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수생태계 건강성 조사·평가를 위한 실내분석 정도관리 방법: 부착돌말류 영구표본 분석도구 개발
The Quality Control Method in the Laboratory Analysis of Aquatic Ecosystem Health Monitoring and Assessment: Permanent Mounting Slides Tool Development Using Benthic Attached Diatoms. 원문보기

생태와 환경 = Korean journal of ecology and environment, v.56 no.3, 2023년, pp.196 - 206  

신재기 (수생태원 한강(韓江)) ,  김난영 (건국대학교 환경보건과학과) ,  박용은 (건국대학교 사회환경공학부) ,  이경락 (국립환경과학원 물환경연구부) ,  김백호 (한양대학교 생명과학과) ,  김용재 (대진대학교 생명과학과) ,  김한순 (경북대학교 생물학과) ,  이정호 (대구대학교 생물교육과) ,  이학영 (전남대학교 생물학과) ,  황순진 (건국대학교 환경보건과학과)

초록
AI-Helper 아이콘AI-Helper

하천생태계의 주요 1차 생산자인 부착돌말류는 대부분 세포 크기가 매우 작아 관찰과 피각 계수에 고배율의 현미경적 방법을 요구하며, 그로 인해 정성적, 정량적 분석에 있어 정확도와 정밀도를 확보하기가 쉽지 않다. 본 연구는 부착돌말류를 이용한 수생태계 조사·평가에 있어 정도관리를 향상시키기 위한 방법을 제안하였다. 정도관리의 목적에 부합하기 위해, 정성 및 정량분석이 동시에 가능한 영구표본 슬라이드의 분석도구를 신규로 개발하였다. 영구표본을 제작함에 있어 커버 글라스와 슬라이드 글라스에 격자 유무의 조합으로 표본을 만들어 상호 특성을 비교하였다. 그 결과, 다른 시험 조건에 비해 유색선형 격자 슬라이드를 사용하는 방법이 가장 효과적이었다. 향후, 더 개선된 방법의 개발이 기대되지만, 본 연구에서 개발된 방법은 그 효율성과 적용성에 있어 기존 부착돌말류의 실내분석 정도관리를 개선하는 데 기여할 수 있을 것으로 판단된다.

Abstract AI-Helper 아이콘AI-Helper

Benthic attached diatoms (BADs), a major primary producer in lotic stream and river ecosystems are micro-sized organisms and require a highly magnified microscopic technique in the observation work. Thus, it is often not easy to ensure accuracy and precision in both qualitative and quantitative anal...

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