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표고 자실체 물 추출물과 베타글루칸이 한우 근육위성세포 증식에 미치는 영향
Effect of Lentinula edodes water extracts and Lentinan on proliferation of myosatellite cell of Bos taurus Hanwoo 원문보기

Journal of applied biological chemistry, v.66, 2023년, pp.60 - 66  

김소희 (Department of Forest Science, Chungbuk National University) ,  오세혁 (Department of Animal Science, Chungbuk National University) ,  박상훈 (Department of Animal Science, Chungbuk National University) ,  김은진 (Department of Forest Therapy, Chungbuk National University) ,  최정석 (Department of Animal Science, Chungbuk National University) ,  이화용 (Department of Forest Science, Chungbuk National University)

초록
AI-Helper 아이콘AI-Helper

표고는 전세계적으로 많이 생산되는 버섯 중 하나이다. 베타글루칸근아세포의 증식과 분화를 증가시키며, 골격근의 기능을 향상시키는 것으로 알려져 있다. 본 연구에서는 표고 물 추출물과 표고의 베타글루칸인 렌티난이 한우 근육세포의 증식에 미치는 영향을 알아보고자 하였다. 표고 물 추출물의 베타글루칸의 함량은 85 ℃ 4시간 추출조건에서 약 15.20%, 100 ℃ 4시간 추출조건에서 약 13.64%, 40 ℃ 8시간 추출조건와 상온 24시간 추출조건에서 각각 약 9.48%와 약 8.21%였다. 표고 물 추출물을 한우 근육위성세포의 배양에 첨가하였을 때, MyoD 유전자는 40 ℃ 8시간, 그리고 100 ℃ 4시간 추출물에서 그리고 Myogenin 유전자는 40 ℃ 8시간 추출물에서 발현이 증가하였고, 증식과 활성은 무처리에 비하여 증가하지 않았다. 하지만, 근육위성세포의 배양에 렌티난의 첨가는 한우 근육위성세포의 세포분화와 근육형성에 관련된 Myogenin 유전자의 발현, 세포의 증식과 활성을 증가시켰다. 본 연구는 표고의 성분이 한우근육위성세포의 증식에 영향을 줄 수 있는 것을 확인하였으며, 추가연구를 통하여 차후 버섯 산업 및 배양육 산업의 발전에 도움을 줄 수 있을 것이다.

Abstract AI-Helper 아이콘AI-Helper

Lentinula edodes is one of the most produced mushrooms in the world. In this study, the effects of L. edodes water extracts and lentinan, a beta-glucan from this mushroom, on the proliferation of Bos taurus Hanwoo myosatellite cells were studied. The betaglucan content of the L. edodes water extract...

주제어

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