[논문]등온적정열량계를 이용한 BaCl2와 EDTA 킬레이션 결합 반응의 pH 영향

육가은; 장지웅

doi:10.14478/ace.2023.1043

등온적정열량계를 이용한 BaCl2와 EDTA 킬레이션 결합 반응의 pH 영향
Influence of pH on Chelation of BaCl2 and EDTA Using Isothermal Titration Calorimetry 원문보기

공업화학 = Applied chemistry for engineering, v.34 no.3, 2023년, pp.279 - 284

육가은 (금오공과대학교 화학공학과) , 장지웅 (금오공과대학교 화학공학과)

초록
AI-Helper

등온 적정 열량계는 리간드-수용체 사이의 킬레이션 결합 반응의 엔탈피, 깁스에너지, 엔트로피, 화학양론 등 포함한 모든 열역학적 정보를 측정하는데 유용한 기술이다. 단일독립결합모델을 이용하여 Tricine과 HEPES 완충용엑에서의 BaCl₂ 와 ethylenediaminetetraacetic acid (EDTA)의 킬레이션 결합에서의 열역학적 정보를 획득하였다. 등온 적정 열량계를 이용하여 pH 7~11 영역에서의 킬레이션 결합의 메커니즘과 최적의 결합 조건을 확인하였다. BaCl₂와 EDTA의 결합은 자발적인 발열반응이며 pH가 증가할수록 엔트로피적 영향이 높아진다. 1:1로 결합하는 pH 영역은 pH 9.0 근처에서 매우 좁은 영역에서 나타난다.

Abstract ▼ AI-Helper

Isothermal titration calorimetry (ITC) is a useful technique to obtain thermodynamic binding properties such as enthalpy, Gibbs free energy, entropy, and stoichiometry of the chelation reaction. A single independent binding site model was used to evaluate the thermodynamic binding properties in BaCl2 and ethylenediaminetetraacetic acid (EDTA) in Trince and HEPES buffers. ITC enables us to elucidate the binding mechanism and find an optimal chelation condition for BaCl2 and EDTA in the pH range of 7~11. Chelation of BaCl2 and EDTA is a spontaneous endothermic reaction. As pH increased, entropic contributions dominated. The optimal pH range is narrow around pH 9.0, where 1:1 binding between BaCl2 and EDTA occurs.

주제어

표/그림 (7)

그림 Figure 1. Heat flow and integrated heats of chelation of BaCl₂ and EDTA in Tricine at pH 8.0 at 25 °C.
그림 Figure 2. Influence of pH on thermodynamic binding properties of chelation of BaCl₂ and EDTA in Tricine at 25 °C: (a) enthalpy, (b) Gibbs free energy, (c) entropy, and (d) stoichiometry.
표 Table 1. Thermodynamic binding parameters of chelation of BaCl₂ and EDTA depending on pH in Tricine^a
표 Table 2. Quadratic fitting parameters of thermodynamic binding parameters chelation of BaCl₂ and EDTA depending on pH in Tricine^a
그림 Figure 3. Influence of pH on thermodynamic binding properties of chelation of BaCl₂ and EDTA in HEPES at 25 °C: (a) enthalpy, (b) Gibbs free energy, (c) entropy, and (d) stoichiometry.
표 Table 3. Thermodynamic Binding Parameters of Chelation of BaCl₂ and EDTA depending on pH in HEPES^a
표 Table 4. Quadratic Fitting Parameters of Thermodynamic Binding Parameters Chelation of BaCl₂ and EDTA Depending on pH in HEPES^a

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