[논문]미세먼지 PM10으로 손상을 유도한 각질형성세포에서 청각 (Codium fragile) 추출물의 항노화 효과

김보애

doi:10.6116/kjh.2023.38.4.45

[국내논문] 미세먼지 PM10으로 손상을 유도한 각질형성세포에서 청각 (Codium fragile) 추출물의 항노화 효과
Anti-aging effect of Codium fragile extract on keratinocytes damaged by fine dust PM10 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.38 no.4, 2023년, pp.45 - 52

Abstract ▼ AI-Helper

Objectives : Fine dust caused by environmental pollution cause oxidative damage and skin aging. In this study, The possibility of using the Codium fragile extract (CFE) as an anti-aging product for skin improvement was evaluated by confirming the protective effect of skin cells from PM10 (particulate matter 10) through inhibition of ROS and MMPs. Methods : In this study, elastase and collagenase inhibitory activities were evaluated. Cell viability was evaluated by treating keratinocytes (HaCaT cell line) with CFE at various concentrations. The cytoprotective effect from PM10 in keratinocyteswas evaluated using the 3-[4,5-dimethylthiazol]-2-yl]-2,5-diphenyl-tetrazoliumbromide (MTT) assay. ROS (reactive oxygen species) was measured in keratinocytes damaged by PM10 using DCF-DA (2′,7′-dichlorofluorescin diacetate) fluorescence staining. As an anti-aging effect of CFE, MMP-1 (matrix metalloproteinase-1) and MMP-1 (matrix metalloproteinase-9) inhibitory activities were evaluated. Results : As a result, CFE decreased the activity of elastase and collagenase. As a result of evaluating the toxicity of CFE, it is non-toxic at a concentration of 10 to 80 ㎍/㎖. Although cell viability of HaCaT cells treated with PM10 decreased, cell viability increased by 38% when treated with CFE 80 ㎍/㎖. Also, ROS decreased by 8.4%, and MMP-1 and MMP-9 decreased at CFE 80 ㎍/㎖. Conclusions : CFE showed excellent cell protection effect, and it is considered that it can be used in anti-aging products for skin improvement by effectively inhibiting ROS and MMPs from keratinocyte damage caused by fine dust.

주제어

표/그림 (5)

그림 Figure 1. Elastase inhibitory activity of ethanol extract from Codium fragile (CFE). Data shown is from representative experiment repeated three times with similar results. The values are the mean±standard error of mean *** p<0.001 compared the control group.
그림 Figure 2. Collagenase inhibitory activity of ethanol extract from Codium fragile (CFE). Data shown is from representative experiment repeated three times with similar results. The values are the mean ±standard error of mean *** p<0.001 compared the control group.
그림 Figure 3. Protective effect of ethanol extract from Codium fragile (CFE) on the PM10 induced cytotoxicity. HaCaT cells were exposed to PM10 (50 ㎍/㎖) and treated with various concentrations (10, 20, 40, 80 ㎍/㎖) of CFE. The data is expressed as percentage cell viability and represent the means. (a) Cell viability with various concentrations of CFE, (b) Protective effect of CFE on keratinocytes damaged by PM10. Data shown are from representative experiment repeated three times with similar results. The values are the mean±standard error of mean. ### p<0.001 as compared the control group, ** p<0.01, *** p<0.001 compared with the PM10- treated control group.
그림 Figure 4. Cellular antioxidant activity of ethanol extract from Codium fragile (CFE) in PM10 induced oxidative stress in HaCaT cells. Intracellular reactive oxygen species (ROS) levels generated by PM10 were detected using a spectrofluorometer after DCF-DA staining. Data shown is from representative experiment repeated three times. The values are the mean±standard error of mean. ### p<0.001 as compared the control group, ** p<0.01 compared with the PM10-treated control group.
그림 Figure 5. Effects of ethanol extract from Codium fragile (CFE) into matrix metalloproteinase (MMP-1 and MMP-9) secretion by PM10 induced HaCaT cells. Cells were treated with CFE before PM10 exposure. The level of secretion of (a) MMP-1, (b) MMP-9 were measured in the culture medium of PM10 treated HaCaT cells. The values are the mean±standard error of mean. ### p<0.001 as compared the control group, ** p<0.01, *** p<0.001 compared with the PM10-treated control group.

참고문헌 (40)

Mukherjee A. Agrawal M. World air particulate matter:？Sources, distribution and health effects. Environ.？Chem. Lett. 2017 ; 15 : 283-309. https://doi.org/10.1007/s10311-017-0611-9？

상세보기
Ngoc LTN, Park D, Lee Y, Lee YC. Systematic Reviewand Meta-Analysis of Human Skin Diseases Due to？Particulate Matter. Int. J. Environ. Res. Public？Health. 2017 ; 14 : 1458-69. https://doi.org/10.3390/ijerph14121458？

상세보기
Kim KE, Cho D, Park HJ. Air pollution and skin？diseases: Adverse effects of airborne particulate matter on various skin diseases. Life Sci. 2016 ; 152 :？126-34. https://doi.org/10.1016/j.lfs.2016.03.039？

상세보기
Krzyzanowski M. WHO Air Quality Guidelines for？Europe. J. Toxicol. Environ. Health A. 2008 ; 71(1) :？47-50. https://doi.org/10.1080/15287390701557834？

상세보기
Fuzzi S, Baltensperger U, Carslaw K, Decesari S, Der？GHD, Facchini MC. Fowler DK, Langford B, Lohmann？U. Particulate matter, air quality and climate:？Lessons learned and future needs. Atmos. Chem.？Phys. 2015 ; 15(14) : 8217-99. https://doi.org/10.5194/acp-15-8217-2015？

상세보기
Zhu X, Qiu H, Wang L, Duan Z, Yu H, Deng R, Zhang？Y, Zhou L. Risks of hospital admissions from a spectrum of causes associated with particulate？matter pollution. Sci. Total Environ. 2019 ; 656 :？90-100. https://doi.org/10.1016/j.scitotenv.2018.11.240？

상세보기
Lee CW, Lin ZC, Hsu LF, Fang Y, Chiang YC, Tsai MH,？Lee MH, Li SY, Hu SC, Lee IT. Eupafolin ameliorates？COX-2 expression and PGE2 production in？particulate pollutants-exposed human keratinocytes？through ROS/MAPKs pathways. J. Ethnopharmacol.？2016 ; 189 : 300-9. https://doi.org/10.1016/j.jep.2016.05.002？

상세보기
Ha JW, Song H, Hong SS, Boo YC. Marine Alga？Ecklonia cava Extract and Dieckol Attenuate？Prostaglandin E2 Production in HaCaT Keratinocytes？Exposed to Airborne Particulate Matter. Antioxidants.？2019 ; 8(6) : 190-5. https://doi.org/10.3390/antiox8060190？

상세보기
Boo YC. Can Plant Phenolic Compounds Protect the？Skin from Airborne Particulate Matter?. Antioxidants.？2019 : 8(9) : 379-97. https://doi.org/10.3390/antiox8090379？

상세보기
Bae IA, Ha JW, Choi JY, Boo YC. Antioxidant Effects？of Korean Propolis in HaCaT Keratinocytes Exposed？to Particulate Matter 10. Antioxidants. 2022 ; 11(4)？: 781-99. https://doi.org/10.3390/antiox11040781？

상세보기
Kim KE, Cho D, Park HJ. Air pollution and skin？diseases: adverse effects of airborne particulate？matter on various skin diseases. Life Sci. 2016 ; 152 :？126-34. https://doi.org/10.1016/j.lfs.2016.03.039？

상세보기
Chieosilapatham P, Kiatsurayanon C, Umehara, JV,？Trujillo-Paez, Peng GHY, Nguyen LTH, Niyonsaba？F. Keratinocytes: innate immune cells in atopic？dermatitis. Clin. Exp. Immunol. 2021 ; 204(3) : 296-309. https://doi.org/10.1111/cei.13575？

상세보기
Lee JB, Ohta Y, Hayashi KT. Immunostimulating？effects of sulfated galactan from Codium fragile.？Carbohydr Res. 2010 ; 345(10) : 1452-4. https://doi.org/10.1016/j.carres.2010.02.026？

상세보기
Oh YS, Lee IK, Boo SM. An annotated account of？Korean economic seaweeds for food, medical and？industrial uses. Algae. 1990 ; 5(1) : 57-71.？
Chengkui Z, Tseng CK, Junfu Z, Chang CF. Chinese？seaweeds in herbal medicine. Hydrobiologia. 1984？; 116 : 152-4.？

상세보기
Kang CH, Choi YH, Park SY, Kim GY. Antiinflammatory effects of methanol extract of Codium？fragile in lipopolysaccharide-stimulated RAW 264.7？cells. J. Med. Food. 2012 ; 15(1) : 44-50. https://doi.org/10.1089/jmf.2010.1540？

원문보기 상세보기
Cho KJ, Lee YS, Ryu BH. Antitumor effect and？immunology activity of seaweeds toward sarcoma-180. Korean J. Fish. Aqua. Sci. 1990 ; 23(5) : 345-352.？
Rogers DJ, Jurd KM, Blunden G, Paoletti S. Zanetti？F. Anticoagulant activity of a proteoglycan in？extracts of Codium Fragile sp. atlanticum. J. Appl.？Phycol. 1990 ; 2 :357-61.？
Rogers DJ, Loveless RW. Electron microscopy of？human erythrocytes aglutinated by lectin from Codium？fragile sp. tomentosoides and pseudohaemagglutinin？from Ascophyllum nodosum. J. Appl. Phycol. 1991？; 3 : 83-6.？
Lee AR, Kim SH, Kim SJ, Kim KJ, Kwon O, Choi？JY, Roh SS. Anti-skin-aging Effect of Mori Folium？through decreased Advanced glycation end product？(AGEs). Kor. J. Herbol. 2017；32(5):7-12. http://dx.doi. org/10.6116/kjh.2017.32.5.7.？
Lee EH, Cho YJ. Elevation of anti-oxidative activity？and inhibitory activities against tyrosinase, elastase,？collagenase and hyaluronidase of Oplismenus？undulatifolius by elicitor treatment. J Appl Biol Chem.？2020 ; 63(3) : 221-7. https://doi.org/10.3839/jabc.2020.030？

원문보기 상세보기
Kraunsoe JA, Claridge TD, Lowe G. Inhibition of？human leukocyte and porcine pancreatic elastase？by homologues of bovine pancreatic trypsin inhibitor.？Biochemistry. 1996 ; 35 : 9090-6. https://doi.org/10.1021/bi953013b？

상세보기
Kim CJ, Shim JK, Kwon KC, Lim JD, Choi SK, Yu？CY, Lee JG. Changes in Non-saponin Fatty Acid？Content and Increases in Inhibitory Activities of？Collagenase and Elastase by Treatment with？Saccharomyces cerevisiae of the Supercritical Fluid？Extracted Oil of the Adventitious Roots Culture of？Wild Mountain Ginseng Korean. J. Medicinal Crop？Sci. 2018 ; 26(2) : 170-80. http://dx.doi.org/10.7783/KJMCS.2018.26.2.170？

원문보기 상세보기
Wunsch E, Heidrich HG. For the quantitative？determination of collagenase. Hoppe-Seyler's Physiol？Chem. 2009 ; 333 : 149-51. https://doi.org/10.1515/bchm2.1963.333.1.149？

상세보기
Seok JK, Lee JW, Kim YM, Boo YC. Punicalagin？and (-)-epigallocatechin-3-gallate rescue cell？viability and attenuate inflammatory responses of？human epidermal keratinocytes exposed to airborne？particulate matter PM10. Skin Pharmacol. Physiol.？2018 ; 31(3) : 134-43. https://doi.org/10.1159/000487400？

상세보기
Park JY, Hwang J, Yun JK, Han KH, Do EJ, Kim？Sung OK, Kim MR. Effect of Ssanghwa-tang？Extract on Antioxidant and Anti-aging Enzyme？Activities. Kor. J. Herbol. 2012；27(3):67-74.？https://doi.org/10.6116/kjh.2012.27.3.67？

원문보기 상세보기
Youn SN, Kim YJ, Lee YJ, Kim MR, Yoo WK. Effect？of ethanol extract from mixture including Angelicae？Dahuricae Radix on Dermal Anti-aging and？Whitening. Kor. J. Herbol. 2019；34(6):109-15.？http://dx.doi.org/10.6116/kjh.2019.34.6.109.？
Federico A, Morgillo, Tuccillo FC, Ciardiello F,？Loguercio C. Chronic inflammation and oxidative？stress in human carcinogenesis. Int. J. Cancer.？2007 ; 121(11) : 2381-6. https://doi.org/10.1002/ijc.23192？

상세보기
Choi MK, Kim J, Park HM, Lim CM, Pham TH, Ha？Shin Y, Kim SE, Oh DK, Yoon DY. The DPA-derivative 11S, 17S-dihydroxy 7,9,13,15,19 (Z,E,Z,E,Z)-docosapentaenoic acid inhibits IL-6 production by？inhibiting ROS production and ERK/NF-κB pathway？in keratinocytes HaCaT stimulated with a fine dust？PM10. Ecotoxicology and Environmental Safety. 2022 ; 232 : 113252. https://doi.org/10.1016/j.ecoenv.2022.113252？

상세보기
Kim MS, Kim MK, Han DH, Ko K, Kim SY.？Antibacterial Activity and other Functions of Codium？fragile and Chaenomeles sinensis Extracts by？Extraction Method. Korean S. Bio Journal. 2018 ;？33(2) : 89-94. http://dx.doi.org/10.7841/ksbbj.2018.33.2.89？

상세보기
Shin DB, Han EH, Park SS. Cytoprotective Effects？of Phaeophyta Extracts from the Coast of Jeju？Island in HT-22 Mouse Neuronal Cells. J Korean？Soc Food Sci Nutr. 2014 ; 42(2) : 224-30.？http://dx.doi.org/10.3746/jkfn.2014.43.2.224？

원문보기 상세보기
Kolsi RBA, Salah HB, Hamza A, Elfeki A, Belguith？K. Characterization and evaluating of antioxidant？and antihypertensive properties of green alga？(Codium fragile) from the coast of Sfax. Journal of？Pharmacognosy and Phytochemistry. 2017 ; 6(2) :？186-91.？
Lee JH, Kim BA. A Study on Seaweed Sea Staghorn？(Codium fragile) Ethanol Extract for Antioxidant.？The journal of Convergence on Culture Technology.？2019 ; 5(4) : 467-72. https://doi.org/10.17703/JCCT.2019.5.4.467？

원문보기 상세보기
Lee C, Park GH, Ahn EM, Kim BA, Park CI, Jang？JH. Protective effect of Codium fragile against？UVB-induced pro-inflammatory and oxidative？damages in HaCaT cells and BALB/c mice.？Fitoterapia. 2013 ; 86 : 54-63. https://doi.org/10.1016/j.fitote.2013.01.020？

상세보기
Bailly C, El MB, Corbineau HF. From intracellular？signaling networks to cell death: The dual role of？reactive oxygen species in seed physiology. Comptes？Rendus. Biol. 2008 ; 331(10) : 806-14. https://doi.org/10.1016/j.crvi.2008.07.022？

상세보기
Vurusaner B. Poli G. Basaga H. Tumor suppressor？genes and ROS: Complex networks of interactions.？Free. Radic. Biol. Med. 2012 ; 52(1) : 7-18. https://doi.org/10.1016/j.freeradbiomed.2011.09.035？

상세보기
Dai J, Ma H, Fan J, Li Y, Wang J, Ni H, Xia G,？Chen S. Crude polysaccharide from an anti-UVB？cell clone of Bupleu-rum scorzonerifolium protect？HaCaT cells against UVB-induced oxidative stress.？Cytotechnology. 2011 ; 63(6) : 599-607. https://doi.org/10.1007/s10616-011-9381-6？

상세보기
Seok JK, Choi MA, Ha JW, Boo YC. Role of Dual？Oxidase 2 in Reactive Oxygen Species Production？Induced by Airborne Particulate Matter PM10 in？Human Epidermal Keratinocytes. J. Soc. Cosmet.？Sci. Korea. 2019 ; 45(1) : 57-67. https://doi.org/10.15230/SCSK.2019.45.1.57？

원문보기 상세보기
Cho S, Kim HH, Lee MJ, Lee S, Park CS, Nam SJ,？Han JJ, Kim JW, Chung JH. Phosphatidylserine？prevents UV-induced decrease of type I procollagen？and increase of MMP-1 in dermal fibroblasts and？human skin in vivo. J. Lipid Res. 2008 ; 49(6) :？1235-1245. https://doi.org/10.1194/jlr.M700581-JLR200？

상세보기
Jang YA, Kim BA. Protective Effect of Spirulina-Derived C-Phycocyanin against Ultraviolet B-Induced？Damage in HaCaT Cells. Medicina. 2021 ; 16(3) :？273-85. https://doi.org/10.3390/medicina57030273

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증