[논문]금전초에서 분리한 cynaroside의 신경세포보호 활성

류가희; 마충제

doi:10.22889/kjp.2023.54.1.9

금전초에서 분리한 cynaroside의 신경세포보호 활성
Neuroprotective Activity of Cynaroside Isolated from Lysimachia christinae 원문보기

생약학회지, v.54 no.1, 2023년, pp.9 - 15

류가희 (강원대학교 의생명과학대학 생물의소재공학과) , 마충제 (강원대학교 의생명과학대학 생물의소재공학과)

Abstract ▼ AI-Helper

In the previous study, we reported that cynaroside isolated from Lysimachia christinae methanolic extract had potent neuroprotective activities in neuronal cell death injured by excessive glutamate. In this study, we tried to confirm the neuroprotective activities of cynaroside in glutamate injured HT22 cells and establish mechanisms of neuroprotective action of cynaroside. We employed HT22 cells damaged by glutamate-induced cell death as a bioassay system. Cynaroside decreased reactive oxygen species increased by excessive glutamate treatment in HT22 cells. Also, Ca2+ concentration was decreased by cynaroside treatment. Cynaroside restored mitochondrial membrane potential to normal condition. It also increased not only glutathione reductase but also peroxidase to the control level. And it increased amount of glutathione, an endogenous antioxidant. These results suggested that cynaroside isolated from L. christinae showed potent neuroprotective activity through the anti-oxidative pathway.

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표/그림 (8)

그림 Fig. 1. Structure of cynaroside isolated from L. christinae.
그림 Fig. 2. Effect of cynaroside (1.0, 10.0 and 100.0 mM) on glutamate- induced death of HT22 cells. Data expressed as mean ± the standard error of the mean. *p<0.05, **p<0.01, ***p<0.001 versus the glutamate-treated group.
그림 Fig. 3. Effect of cynaroside (1.0, 10.0 and 100.0 mM) on reactive oxygen species production in glutamate injured HT22 cells. Data expressed as mean ± the standard error of the mean. *p<0.05, **p<0.01, ***p<0.001 versus the glutamate-treated group.
그림 Fig. 4. Effect of cynaroside (1.0, 10.0 and 100.0 mM) on calcium ion influx in glutamate injured HT22 cells. Data expressed as mean ± the standard error of the mean. *p<0.05, **p<0.01, ***p<0.001 versus the glutamate-treated group.
그림 Fig. 5. Effect of cynaroside (1.0, 10.0 and 100.0 mM) on glutamate- induced disruption of mitochondrial membrane potential in HT22 cells. Data expressed as mean ± the standard error of the mean. *p<0.05, **p<0.01, ***p<0.001 versus the glutamate- treated group.
그림 Fig. 6. Effect of cynaroside (1.0, 10.0 and 100.0 mM) on glutathione level in glutamate injured HT22 cells. Data expressed as mean ± the standard error of the mean. *p<0.05, **p<0.01, ***p<0.001 versus the glutamate-treated group.
그림 Fig. 7. Effect of cynaroside (1.0, 10.0 and 100.0 mM) on glutathione reductase in glutamate injured HT22 cells. Data expressed as mean ± the standard error of the mean. *p<0.05, **p<0.01, ***p<0.001 versus the glutamate-treated group.
그림 Fig. 8. Effect of cynaroside (1.0, 10.0 and 100.0 mM) on glutathione peroxidase in glutamate injured HT22 cells. Data expressed as mean ± the standard error of the mean. *p<0.05, **p<0.01, ***p<0.001 versus the glutamate-treated group.

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문제 정의

본 연구에서는 glutamate를 독성물질로 처리한 HT22 세포를 활성검색계로 하여 이에 대한 신경세포 보호 활성이 보고된 금전초 추출물로부터 분리 정제한 cynaroside의 신경세포 보호 활성을 다시 한번 확인하고 신경세포 보호 활성의 작용기전을 밝히고자 하였다. 이에 glutamate로 신경 독성을유도한 HT22 세포를활성 검색계로하여신경세포의 사멸과 관련된 바이오 마커의 변화를 평가하여 cynaroside의 약리 작용 기전을 규명하는 연구를 수행하였다.
본 연구에서는 glutamate를 독성물질로 처리한 HT22 세포를 활성검색계로 하여 이에 대한 신경세포 보호 활성이 보고된 금전초 추출물로부터 분리 정제한 cynaroside의 신경세포 보호 활성을 다시 한번 확인하고 신경세포 보호 활성의 작용기전을 밝히고자 하였다. 이에 glutamate로 신경 독성을유도한 HT22 세포를활성 검색계로하여신경세포의 사멸과 관련된 바이오 마커의 변화를 평가하여 cynaroside의 약리 작용 기전을 규명하는 연구를 수행하였다.

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