[논문]플라즈마-초음파 자극에 의한 화장품의 피부 침투 증진 연구

황윤호; 조혜연; 박유진; 장휘진; 박상효; 기재홍

doi:10.9718/jber.2023.44.4.275

플라즈마-초음파 자극에 의한 화장품의 피부 침투 증진 연구
Study for Enhanced Skin Penetration of Cosmetics by Plasma-Sono Stimuli 원문보기

Journal of biomedical engineering research : the official journal of the Korean Society of Medical & Biological Engineering, v.44 no.4, 2023년, pp.275 - 283

황윤호 (연세대학교 의공학과) , 조혜연 (연세대학교 의공학과) , 박유진 (연세대학교 의공학과) , 장휘진 (연세대학교 의공학과) , 박상효 (연세대학교 의공학과) , 기재홍 (연세대학교 의공학과)

Abstract ▼ AI-Helper

The demand for skincare has increased due to the end of the COVID-19 pandemic, leading to a focus on skincare devices and technologies designed to improve the delivery of cosmetics. Among these technologies, skincare medical devices that utilize plasma therapy (Plasma) and sonophoresis (Sono) are commonly used in dermatology clinics. However, there is still a lack of quantitative analysis for transdermal absorption effects of Plasma and Sono skincare medical devices. In this study, we quantified enhanced transdermal absorption effects of Plasma and Sono devices through in-silico and ex-vivo studies. The Sono treatment demonstrated an increased transdermal absorption effect, showing a 10~13% difference in penetration compared to the control group in the in-silico experiment, and 159% and 184% increase in the ex-vivo experiment. The Plasma treatment revealed increased transdermal absorption effects, with a 1.0~2.5% penetration difference in the in-silico experiment, and a 124% increase in the ex-vivo experiment compared to the control group. We also observed a synergistic effect from the combined treatment of Plasma and Sono, as indicated by the highest increases of 197% and 242% in penetration. Furthermore, we have determined the optimal device settings and treatment conditions for Plasma-Sono skincare medical devices. Notably, higher on/off durations (Intensity levels) and longer Sono treatments resulted in greater transdermal absorption effects.

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표/그림 (7)

표 표 1. 플라즈마, 초음파 피부미용 의료기기(Plasmapro)의 출력 사양 Table 1. Output specification of Plasma-Sono skin care medical device(Plasmapro)
그림 그림 1. 3D 프린팅 피부모사팬텀을 이용한 화장품 투과율 측정 실험. (A) 실험과정 모식도, (B) 실험과정에서 촬영된 사진 Fig. 1. Experiment for measuring the penetration of cosmetics using a 3D printing skin mimicking phantom (A) Schematic diagram, (B) Real image of the experiment
그림 그림 2. 돼지 피부모델을 활용한 Rhodamine-B 투과율 측정 실험. (A) 실험과정의 모식도, (B) 실험과정에서 촬영된 사진 Fig. 2. Experiment for measuring the penetration of Rhodamine-B using a porcine skin model: (A) Schematic diagram, (B) Real image of the experiment
그림 그림 3. 화장품 흡광도 분석을 위한 파라미터. (A) 화장품 혼합 수용액과 증류수-에탄올 수용액의 흡광도 스펙트럼, (B) 화장품 농도에 따른 흡광도 추세선 Fig. 3. Parameters for cosmetics' absorbance analysis (A) Spectrum of cosmetics mixed solution and deionized water-ethanol solution, (B) Standard curve of absorbance based on cosmetics concentration
그림 그림 4. 플라즈마, 초음파 핸드피스의 지속강도에 따른 화장품의 인공막 투과율. (A) 플라즈마 핸드피스의 지속강도에 따른 화장품 투과율, (B) 초음파 핸드피스의 지속강도에 따른 화장품 투과율(5회 반복 실험, *p<0.05, **p<0.01, ***p<0.001, ****p<0.001) Fig. 4. Penetration of cosmetics through the membrane with respect to the intensity levels of Plasma and Sono handpiece (A) Penetration of cosmetics with respect to the intensity levels of Plasma handpiece, (B) Penetration of cosmetics with respect to the intensity levels of Sono handpiece(n=5, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001)
그림 그림 5. 시간에 따른 처리 조건별 화장품의 인공막 투과율(5회 반복 실험, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001) Fig. 5. Penetration of cosmetic through membrane under different treatments over time(n=5, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001)
그림 그림 6. 처리 시간 및 처리 조건별 Rhodamine-B 돼지 피부 투과율. (A) Rhodamine-B의 돼지 피부 침투 형광 이미지, (B) Rhodamine- B의 돼지 피부 투과율(I: Control, II: Plasma 5 min, III: Sono 5 min, IV: Sono 10 min, V: Plasma 5 min + Sono 5 min, VI: Plasma 5 min + Sono 10 min) (5회 반복 실험, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001) Fig. 6. Porcine Skin Penetration of Rhodamine-B according to treatment duration and conditions: (A) Fluorescent images of Rhodamine-B permeability into porcine skin, (B) Penetration of Rhodamine-B through porcine skin(I: Control, II: Plasma 5 min, III: Sono 5 min, IV: Sono 10 min, V: Plasma 5 min + Sono 5 min, VI: Plasma 5 min + Sono 10 min) (n=5, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001)

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