[논문]사물탕(四物湯) 가(加) 단참(丹參)의 상처 치료에 대한 효과

이은별; 김현지; 김채영; 최지수; 우창훈; 김영준; 안희덕

doi:10.18325/jkmr.2023.33.2.1

사물탕(四物湯) 가(加) 단참(丹參)의 상처 치료에 대한 효과
The Effects of Samul-tang-ga-dansam for Wound Healing 원문보기

Journal of Korean Medicine Rehabilitation : JKMR = 한방재활의학과학회지, v.33 no.2, 2023년, pp.1 - 18

이은별 (대구한의대학교 한의과대학 한방재활의학교실) , 김현지 (대구한의대학교 부속 대구한방병원 한방재활의학과) , 김채영 (대구한의대학교 부속 대구한방병원 한방재활의학과) , 최지수 (대구한의대학교 부속 대구한방병원 한방재활의학과) , 우창훈 (대구한의대학교 한의과대학 한방재활의학교실) , 김영준 (대구한의대학교 한의과대학 한방재활의학교실) , 안희덕 (대구한의대학교 한의과대학 한방재활의학교실)

Abstract ▼ AI-Helper

Objectives The purpose of this study was to evaluate the antioxidant, anti-inflammatory and wound healing effects of Samul-tang-ga-dansam water extract (SD) in wound-induced mice. Methods The mice were divided into five groups (n=7): the normal group, the control group, the positive control group, the low-dose SD group and the high-dose SD group. The normal group had no wounds and the other groups were wounded on the back with a leather punch. Distilled water was administered to the control group, 200 mg/kg of vitamin E was administered to the positive control group. In the low-dose SD group and the high-dose SD group, 1.23 g/kg and 2.47 g/kg of SD were administered, respectively. Antioxidant and anti-inflammatory protein levels were evaluated using western blot analysis. Skin tissue was analyzed by H&E, Masson's trichrome staining method. Results Oral administration of the SD significantly reduced the visible skin damage and decreased the reactive oxygen species and ONOO- activity of the serum. It significantly increased heme oxygenase-1, superoxide dismutase, catalase, GPx-1/2, Nrf2 and Keap-1 which are antioxidant-related factors in skin tissue and reduced NF-κB p65, inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor α, interleukin (IL)-1β, IL-6 which are inflammation-related factors. Also, SD significantly decreased NOX2, p22phox and p47phox and increased α-smooth muscle actin and COL1A1 protein expression in fibroblasts involved in connective tissue repair. According to histological examination, the thickened epithelial layer was thinned and collagen fibers were increased to accelerate wound healing. Conclusions It is suggested that Samul-tang-ga-dansam has antioxidant and anti-inflammatory effects and promotes wound tissue repair.

주제어

표/그림 (18)

표 Table I. Composition of Samul-tang-ga-dansam
표 Table II. Total Polyphenol and Total Flavonoid Contents of SD
그림 Fig. 1. DPPH free radical scavenging activity of SD. Values are presented as mean±standard error of the mean of triplicate experiments. DPPH: 2,2-Diphenyl-1-picrylhydrazyl, SD: Samultang- ga-dansam water extract.
그림 Fig. 2. ABTS radical scavenging activity of SD. Values are presented as mean±standard error of the mean of triplicate experiments. ABTS: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), SD: Samul-tang-ga-dansam water extract.
그림 Fig. 3. The levels of (A) ROS and (B) ONOO^- in serum of wound-induced model upon SD treatment. The values were presented as mean±standard deviation (n=7 for each group). SD: Samul-tang-ga-dansam water extract, ROS: reactive oxygen species, ONOO^-: peroxynitrite, VitE: vitanin E, SDL: Samul-tang-ga-dansam water extract low, SDH: Samul-tang-ga-dansam water extract high. ^###p<0.001 vs. the normal group. **p<0.01 vs. the control group. ***p<0.001 vs. the control group (ANOVA followed by least significant difference post-test).
그림 Fig. 4. The levels of pro-inflammatory in serum of wound-induced model upon SD treatment. Levels of (A) TNF-α, (B) IL-1β, and (C) IL-6 in serum measured by ELISA. The values were presented as mean±standard deviation (n=7 for each group). SD: Samul-tang-ga-dansam water extract, TNF-α: tumor necrosis factor α, IL: interleukin, VitE: vitanin E, SDL: Samul-tang-ga-dansam water extract low, SDH: Samul-tang-ga-dansam water extract high. ^###p<0.001 vs. the normal group. ***p<0.001 vs. the control group (ANOVA followed by least significant difference post-test).
그림 Fig. 5. The expression levels of NADPH oxidases in wound-induced model upon SD treatment. Expression of (A) NOX2, (B) p22^phox, and (C) p47^phox in skin measured by western blot. The values were presented as mean±standard deviation (n=7 for each group). SD: Samul-tang-ga-dansam water extract, VitE: vitanin E, SDL: Samul-tang-ga-dansam water extract low, SDH: Samul-tangga- dansam water extract high. ^###p<0.001 vs. the normal group. *p<0.05, **p<0.01, and ***p<0.001 vs. the control group (ANOVA followed by least significant difference post-test).
그림 Fig. 6. The expression levels of Nrf2 and Keap1 in wound-induced model upon SD treatment. Expression of (A) Nrf2 and (B) Keap1 in skin measured by western blot. The values were presented as mean±standard deviation (n=7 for each group). SD: Samul-tangga- dansam water extract, VitE: vitanin E, SDL: Samul-tang-ga-dansam water extract low, SDH: Samul-tang-ga-dansam water extract high. ^###p<0.001 vs. the normal group. **p<0.01 and ***p<0.001 vs. the control group (ANOVA followed by least significant difference post-test).
그림 Fig. 7. The expression levels of antioxidant enzymes in wound-induced model upon SD treatment. Expression of (A) HO-1, (B) SOD, (C) catalase, and (D) GPx-1/2 in skin measured by western blot. The values were presented as mean±standard deviation (n=7 for each group). SD: Samul-tang-ga-dansam water extract, HO-1: heme oxygenase-1, SOD: superoxide dismutase, catalase, GPx-1/2: glutathione peroxidase-1/2, VitE: vitanin E, SDL: Samul-tang-ga-dansam water extract low, SDH: Samul-tang-ga-dansam water extract high. ^###p<0.001 vs. the normal group. *p<0.05, **p<0.01, and ***p<0.001 vs. the control group (ANOVA followed by least significant difference post-test).
그림 Fig. 7. Continued.
그림 Fig. 8. The expression levels of NF-κB p65, iNOS and COX-2 in wound-induced model upon SD treatment. Expression of (A) NF-κB p65, (B) iNOS, and (C) COX-2 in skin measured by western blot. The values were presented as mean±standard deviation (n=7 for each group). SD: Samul-tang-ga-dansam water extract, NF-κB p65: nuclear factor kappa-light-chain-enhancer of activated B cells p65, iNOS: inducible nitric oxide synthase, COX-2: cyclooxygenase-2, VitE: vitanin E, SDL: Samul-tang-ga-dansam water extract low, SDH: Samul-tang-ga-dansam water extract high. ^###p<0.001 vs. the normal group. *p<0.05, **p<0.01, and ***p<0.001 vs. the control group (ANOVA followed by least significant difference ost-test).
그림 Fig. 9. The expression levels of pro-inflammatory cytokines in wound-induced model upon SD treatment. Expression of (A) TNF-α, (B) IL-1β, and (C) IL-6 in skin measured by western blot. The values were presented as mean±standard deviation (n=7 for each group). SD: Samul-tang-ga-dansam water extract, TNF-α: tumor necrosis factor α, IL: interleukin, VitE: vitanin E, SDL: Samultang- ga-dansam water extract low, SDH: Samul-tang-ga-dansam water extract high. ^###p<0.001 vs. the normal group. *p<0.05, **p<0.01, and ***p<0.001 vs. the control group (ANOVA followed by least significant difference post-test).
그림 Fig. 11. The wounded area at each group over time. The values were presented as mean±standard deviation (n=7 for each group). VitE: vitanin E, SDL: Samul-tang-ga-dansam water extract low, SDH: Samul-tang-ga-dansam water extract high.
그림 Fig. 10. The expression levels of α-SMA and COL1A1 in wound-induced model upon SD treatment. Expression of (A) α-SMA and (B) COL1A1 in skin measured by western blot. The values were presented as mean±standard deviation (n=7 for each group). SD: Samul-tang-ga-dansam water extract, α-SMA: α-smooth muscle actin, COL1A1: collagen type I alpha 1, VitE: vitanin E, SDL: Samul-tang-ga-dansam water extract low, SDH: Samul-tang-ga-dansam water extract high. ^###p<0.001 vs. the normal group. *p<0.05, **p<0.01, and ***p<0.001 vs. the control group (ANOVA followed by least significant difference post-test).
그림 Fig. 12. The representative photomicrographs of skin sections stained by H&E staining. VitE: vitanin E, SDL: Samul-tang-gadansam water extract low, SDH: Samul-tang-ga-dansam water extract high.
그림 Fig. 13. The representative photomicrographs of skin sections stained by Masson’s trichrome staining. VitE: vitanin E, SDL:Samul-tang-ga-dansam water extract low, SDH: Samul-tang-ga-dansam water extract high.
그림 Fig. 14. The levels of (A) AST and (B) ALT in serum of wound-induced model upon SD treatment. SD: Samul-tang-ga-dansam water extract, AST: aspartate aminotransferase, ALT: alanine aminotransferase, VitE: vitanin E, SDL: Samul-tang-ga-dansam water extract low, SDH: Samul-tang-ga-dansam water extract high. The values were presented as mean±standard deviation (n=7 for each group). ^#p<0.05 vs. the normal group. *p<0.05 vs. the control group (ANOVA followed by least significant difference post-test).
표 Table III. Body Weight Change

참고문헌 (34)

The National College of Oriental Medicine Dermatology？Textbook Compilation Committee. Text of Korean？dermatology. Sunwoo Publisher. 2007:37-57, 72, 127-8,？271-3.
The Korean Society of Plastic and Reconstructive Surgeons.？Standard plastic surgery. Koonja Publishing. 2009:83-91,？453-62.
Yeon CH, Roh SS. Effects of SaMulTangGamibang？(SMTG) on the inflammatory reactions. The Journal of？Korean Medicine Ophthalmology and Otolaryngology？and Dermatology. 2004;17(3):18-25.
The National College of Oriental Medicine Herbology？Textbook Compilation Committee. Medicinal herbology.？Younglim-Sa. 2016:458.
Kim JM, Lee IC, Lee TJ, Kim BH. The promoting effect of Dansam-Samultang on hair growth by gross and？histological observation. Journal of Investigative Cosmetology.？2001;7(3):257-65.
Yoo JY, Kim YC, Lee JH, Woo HJ. The effect of？DanSamSaMul-Tang on hematopoiesis. Journal of Korean？Medicine. 2022;23(1):145-55.
Lee IC, Kim BH, Kim SO, Kim MK. Skin safety evaluation of a Korean traditional prescription, Dansam-samultang and Samwhang-sasim-tang. Journal of Physiology &？Pathology in Korean Medicine. 2011;25(2):212-6.
Lee SE. Anti-obesity effect and action mechanism of？Dansam-Samultang in C57BL/6 obese mice fed high fat？diet. The Korean Society of Beauty and Art. 2018;19(2):111-23.
Musalmah M, Fairuz AH, Gapor MT, Wan Ngah WZ.？Effect of vitamin E on plasma malondialdehyde, antioxidant enzyme levels and the rates of wound closures？during wound healing in normal and diabetic rats. Asia？Pacific Journal of Clinical Nutrition. 2002;11:S448-51.

상세보기
Heo J. Donguibogam. Donguibogam Publishing. 2010:45-6, 2162.
Rama P, Vignesh A, Lakshmanan G, Murugesan K. In？vitro antioxidant activity of Achyranthes aspera Linn.？International Journal of Medicine and Pharmacy. 2013;3(2):67-78.
Chang CC, Yang MH, Wen HM, Chern JC. Estimation？of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug？Analysis. 2002;10(3):178-82.
Blosis MS. Antioxidant determinations by the use of？a stable free radical. Nature. 1958;181:1199-200.

상세보기
Re R, Pellegrini N, Proteggente A, Pannala A, Yang？M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free？Radical Biology and Medicine. 1999;26(9-10):1231-7.
Kim HY, Hong SU. The effects of Gamisipjeontang-gamibang on injured tissue after burn elicitation. The Journal？of Korean Medicine Ophthalmology and Otolaryngology？and Dermatology. 2009;22(1):62-75.
Kim JE, Chae KY, Park SN. Antioxidative and inhibitory activities on tyrosinase of Hippophae rhamnoides leaf extracts. Journal of the Society of Cosmetic？Scientists of Korea. 2011;37(3):265-73.
Lee KT, Yu BC, Kim YS, Seol IC. A prior study on？the effect of Samul-tang to regeneration of injured peripheral nerve fiber. Journal of Haehwa Medicine. 2005;14(2):107-12.
Rice-evans CA, Miller NJ, Bolwell PG, Bramley PM,？Pridham JB. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radical Research.？1995;22(4):375-83.

상세보기
Dai J, Mumper RJ. Plant phenolics: extraction, analysis,？and their antioxidant and anticancer properties. Molecules.？2010;15(1):7313-52.
Kim ES, Jang HD, Kim GN. Anti-oxidative function of？fisetin and its potential as an anti-oxidant nutri-cosmetics.？Korean Journal of Aesthetics and Cosmetology. 2012;10(3):515-21.
Kim EJ, Choi JY, Yu MR, Kim MY, Lee SH, Lee BH.？Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants.？Korean Journal of Food Science and Technology. 2012;44(3):337-42.
Del R, Luis A. ROS and RNS in plant physiology: an？overview. Journal of Experimental Botany. 2015;66(10):2827-37.
Kang SW. Role of reactive oxygen species in cell death？pathways. Hanyang Medical Reviews. 2013;33(2):77-82.
Kumar V, Abbas AK, Aster JC. Robbins and cotran？pathologic basis of disease. Panmun Education. 2017:45-6, 76-8, 80-4.
Abdalla HB, Napimoga MH, Lopes AH, de Macedo？MAG, Cunha TM, Van DTE, Napimoga JTC. Activation？of PPAR-γ induces macrophage polarization and reduces neutrophil migration mediated by heme oxygenase 1. International immunopharmacology. 2020;84:106565.
El-Benna J, Dang PMC, Gougerot-Pocidalo MA, Marie？JC, Braut-Boucher F. P47phox, the phagocyte NADPH？oxidase/NOX2 organizer: structure, phosphorylation？and implication in diseases. Experimental & Molecular？Medicine. 2009;41(4):217-25.

상세보기
Tarafdar A, Pula G. The role of NADPH oxidases and？oxidative stress in neurodegenerative disorders. International？Journal of Molecular Sciences. 2018;19(12):3824.

상세보기
Nam JW, Lee YS. Role of NADPH oxidase in the mechanism of arachidonic acid-induced apoptosis in HepG2？human hepatoblastoma cells. Yakhak Hoeji. 2012;56(2):80-5.
Kaspar JW, Niture SK, Jaiswal AK. Nrf2: INrf2(Keap1)？signaling in oxidative stress. Free Radical Biology and？Medicine. 2009;47(9):1304-9.

상세보기
Abbas AK, Lichtman AH, Pillai S. Cellular and molecular？immunology. 9th ed. Panmun Education. 2016:85-94,？189-91.
Kwon DJ, Ju SM, Youn GS, Choi SY, Park J. Suppression？of iNOS and COX-2 expression by flavokawain A via？blockade of NF-κB and AP-1 activation in RAW 264.7？macrophages. Food and Chemical Toxicology. 2013;58:479-86.
Ko UH, Hong J, Shin H, Kim CW, Shin JH. Combinatorial？physical stimulation and synergistically-enhanced fibroblast differentiation for skin regeneration. Journal of the？Korean Society for Precision Engineering. 2015;32(8):755-60.
Almeida L, Oliveira J, Guimaraes LH, Carvalho EM,？Blackwell JM, Castellucci L. Wound healing genes and？susceptibility to cutaneous leishmaniasis in Brazil: role？of COL1A1. Infection, Genetics and Evolution. 2015;30:225-9.

상세보기
The Text Compilation Committee of Korean Dermatological？Association. Dermatology. 5th ed. Yeo Moon Gak. 2008:27.

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증