[논문]Ovalbumin으로 유발된 천식 동물모델에서 GGX의 효과

김태현; 양원경; 이수원; 우성천; 김승형; 박양춘

doi:10.22246/jikm.2023.44.3.294

Ovalbumin으로 유발된 천식 동물모델에서 GGX의 효과
Effects of GGX on an Ovalbumin-induced Asthma Mice Model 원문보기

대한한방내과학회지 = The journal of internal Korean medicine, v.44 no.3, 2023년, pp.294 - 312

김태현 (대전대학교 한의과대학 폐계내과학교실) , 양원경 (대전대학교 한의과대학 폐계내과학교실) , 이수원 (대전대학교 한의과대학 폐계내과학교실) , 우성천 (대전대학교 한의과대학 폐계내과학교실) , 김승형 (대전대학교 동서생명과학연구원) , 박양춘 (대전대학교 한의과대학 폐계내과학교실)

Abstract ▼ AI-Helper

Objective: The purpose of this study is to evaluate the effects of GGX on an ovalbumin (OVA)-induced asthma mice model. Methods: Balb/c mice were challenged with OVA and then treated with three concentrations of GGX (100, 200, and 400 mg/kg). After sacrifice, the bronchoalveolar lavage fluid (BALF) or lungs of the mice were analyzed by fluorescence-activated cell sorting, ELISA, real-time PCR, H&E, Masson's trichrome, PAS and AB-PAS staining, and immunohistofluorescence staining. Results: GGX significantly inhibited the increase of total cells, immune cells (lymphocyte, neutrophils, macrophage, CD4+, CD8+, CD4+CD69+, CD62L-CD44high+, Gr-1+SiglecF-), and the expression of cytokines (IL-4, IL-5, IL-13, IFN-γ) in BALF. It also significantly inhibited the increase of total cells, immune cells (lymphocyte, neutrophils, eosinophil/macrophage, CD3+, CD19+, CD3+CD193+, CD4+, CD8+, CD4+CD69+, CD62L-CD44high+, and Gr-1+SiglecF-), and the expression of IL-13, TARC, and MCP-1 in lung tissue. GGX decreased the severity of histological lung injury and the expressions of STAT3 and GATA3. Conclusion: This study suggests the probability of using GGX for the treatment of asthma by inhibiting inflammatory immune response.

주제어

표/그림 (15)

표 Table 1. The Composition of GGX
그림 Fig. 1. Experimental schedule of OVA-induced asthma mice model.
표 Table 2. Oligonucleotide Sequence Used for RT-PCR
그림 Fig. 2. Effects of GGX on BALF (A) and lung tissue (B) cell number in OVA-induced asthma mice. Mice were induced by OVA (Control), and then treated with dexamethasone 3 mg/kg (Dexa) and GGX (100, 200, 400 mg/kg). Data are presented as mean±SE (n=8).^†: Significant difference compared with normal group (^†††p<0.001), * : Significant difference compared with control group (* p<0.05, ** p<0.01, *** p<0.001).
표 Table 3. Absolute Number of Various Immune Cells in BALF of OVA-induced Asthma Mice Model Treated by GGX
표 Table 4. Absolute Number of Various Immune Cells in Lung of OVA-induced Asthma Mice Model Treated by GGX
그림 Fig. 3. Effect of GGX on IL-4 production in BALF of OVA-induced asthma mice. Mice were induced by OVA (Control), and then treated with dexamethasone 3 mg/kg (Dexa) and GGX (100, 200, 400 mg/kg). Data are presented as mean±SE (n=8). ^†: Significant difference compared with normal group (^†††p<0.001), * : Significant difference compared with control group (* p<0.05, ** p<0.01, *** p<0.001).
그림 Fig. 4. Effect of GGX on IL-5 production in BALF of OVA-induced asthma mice. Mice were induced by OVA (Control), and then treated with dexamethasone 3 mg/kg (Dexa) and GGX (100, 200, 400 mg/kg). Data are presented as mean±SE (n=8). ^†: Significant difference compared with normal group (^†††p<0.01), * : Significant difference compared with control group (* p<0.05, ** p<0.01).
그림 Fig. 5. Effect of GGX on IL-13 production in BALF of OVA-induced asthma mice. Mice were induced by OVA (Control), and then treated with dexamethasone 3 mg/kg (Dexa) and GGX (100, 200, 400 mg/kg). Data are presented as mean±SE (n=8). ^†: Significant difference compared with normal group (^††p<0.01), * : Significant difference compared with control group (* p<0.05, ** p<0.01).
그림 Fig. 6. Effect of GGX on IFN-γ production in BALF of OVA-induced asthma mice. Mice were induced by OVA (Control), and then treated with dexamethasone 3 mg/kg (Dexa) and GGX (100, 200, 400 mg/kg). Data are presented as mean±SE (n=8). ^†: Significant difference compared with normal group (_††p<0.01), * : Significant difference compared with control group (* p<0.05, ** p<0.01).
그림 Fig. 7. Effect of GGX on IL-13 mRNA expression in lung of OVA-induced asthma mice. Mice were induced by OVA (Control), and then treated with dexamethasone 3 mg/kg (Dexa) and GGX (100, 200, 400 mg/kg). Data are presented as mean±SE (n=8). ^†: Significant difference compared with normal group (^††p<0.01), * : Significant difference compared with control group (* p<0.05, ** p<0.01).
그림 Fig. 8. Effect of GGX on TARC mRNA expression in lung of OVA-induced asthma mice. Mice were induced by OVA (Control), and then treated with dexamethasone 3 mg/kg (Dexa) and GGX (100, 200, 400 mg/kg). Data are presented as mean±SE (n=8). ^†: Significant difference compared with normal group (^††p<0.01), * : Significant difference compared with control group (* p<0.05, ** p<0.01).
그림 Fig. 9. Effect of GGX on MCP-1 mRNA expression in lung of OVA-induced asthma mice. Mice were induced by OVA (Control), and then treated with dexamethasone 3 mg/kg (Dexa) and GGX (100, 200, 400 mg/kg). Data are presented as mean±SE (n=8). ^†: Significant difference compared with normal group (^†††p<0.001), * : Significant difference compared with control group (* p<0.05).
그림 Fig. 10. Effect of GGX on histophathological changes in the lung of OVA-induced asthma mice. (A) Representative sections of lung stained with H&E stain and M-T stain (Light microscope at 400×magnification). (B) Quantitative evaluation of the degree of lung tissue damage in the sections. Mice were induced by OVA (Control), and then treated with dexamethasone 3 mg/kg (Dexa) and GGX (100, 200, 400 mg/kg). Data are presented as mean±SE (n=8). ^†: Significant difference compared with normal group (^†††p<0.001), * : Significant difference compared with control group (*** p<0.001).
그림 Fig. 11. Immunofluorescence staining for STAT3 and GATA3 expression in the lungs of OVA-induced asthma mice model. STAT3 (A) and GATA3 (B) expressions were potently inhibited by treatment with all GGX extracts and dexamethasone. Mice were induced by OVA (Control), and then treated with dexamethasone 3 mg/kg (Dexa) and GGX (100, 200, 400 mg/kg). * : Asterisks denote bronchiole.

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