to be the optimal combination of herbal medicines that can be used to treat diseases such as gastrointestinal motility disorders such as irritable bowel syndrome.

주제어

표/그림 (4)

그림 Fig. 1. Effects of GF, AG, PF, and CU herbal medicines in pacemaker potentials of murine colonic intestinal ICC. (A) a) GF depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. (B) a) AG depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. (C) a) PF depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. (D) a) CU depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. GF, Glycyrrhiza uralensis Fischer, AG, Angelica gigas, PF, Poncirus fructus, CU, Citrus unshiu S. Marcov..
그림 Fig. 2. Effects of GJ, CC, SB, and AM herbal medicines in pacemaker potentials of murine colonic intestinal ICC. (A) a) GJ depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. (B) a) CC depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. (C) a) SB depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. (D) a) AM depolarized the membrane potential and decreased the frequency in pacemaker potential in colonic intestinal ICC. b) Summary of changes. GJ, Gardenia jasminoides J. Ellis, CC, Coptis chinensis, SB, Scutellaria baicalensis, AM, Atractylodes macrocephala koidzumi.
그림 Fig. 3. PF, SB or AM has no effect on murine colonic ICC viability after 24 h. (A) PF, (B) SB or (C) AM has no effect on murine colonic ICC viability. Results are presented as the mean ± SEM. PF, Poncirus fructus. SB, Scutellaria baicalensis. AM, Atractylodes macrocephala koidzumi.
그림 Fig. 4. Effects of PF, SB and AM on the expression of ANO1, c-kit and TRPM7 in murine colonic ICC. (A) The expression of ANO1 and c-kit increased considerably but that of TRPM7 was almost unchanged by PF. (B) The expression of c-kit increased considerably but that of ANO1 and TRPM7 was almost unchanged by SB. (C) The expression of c-kit increased considerably but that of ANO1 and TRPM7 was almost unchanged by AM. Results represent the mean ± SEM. ^*P<0.05. ^**P<0.01. CTRL, control; ANO1, Anoctamin1; TRPM7, transient receptor potential melastatin 7. PF, Poncirus fructus. SB, Scutellaria baicalensis. AM, Atractylodes macrocephala koidzumi.

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