[논문]고지방식이 및 일반식이 백서에 대한 중완·천추·족삼리 침자의 유효성 검증

김경수; 김명훈; 설재욱; 김은주; 손홍석; 나창수

doi:10.14406/acu.2023.015

고지방식이 및 일반식이 백서에 대한 중완·천추·족삼리 침자의 유효성 검증
The Effect of CV12, ST25, ST36 Acupuncture in General Diet and High Fat Diet Rat 원문보기

Korean journal of acupuncture, v.40 no.3, 2023년, pp.109 - 127

김경수 (동신대학교 한의과대학 한의학과) , 김명훈 (동신대학교 한의과대학 한의학과) , 설재욱 (동신대학교 한의과대학 한의학과) , 김은주 (고려대학교 식품공학과) , 손홍석 (고려대학교 식품공학과) , 나창수 (동신대학교 한의과대학 한의학과)

Abstract ▼ AI-Helper

Objectives : It was conducted to experimentally analyze the effects of acupuncture treatment at CV12, ST25, and ST36 on weight, FBCS, fat metabolism, microbiome, and metabolome changes in the general diet rat and the high-fat diet rat. Methods : It was classified into four groups: general diet & non-treatment group (ND), general diet & acupuncture treatment group (ND+AT), high-fat diet & non-treatment group (HFD), and high-fat diet & acupuncture treatment group (HFD-AT). After acupuncture treatment was performed on CV12, ST25, and ST36, changes in body weight, FBCS, fat metabolism, microbiome, and metabolome were analyzed. Results : Compared to the ND group, acupuncture treatment performed on CV12, ST25, and ST36 in the ND+AT group had no significant effect. Compared to the HFD group, CV12, ST25, and ST36 acupuncture in the HFD+AT group reduced weight, fat weight, inflammatory cytokine IL-6 expression, and lipid droplet accumulation in liver tissue. Acupuncture can promote fat metabolism and relieve inflammatory conditions. Differences in diversity between ND and HFD groups were clear in changes in microbiome, fecal metabolites, and serum metabolites. As a result of some microbiome and metabolites involved in fat decomposition, intestinal lipid absorption, and blood lipid concentration control, such as Intestinimonas, Ruminococcus 1, pyroglutamic acid, tryptophan, and inositol, it was observed that the acupuncture treatment effect was evident in the disease-induced imbalance. Conclusions : Acupuncture treatment performed on CV12, ST25, ST36 clearly observed various regulatory actions on obesity induced by high-fat diet, confirming that the action of acupuncture treatment mainly plays a role in controlling an unbalanced state.

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표/그림 (25)

표 Table 1. Classification of group names, inducements, and treatment
그림 Fig. 1. Study design and time line of the experiment.
표 Table 2. Body condition score in HFD model
표 Table 3. Physical appearance score in HFD model
표 Table 4. Stool condition score in HFD model
표 Table 5. List of primer sequences
그림 Fig. 2. Effect of ST25ㆍCV12ㆍST36 acupuncture on body weight change by week with or without obesity during the entire experimental period (upper) and 12th-week (under). Values are expressed as Mean±SE. ^##p<0.01, HFD group compared to ND group. *p<0.05, HFD-AT group compared to HFD group. ND, normal diet; ND+AT, normal diet+acupuncture treatment at ST25ㆍCV12 ㆍST36 acupoint; HFD, high fat diet; HFD-AT, high fat diet+acupuncture treatment at ST25ㆍCV12ㆍST36 acupoint; NS, not significant.
그림 Fig. 3. Effect of ST25ㆍCV12ㆍST36 acupuncture on body weight gain change by week with or without obesity during the entire experimental period (upper) and 12thweek (under). Values are expressed as Mean±SE. ^##p<0.01, HFD group compared to ND group. *p<0.05, HFD-AT group compared to HFD group. ND, normal diet; ND+AT, normal diet+acupuncture treatment at ST25ㆍCV12ㆍ ST36 acupoint; HFD, high fat diet; HFD-AT, high fat diet+acupuncture treatment at ST25ㆍCV12ㆍST36 acupoint; NS, not significant.
그림 Fig. 4. Effect of ST25ㆍCV12ㆍST36 acupuncture on fat body conditioning score (FBCS) by week with or without obesity during the entire experimental period (upper) and 12th-week (under). Values are expressed as Mean±SE. ^##p<0.01, HFD group compared to ND group. **p<0.01, HFD-AT group compared to HFD group. ND, normal diet; ND+AT, normal diet+ acupuncture treatment at ST25ㆍCV12ㆍ ST36 acupoint; HFD, high fat diet; HFD-AT, high fat diet+acupuncture treatment at ST25ㆍCV12ㆍST36 acupoint; FBCS, at body conditioning score; NS, not significant.
그림 Fig. 5. Effect of ST25ㆍCV12ㆍST36 acupuncture on food intake by week with or without obesity during the entire experimental period (upper) and 12th-week (under). Values are expressed as Mean±SE. ^##p<0.01, HFD group compared to ND group.^‡p<0.05, ND-AT group compared to ND group. ND, normal diet; ND+AT, normal diet+acupuncture treatment at ST25ㆍCV12ㆍ ST36 acupoint; HFD, high fat diet; HFD-AT, high fat diet+acupuncture treatment at ST25ㆍCV12ㆍST36 acupoint; NS, not significant.
그림 Fig. 6. Effect of ST25ㆍCV12ㆍST36 acupuncture on adipose weight with or without obesity. Values are expressed as Mean±SE. ^##p<0.01, HFD group compared to ND group. *p<0.05, HFD-AT group compared to HFD group. ND, normal diet; ND+AT, normal diet+acupuncture treatment at ST25ㆍCV12ㆍST36 acupoint; HFD, high fat diet; HFD-AT, high fat diet+acupuncture treatment at ST25ㆍCV12ㆍST36 acupoint; NS, not significant.
그림 Fig. 7. Effect of ST25ㆍCV12ㆍST36 acupuncture on Cytokine IL-6 changes in brain tissue with or without obesity. Values are expressed as Mean±SE. *p<0.05, HFD-AT group compared to HFD group. ND, normal diet; ND+AT, normal diet+ acupuncture treatment at ST25ㆍCV12ㆍST36 acupoint; HFD, high fat diet; HFD-AT, high fat diet+acupuncture treatment at ST25ㆍ CV12ㆍST36 acupoint; NS, not significant.
그림 Fig. 8. Peroxisome Proliferator-Activated Receptor-α (PPAR-α) changes. Values are expressed as Mean±SE. ^##p<0.01, HFD group compared to ND group. **p<0.01, HFD-AT group compared to HFD group. ND, normal diet; ND+AT, normal diet+acupuncture treatment at ST25ㆍCV12ㆍST36 acupoint; HFD, high fat diet; HFD-AT, high fat diet+acupuncture treatment at ST25ㆍCV12ㆍST36 acupoint; NS, not significant.
그림 Fig. 10. Effect of ST25ㆍCV12ㆍST36 acupuncture on histological observation of liver with or without obesity (hematoxylin & eosin staining, magnification ×200). Arrow: lipid droplet. ND, normal diet; ND+AT, normal diet+acupuncture treatment at ST25ㆍCV12ㆍST36 acupoint; HFD, high fat diet; HFDAT, high fat diet+acupuncture treatment at ST25ㆍCV12ㆍST36 acupoint.
그림 Fig. 9. Effect of ST25ㆍCV12ㆍST36 acupuncture on peroxisome Proliferator-Activated Receptor-γ (PPAR-γ) with or without obesity. Values are expressed as Mean±SE. ND, normal diet; ND+AT, normal diet+acupuncture treatment at ST25ㆍCV12ㆍST36 acupoint; HFD, high fat diet; HFD-AT, high fat diet+acupuncture treatment at ST25ㆍCV12ㆍST36 acupoint; NS, not significant.
그림 Fig. 11. Alpha diversity indices of the fecal microbiome of each group. Values are expressed Mean±SD. ^#p< 0.05 compared with ND group.
그림 Fig. 12. Beta indices of the fecal microbiome of each group.
그림 Fig. 13. Bacterial profile of fecal microbiome. The relative abundances are shown at the phylum level.
그림 Fig. 14. Box plots comparing the relative abundance of each group. ^#p<0.05 compared with ND group. *p<0.05 compared with HFD group.
그림 Fig. 15. Bacterial profile of fecal microbiome. The relative abundances are shown at the genus level.
그림 Fig. 16. Box plots comparing the relative abundance of each group. #p<0.05 compared with ND group. *p<0.05 compared with HFD group.
그림 Fig. 17. PCA (A) and PLS-DA (B) score plot for ND, ND+AT, HFD and HFD+AT groups derived from GC/MS data of fecal samples.
그림 Fig. 18. Bar plots comparing the relative abundance of each group. ^#p<0.05 compared with ND group. *p<0.05 compared with HFD group.
그림 Fig. 19. PCA (A) and PLS-DA (B) score plot for ND, ND+AT, HFD and HFD+AT groups derived from GC/MS data of serum samples.
그림 Fig. 20. Bar plots comparing the relative abundance of each group. ^#p<0.05 compared with ND group. *p<0.05 compared with HFD group.

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표제어: PCR

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